Engineering topics

Burden, Hubert W.

Publications and source records attributed to Burden, Hubert W..

4 records

NASA NTRS2003

Effects of hypergravity on ovarian-hypophyseal function in antepartum and postpartum rats

BACKGROUND: Rats exposed to microgravity during the post-implantation phase of pregnancy had minimal alterations in ovarian and hypophyseal parameters during the antepartum and postpartum periods. In the current study, a similar parallel experimental design was employed to ascertain the effects of hypergravity on ovarian and hypophyseal function. HYPOTHESIS: We hypothesized that hypergravity exposure during the post-implantation stage of pregnancy would not alter antepartum and postpartum ovarian and hypophyseal function. METHODS: Pregnant rats were assigned to hypergravity (1.5 G, 1.75 G, or 2.0 G), rotational control, or stationary control groups (n = 10 each group) beginning on gestation day 11 and ending on day 20. Hypophyseal and ovarian analyses were conducted on 5 of the animals from each group at day 20. The remaining animals in each group were allowed to go to term and the same analyses were conducted 3 h postpartum. RESULTS: Hypergravity at all levels decreased the percent body mass gain from gestation day 11 to 20 (p < 0.05); however, the wet weight of the pituitaries and ovaries was not changed. There was no effect of hypergravity on the number of healthy or atretic antral follicles of any size at gestation day 20 or postpartum. The number of corpora lutea of pregnancy was decreased in all hypergravity groups, but the number of live fetuses at gestation day 20 or pups at term was not altered. Plasma concentrations of luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin, and progesterone were not changed at gestation day 20 or postpartum. Pituitary content of LH, FSH, and prolactin was not altered by hypergravity at gestation day 20, but LH content was significantly increased (p < 0.05) at 1.5 and 1.75 G postpartum. CONCLUSIONS: We conclude that hypergravity, up to and including 2.0 G, is compatible with maintenance of pregnancy and has minimal effects on hypophyseal parameters. Ovarian follicles are not altered by hypergravity, but corpora lutea may regress at a more rapid rate.

NASA Discipline Developmental Biology↗

NASA NTRS1997

Physiological Anatomical Rodent Experiment (PARE): 04 Flight Support

Rats were shipped to Kennedy Space Center (KSC), Florida, on day 2 of gestation [(G2) (day 1 = morning on which spermatozoa are present in the vagina)] and laparotomized on G7 to determine the number of implantation sites in each uterine horn. On G8, ten pregnant rats meeting flight criteria (at least five implantation sites each horn) were placed into NASA flight cages (animal enclosure modules, AEMS, five rats per cage), loaded onto the mid-deck of the space shuttle Atlantis, and on G9 (November 3, 1994) they were launched into orbit. On gestation day 20, (November 14, 1994) the shuttle returned to Edwards Air Force Base, California and the flight animals were recovered from the shuttle and subjected to unilateral hysterectomy to provide fetal material from one horn to investigators identified by NASA to study selected fetal parameters. After unilateral hysterectomy, the animals were allowed to recover and deliver vaginally. There were three groups (n = 10 each group) of control animals housed at KSC in the study. A synchronous control group, delayed 24 hours with reference to the flight group, received the same surgeries and was housed five rats per AEM and exposed to all flight conditions (identical temperatures, lighting and humidity) except microgravity. Vivarium control group I did not receive any surgery and was housed in the vivarium in clear polycarbonate cages. Vivarium control group 2 received only a unilateral hysterectomy on day 20 of gestation but otherwise was housed like the vivarium control group 1. Control groups were allowed to complete their pregnancy and deliver fetuses from the remaining horn (Synchronous control and vivarium control group 2) or horns (Vivarium control group 1). After surgery, the dams were euthanized and tissues recovered, and the neonates were assigned to foster dams.

Burden, Hubert W.↗

NASA NTRS1994

Physiological Anatomical Rodent Experiment (PARE) .04 feasibility test 2

The objective of this feasibility study was to subject pregnant rats of the same age, strain, and size that will be utilized in a shuttle flight experiment to all flight conditions except the unique microgravity of space flight and determine the feasibility of the proposed experimental design to meet the experimental objectives. The study utilized facilities at NASA, Ames Research Center, Moffett Field, CA to subject the rats to the gravitational stresses of a simulated shuttle launch and simulated shuttle landing. One hundred pregnant rats were received on gestation day (G) 2 (day 1 = day of vaginal sperm) and on G7, eighty rats were laparotomized to determine the condition of pregnancy and allow assignment to test groups. The five test groups (N=10 each group) were as follows: Group 1, Nominal Flight; Group 2, Laparotomy Control; Group 3, Hysterectomy Control; Group 4, Vivarium Control; Group 5, Delayed Recovery. On G9, animals in groups 1,2,3, and 5 were subjected to a shuttle launch simulation. On G18, groups 1,2, and 3 were subjected to a shuttle landing simulation and on this same day groups 1 and 2 were subjected to unilateral hysterectomy to obtain fetuses and placentas for evaluation. Fetal crown-rump length and fetal weight of the Nominal Flight group was significantly less than the Laparotomy Control group, but placentas were similar. On G20, group 5 was subjected to a shuttle landing simulation and on this day this group received a unilateral hysterectomy and fetuses and placentas were weighed. Animals in all groups were allowed to go to term and all animals delivered between 06:00 hours G22 and 18:00 hours G23. After delivery, a blood sample was taken from each experimental dam, and they were euthanized and the thymus and adrenal glands weighed. The thymus weight from all experimental group dams was decreased relative to the Vivarium Control group but adrenal glands and hormone values in dam plasma was similar in all groups. Pups from experimental groups were tattooed for identification, the anogenital distance of male pups was measured, and all pups placed with foster dams and litter sizes were standardized to 10. There was no difference in anogenital distances between male pups from different test groups. Pups delivered from Delayed Recovery animals were smaller than pups delivered from Nominal Flight animals. On neonatal day 7, all pups were euthanized and pup adrenal glands and thymus weighed. There was no difference in weights of thymus and adrenal glands in pups euthanized at neonatal day 7. Collectively, these data confirm the feasibility of the experimental design to meet objectives of the studies proposed for shuttle flight.

Burden, Hubert W.↗

NASA NTRS1993

Physiological Anatomical Rodent Experiment (PARE) .04 Feasibility Test 1

The objective of this feasibility study was to investigate the environmental/treatment stresses in the proposed PARE.04 experiments in a ground based study to determine if these stresses were of sufficient magnitude to compromise the planned shuttle experiments. Eighty pregnant Sprague-Dawley rats were received on day 2 (day l equals day of vaginal plug) of gestation (G2) and on G7 60 were laparotomized to determine the condition of pregnancy and allow assignment to test groups. The five test groups (N equals 10 each group) were as follows: Group 1, nominal flight; Group 2, laparotomy control; Group 3, hysterectomy control; Group 4, vivarium control; Group 5, caesarean delivery. On G17, groups 1, 2, and 5 were subjected to unilateral hysterectomy to obtain fetuses for evaluation. There was no difference in fetal crown-rump length, fetal weight, or placental weight in any of the test groups subjected to unilateral hysterectomy at G17. Animals were allowed to go to term and animals in each group delivered between the morning of G22 and the afternoon of G23. Rats assigned to Group 5 began delivering vaginally prior to the designated time for caesarean section, thus only 2 animals in this group were delivered by caesarean section. After delivery, a blood sample was taken from the dam, and they were euthanized and the thymus and adrenal glands weighed. Pups from experimental dams were tattooed for identification, the anogenital distance of male pups was photographed for later measurement, and all pups placed with foster dams and litter sizes were standardized to 10. On day 7, all pups were euthanized, and pup adrenal glands and thymus weighed. Laparotomy at G7 with or without unilateral hysterectomy at G17, had no effect on pregnancy maintenance or vaginal delivery. There was no difference in maternal adrenal or thymus weights or plasma levels of catecholamines, estradiol, progesterone, or corticosterone. Likewise, there was no difference in the anogenital distance (index of fetal stress) of neonatal male pups, between any of the experimental groups. From days 0-7, weight gain from dams in all experimental groups was similar. Lastly, there was no difference in weights of thymus and adrenal glands in pups euthanized at day 7. Collectively, these data indicate that treatment stresses inherent in the proposed PARE.04 experimental design should not compromise the planned shuttle experiments.

Burden, Hubert W.↗