Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis
Herein is described methods for a high-sensitivity means to measure the incorporation of stable isotope labeled substrates into RNA following stable isotope probing experiments (SIP). RNA is hybridized to a set of probes such as phylogenetic microarrays and isotope incorporation is quantified such as by secondary ion mass spectrometer imaging (NanoSIMS).
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗