Search NASA⌕ Search

Engineering topics

Beller, Harry

Publications and source records attributed to Beller, Harry.

Soil Nitrogen, Water Content, Microbial Biomass, and Archaeal, Bacterial and Fungal Communities from the East River Watershed, Colorado collected in 2016-2017

This data set contains soil measurements made at the Pumphouse Hillslope to Floodplain transect at the locations of the early snowmelt-timing manipulation experiments in the East River Watershed in Colorado, USA. The data were collected in 2016 and 2017 to determine soil microbial responses to snow accumulation, snowmelt, and transition from winter to spring. Some additional data from the Washington Gulch snowmelt manipulation experiment lcoations are also included here.Data were collected on soil cores collected in the field at three soil depths (0 to 5 cm, 5 to 15 cm, 15 cm +) below the soil surface and analyzed in the on homogenized samples with roots and gravel removed. Data include gravimetric soil water content (48 hr oven-dried) and soil pH measurements (1:1 w/vol in ddH2O), dissolved nitrogen pools measured in soil extracts (2M KCl extractant), microbial biomass carbon and nitrogen (0.5M K2SO4 extractant), and archaeal, bacterial, and fungal species determined by Illumina 300 bp paired-read marker gene sequencing (16S rDNA for archaea and bacteria and ITS for fungi).

54 ENVIRONMENTAL SCIENCES↗

Sample Collection Metadata for Soil Cores from the East River Watershed, Colorado collected in 2017

This data package contains sample collection metadata for soil cores from the East River Watershed in Colorado used in biogeochemical analyses by the Watershed Function SFA. Soil cores were collected seasonally during autumn, winter, snowmelt, and spring at a predominately montane meadow, high altitude field site. Information provided for each soil core sample assigned an International Geo Sample Number (IGSN) includes: sample name, IGSN, material, description, collection method, core depth, location, field program, collectors, and date. The full list of 53 IGSN's is listed in the Methods section. There is also a complete core sample list and metadata for the set of baseline data.

54 ENVIRONMENTAL SCIENCES↗

The East River, Colorado, Watershed: A Mountainous Community Testbed for Improving Predictive Understanding of Multiscale Hydrological–Biogeochemical Dynamics

Core Ideas Development of a 300‐km 2 mountainous headwater testbed began in 2016 in the East River. The testbed can be used to explore how watershed changes impact downgradient water availability and quality. System‐of‐system, scale‐adaptive approaches can potentially improve watershed dynamics simulation. We have new approaches to monitor and simulate water partitioning and system responses. The East River watershed has been developed as a “community” testbed. Extreme weather, fires, and land use and climate change are significantly reshaping interactions within watersheds throughout the world. Although hydrological–biogeochemical interactions within watersheds can impact many services valued by society, uncertainty associated with predicting hydrology‐driven biogeochemical watershed dynamics remains high. With an aim to reduce this uncertainty, an approximately 300‐km 2 mountainous headwater observatory has been developed at the East River, CO, watershed of the Upper Colorado River Basin. The site is being used as a testbed for the Department of Energy supported Watershed Function Project and collaborative efforts. Building on insights gained from research at the “sister” Rifle, CO, site, coordinated studies are underway at the East River site to gain a predictive understanding of how the mountainous watershed retains and releases water, nutrients, carbon, and metals. In particular, the project is exploring how early snowmelt, drought, and other disturbances influence hydrological–biogeochemical watershed dynamics at seasonal to decadal timescales. A system‐of‐systems perspective and a scale‐adaptive simulation approach, involving the combined use of archetypal watershed subsystem “intensive sites” are being tested at the site to inform aggregated watershed predictions of downgradient exports. Complementing intensive site hydrological, geochemical, geophysical, microbiological, geological, and vegetation datasets are long‐term, distributed measurement stations and specialized experimental and observational campaigns. Several recent research advances provide insights about the intensive sites as well as aggregated watershed behavior. The East River “community testbed” is currently hosting scientists from more than 30 institutions to advance mountainous watershed methods and understanding.

54 ENVIRONMENTAL SCIENCES↗

Final Report: Molecular mechanisms and kinetics of microbial anaerobic nitrate-dependent U(IV) and Fe(II) oxidation

In this project, we combined molecular genetic, spectroscopic, and microscopic techniques with kinetic and reactive transport studies to describe and quantify biotic and abiotic mechanisms underlying anaerobic, nitrate-dependent U(IV) and Fe(II) oxidation, which influences the long-term efficacy of in situ reductive immobilization of uranium at DOE sites. In these studies, Thiobacillus denitrificans, an autotrophic bacterium that catalyzes anaerobic U(IV) and Fe(II) oxidation, was used to examine coupled oxidation-reduction processes under either biotic (enzymatic) or abiotic conditions in batch and column experiments with biogenically produced UIVO2(s). Synthesis and quantitative analysis of coupled chemical and transport processes were done with the reactive transport modeling code Crunchflow. Research focused on identifying the primary redox proteins that catalyze metal oxidation, environmental factors that influence protein expression, and molecular-scale geochemical factors that control the rates of biotic and abiotic oxidation.

54 ENVIRONMENTAL SCIENCES↗

SFA 2.0- Metabolic Potential

Berkeley Lab Earth Scientists Jill Banfield and Harry Beller explain the Sustainable Systems SFA 2.0 project's research on metabolic potential—or how metabolic lifestyles of microbial communities modulate in response to as well as influence environmental change.

54 ENVIRONMENTAL SCIENCES↗