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Ballard, Daniel

Publications and source records attributed to Ballard, Daniel.

The impact of human expert visual inspection on the discovery of strong gravitational lenses

We investigate the ability of human ‘expert’ classifiers to identify strong gravitational lens candidates in Dark Energy Survey like imaging. We recruited a total of 55 people that completed more than 25 percent of the project. During the classification task, we present to the participants 1489 images. The sample contains a variety of data including lens simulations, real lenses, non-lens examples, and unlabelled data. We find that experts are extremely good at finding bright, well-resolved Einstein rings, while arcs with g-band signal to noise less than ~25 or Einstein radii less than ~1.2 times the seeing are rarely recovered. Very few non-lenses are scored highly. There is substantial variation in the performance of individual classifiers, but they do not appear to depend on the classifier’s experience, confidence or academic position. These variations can be mitigated with a team of 6 or more independent classifiers. Our results give confidence that humans are a reliable pruning step for lens candidates, providing pure and quantifiably complete samples for follow-up studies.

79 ASTRONOMY AND ASTROPHYSICS↗

A Cristae-Like Microcompartment in Desulfobacterota

Some Alphaproteobacteria contain intracytoplasmic membranes (ICMs) and proteins homologous to those responsible for the mitochondrial cristae, an observation which has given rise to the hypothesis that the Alphaproteobacteria endosymbiont had already evolved cristae-like structures and functions. However, our knowledge of microbial fine structure is still limited, leaving open the possibility of structurally homologous ICMs outside the Alphaproteobacteria. Here, we report on the detailed characterization of lamellar cristae-like ICMs in environmental sulfate-reducing Desulfobacterota that form syntrophic partnerships with anaerobic methane-oxidizing (ANME) archaea. These structures are junction-bound to the cytoplasmic membrane and resemble the form seen in the lamellar cristae of opisthokont mitochondria. Extending these observations, we also characterized similar structures in Desulfovibrio carbinolicus, a close relative of the magnetotactic D. magneticus, which does not contain magnetosomes. Despite a remarkable structural similarity, the key proteins involved in cristae formation have not yet been identified in Desulfobacterota, suggesting that an analogous, but not a homologous, protein organization system developed during the evolution of some members of Desulfobacterota.

electron microscope tomography↗