Engineering topics
Chen, Zhong
Publications and source records attributed to Chen, Zhong.
Oncogenic Ras and ΔNp63α cooperate to recruit immunosuppressive polymorphonuclear myeloid-derived suppressor cells in a mouse model of squamous cancer pathogenesis
Introduction Amplification of human chromosome 3q26-29, which encodes oncoprotein ΔNp63 among other isoforms of the p63 family, is a feature common to squamous cell carcinomas (SCCs) of multiple tissue origins. Along with overexpression of ΔNp63, activation of the protooncogene, RAS , whether by overexpression or oncogenic mutation, is frequently observed in many cancers. In this study, analysis of transcriptome data from The Cancer Genome Atlas (TCGA) demonstrated that expression of TP63 mRNA , particularly ΔNp63 isoforms, and HRAS are significantly elevated in advanced squamous cell carcinomas of the head and neck (HNSCCs), suggesting pathological significance. However, how co-overexpressed ΔNp63 and HRAS affect the immunosuppressive tumor microenvironment (TME) is incompletely understood. Methods Here, we established and characterized an immune competent mouse model using primary keratinocytes with retroviral-mediated overexpression of ΔNp63α and constitutively activated HRAS (v-ras Ha G12R) to evaluate the role of these oncogenes in the immune TME. Results In this model, orthotopic grafting of wildtype syngeneic keratinocytes expressing both v-ras Ha and elevated levels of ΔNp63α consistently yield carcinomas in syngeneic hosts, while cells expressing v-ras Ha alone yield predominantly papillomas. We found that polymorphonuclear (PMN) myeloid cells, experimentally validated to be immunosuppressive and thus representing myeloid-derived suppressor cells (PMN-MDSCs), were significantly recruited into the TME of carcinomas arising early following orthotopic grafting of ΔNp63α/v-ras Ha -expressing keratinocytes. ΔNp63α/v-ras Ha -driven carcinomas expressed higher levels of chemokines implicated in recruitment of MDSCs compared to v-ras Ha -initiated tumors, providing a heretofore undescribed link between ΔNp63α/HRAS-driven carcinomas and the development of an immunosuppressive TME. Conclusion These results support the utilization of a genetic carcinogenesis model harboring specific genomic drivers of malignancy to study mechanisms underlying the development of local immunosuppression.
Structural variant analysis of a cancer reference cell line sample using multiple sequencing technologies
The cancer genome is commonly altered with thousands of structural rearrangements including insertions, deletions, translocation, inversions, duplications, and copy number variations. Thus, structural variant (SV) characterization plays a paramount role in cancer target identification, oncology diagnostics, and personalized medicine. As part of the SEQC2 Consortium effort, the present study established and evaluated a consensus SV call set using a breast cancer reference cell line and matched normal control derived from the same donor, which were used in our companion benchmarking studies as reference samples. We systematically investigated somatic SVs in the reference cancer cell line by comparing to a matched normal cell line using multiple NGS platforms including Illumina short-read, 10X Genomics linked reads, PacBio long reads, Oxford Nanopore long reads, and high-throughput chromosome conformation capture (Hi-C). We established a consensus SV call set of a total of 1788 SVs including 717 deletions, 230 duplications, 551 insertions, 133 inversions, 146 translocations, and 11 breakends for the reference cancer cell line. To independently evaluate and cross-validate the accuracy of our consensus SV call set, we used orthogonal methods including PCR-based validation, Affymetrix arrays, Bionano optical mapping, and identification of fusion genes detected from RNA-seq. We evaluated the strengths and weaknesses of each NGS technology for SV determination, and our findings provide an actionable guide to improve cancer genome SV detection sensitivity and accuracy. A high-confidence consensus SV call set was established for the reference cancer cell line. A large subset of the variants identified was validated by multiple orthogonal methods.
High Temperature Optocoupler for 3D High Density Power Modules
The goal of this proposed research is to develop a reliable high-temperature optocouplers, which can operate at 250°C with at least ten-year lifetime, and replace isolation transforms as the galvanic isolation solution for the 3D integration of high density power modules. The electrification of future transportations (i.e., electric vehicles) will continuously drive the demand for high density power modules. Optocouplers (i.e., packaged light emitter and detector) as a promising candidate to replace bulky isolation transformers are highly desirable to facilitate the continuous scale-down of gate driver circuitry that will lead to 3D high density power modules and achieve disruptive performance in terms of thermal management, power density, power efficiency, reliability and operating environments. However, regular semiconductor optoelectronic materials and devices have significant difficulty functioning in the harsh environments designated for high density power module usage (such as operation at high temperatures). Ultimately, it is not the intrinsic properties of power devices that prevent their use at higher temperatures, but rather the low voltage electronics needed to drive them and the packaging that surrounds them. The typical operating temperature for optocouplers is only up to 100°C, due to the limitations of light emitting diode (LED) devices inside and packaging materials. A systematic characterization methodology will be developed to analyze the performance, lifetime and reliability of LED devices and distinguish multiple failure mechanisms at high temperatures. An original methodology of “design for reliability” will be developed to design the optoelectronic devices with high reliability and long lifetime at high temperatures. A new architecture of high temperature high reliable optocouplers will be developed, fabricated and demonstrated with continuous operating at 250°C. The development of efficient, reliable high density 3D power modules is the foundation for energy efficiency and energy reliability. Enabled with advanced 3D integration and packaging technologies, high density power module solutions can achieve much more superior performance over the conventional discrete solutions in terms of efficiency, thermal management and power density. The proposed concept of high temperature optocouplers as the galvanic isolation solution for high density power modules will bring together interdisciplinary research involving the wide bandgap materials, optoelectronics, high reliable device design, electronics packaging and power modules. A streamline of skilled personnel would be trained including graduate and undergraduate students, local engineers and scientists which are in great demand to both academia and optoelectronics industry. The proposed research topics, such as, solid state lighting and high temperature device reliability, are currently of major interest at the Department of Energy, in particular, Sandia National Laboratories. This project can enhance collaborations between the University of Arkansas (UA) and Sandia National Laboratories. The findings of the proposed research are expected to be integrated into high density 3-D power modules at the Engineering Research Center for Power Optimization for Electro-Thermal Systems (POETS).
High temperature optoelectronic devices for power electronics
A high temperature optoelectronic isolator for power electronics operating above 250 degrees Celcius.
High-temperature analysis of optical coupling using AlGaAs/GaAs LEDs for high-density integrated power modules
A low-temperature co-fired ceramic (LTCC)-based optocoupler design is demonstrated as a possible solution for optical isolation in high-density integrated power modules. The design and fabrication of LTCC based package are discussed. Commercially available aluminum gallium arsenide/gallium arsenide (AlGaAs/GaAs) double heterostructure is used both as emitter and photodetector in the proposed optocoupler. A detailed study on the electroluminescence and spectral response of the AlGaAs/GaAs structure is conducted at elevated temperatures. The material figure of merit parameter, D*, is calculated in the temperature range 77–800 K. The fabricated optocoupler is tested at elevated temperatures, and the results are presented.
Design and optimization of high temperature optocouplers as galvanic isolation
The commercial InGaN-based (blue and green) and AlGaInP-based (red) multiple quantum well (MQW) lighting emitting diodes (LEDs) were studied in a wide range of temperatures up to 800 K for their light emission and detection (i.e., LEDs operated under reverse bias as photodiodes (PDs)) characteristics. The results indicate the feasibility of integrating a pair of selected LEDs to fabricate high temperature (HT) optocouplers, which can be utilized as galvanic isolation to replace the bulky isolation transforms in the high-density power modules. A detailed study on LEDs and PDs were performed. The external quantum efficiency (EQE) of the LED and PDs were calculated. Higher relative external quantum efficiency (EQE) and lower efficiency droops with temperatures are obtained from the blue and green LEDs for display compared with the blue one for lighting and red LED for display. The blue for lighting and red for display devices show superior responsivity, specific detectivity (D*), and EQE compared with blue and green for display when operated as PDs. The results suggest that red LED devices for display can be used to optimize HT optocouplers due to the highest wavelength overlapping compared with others.
VLBI Data Processing on Coronal Radio-sounding Experiments of Mars Express
The ESA’s Mars Express solar corona experiments were performed at two solar conjunctions in the years 2015 and 2017 by a number of radio telescopes in the European VLBI Network. This paper presents the methods to measure the frequency and phase fluctuations of the spacecraft radio signal, and the applications to study the characteristics of the plasma turbulence effects on the signal at a single station and at multiple stations via cross correlation. The power spectra of the frequency fluctuations observed between 4.9 and 76.3 R {sub s} have a power-law shape close to a Kolmogorov spectrum over the frequency interval ν {sub lo} < ν < ν {sub up}, where the nominal value of ν {sub lo} is set to 3 mHz and ν {sub up} is in the range of 0.03–0.15 Hz. The rms of the frequency fluctuations is presented as a function of the heliocentric distance. Furthermore, we analyze the variations of the electron column density fluctuations at solar offsets 4.9 R {sub s} and 9.9 R {sub s} and the cross-correlation products between the VLBI stations. The power density of the differential fluctuations between different stations decreases at ν < 0.01 Hz. Finally, the fast flow speeds of solar wind >700 km s{sup -1} are derived from the cross correlation of frequency fluctuations at ν < 0.01 Hz. The fast flow speeds of solar wind correspond to the high heliolatitude of the coronal region that the radio rays passed. The VLBI observations and analysis methods can be used to study the electron column density fluctuations and the turbulence at multiple spatial points in the inner solar wind by providing multiple lines of sight between the Earth and the spacecraft.
High-Temperature Optical Characterization of GaN-Based LEDs for Future Power Electronic Modules.
Abstract not provided.
High Temperature and Power Dependent Photoluminescence Analysis on Commercial Lighting and Display LED Materials for Future Power Electronic Modules
Commercial light emitting diode (LED) materials - blue (i.e., InGaN/GaN multiple quantum wells (MQWs) for display and lighting), green (i.e., InGaN/GaN MQWs for display), and red (i.e., Al 0.05 Ga 0.45 In 0.5 P/Al 0.4 Ga 0.1 In 0.5 P for display) are evaluated in range of temperature (77–800) K for future applications in high density power electronic modules. The spontaneous emission quantum efficiency (QE) of blue, green, and red LED materials with different wavelengths was calculated using photoluminescence (PL) spectroscopy. The spontaneous emission QE was obtained based on a known model so-called the ABC model. This model has been recently used extensively to calculate the internal quantum efficiency and its droop in the III-nitride LED. At 800 K, the spontaneous emission quantum efficiencies are around 40% for blue for lighting and blue for display LED materials, and it is about 44.5% for green for display LED materials. The spontaneous emission QE is approximately 30% for red for display LED material at 800 K. The advance reported in this paper evidences the possibility of improving high temperature optocouplers with an operating temperature of 500 K and above.
Design and Fabrication of High Temperature Optocoupler for High Density Power Module.
Abstract not provided.
High-Temperature Optical Characterization of GaN-Based Blue LEDs for Future Power Electronic Modules.
Abstract not provided.
Improvement of Stratospheric Aerosol Extinction Retrieval from OMPS/LP Using a New Aerosol Model
The Ozone Mapping and Profiler Suite Limb Profiler (OMPS/LP) has been flying on the Suomi National Polar-orbiting Partnership (S-NPP) satellite since October 2011. It is designed to produce ozone and aerosol vertical profiles at 2-kilometer vertical resolution over the entire sunlit globe. Aerosol extinction profiles are computed with Mie theory using radiances measured at 675 nanometers. The operational Version 1.0 (V1.0) aerosol extinction retrieval algorithm assumes a bimodal lognormal aerosol size distribution (ASD) whose parameters were derived by combining an in situ measurement of aerosol microphysics with the Stratospheric Aerosol and Gas Experiment (SAGE II) aerosol extinction climatology. Internal analysis indicates that this bimodal lognormal ASD does not sufficiently explain the spectral dependence of LP-measured radiances. In this paper we describe the derivation of an improved aerosol size distribution, designated Version 1.5 (V1.5), for the LP retrieval algorithm. The new ASD uses a gamma function distribution that is derived from Community Aerosol and Radiation Model for Atmospheres (CARMA)-calculated results. A cumulative distribution fit derived from the gamma function ASD gives better agreement with CARMA results at small particle radii than bimodal or unimodal functions. The new ASD also explains the spectral dependence of LP-measured radiances better than the V1.0 ASD. We find that the impact of our choice of ASD on the retrieved extinctions varies strongly with the underlying reflectivity of the scene. Initial comparisons with collocated extinction profiles retrieved at 676 nanometers from the SAGE III instrument on the International Space Station (ISS) show a significant improvement in agreement for the LP V1.5 retrievals. Zonal mean extinction profiles agree to within 10 percent between 19 and 29 kilometers, and regression fits of collocated samples show improved correlation and reduced scatter compared to the V1.0 product. This improved agreement will motivate development of more sophisticated ASDs from CARMA results that incorporate latitude, altitude and seasonal variations in aerosol properties.
Improving the Performance of the OMPS Limb Profiling Instrument in Monitoring Aerosols in the Post-Pinatubo Stratosphere
The OMPS Limb Profiling (LP) instrument was launched on NOAA's Suomi NPP (SNPP) weather satellite in October 2011. The instrument measures limb-scattered radiation in the ultraviolet, visible and NIR wavelengths with high vertical resolution (~2 km) and relatively dense spatial and temporal sampling (several thousand measurements/day covering the entire sunlit globe). These measurements have very high sensitivity to stratospheric aerosols due to long path of the incoming solar photons through the aerosol layer. However, unlike the recently launched ISS/SAGE III that directly measures extinction of solar/lunar radiation by gases and particles along the line of sight (LOS) of the instrument, information content of OMPS LP measurements is complex. It consists of solar radiation singly scattered and attenuated by aerosols, air, and trace gases along the LOS of the instrument, as well as diffuse upwelling radiation from the lower atmosphere that also gets scattered and attenuated into the LOS. To unscramble this complex signal one needs to have good knowledge of aerosol size distribution (ASD) and their complex refractive index. We will describe our recent efforts in constraining the ASD parameters by analyzing in-situ balloon data from Laramie, Wyoming; aerosol microphysical model data from CARMA; and spectral information provided by OMPS LP. We will also discuss our proposed methodology to reï¬ne this information by adjusting the microphysical properties of the aerosols to make data from various occultation and scattering instruments internally consistent. This is a generalized version of the technique employed by AERONET in which solar extinction data are combined with scattered radiation measured by the almucantar technique to determine aerosol particle shape, complex refractive index and size distribution. We suggest that such closure studies are essential to develop confidence in space-based data to validate aerosol microphysical models, such as CARMA, and aerosol chemistry- transport models, such as GOCART, and for estimating radiative forcing due to stratospheric aerosols in both volcanic and quiescent time periods.
Validation of Ozone Profile Retrievals Derived from the OMPS LP Version 2.5 Algorithm Against Correlative Satellite Measurements
The Limb Profiler (LP) is a part of the Ozone Mapping and Profiler Suite launched on board of the Suomi NPP satellite in October 2011. The LP measures solar radiation scattered from the atmospheric limb in ultraviolet and visible spectral ranges between the surface and 80 km. These measurements of scattered solar radiances allow for the retrieval of ozone profiles from cloud tops up to 55 km. The LP started operational observations in April 2012. In this study we evaluate more than 5.5 years of ozone profile measurements from the OMPS LP processed with the new NASA GSFC version 2.5 retrieval algorithm. We provide a brief description of the key changes that had been implemented in this new algorithm, including a pointing correction, new cloud height detection, explicit aerosol correction and a reduction of the number of wavelengths used in the retrievals. The OMPS LP ozone retrievals have been compared with independent satellite profile measurements obtained from the Aura Microwave Limb Sounder (MLS), Atmospheric Chemistry Experiment Fourier Transform Spectrometer (ACE-FTS) and Odin Optical Spectrograph and InfraRed Imaging System (OSIRIS). We document observed biases and seasonal differences and evaluate the stability of the version 2.5 ozone record over 5.5 years. Our analysis indicates that the mean differences between LP and correlative measurements are well within required +/- 10% between 18 and 42 km. In the upper stratosphere and lower mesosphere (>43 km) LP tends to have a negative bias. We find larger biases in the lower stratosphere and upper troposphere, but LP ozone retrievals have significantly improved in version 2.5 compared to version 2 due to the implemented aerosol correction. In the northern high latitudes we observe larger biases between 20 and 32 km due to the remaining thermal sensitivity issue. Our analysis shows that LP ozone retrievals agree well with the correlative satellite observations in characterizing vertical, spatial and temporal ozone distribution associated with natural processes, like the seasonal cycle and quasi-biennial oscillations. We found a small positive drift approx. 0.5%/yr in the LP ozone record against MLS and OSIRIS that is more pronounced at altitudes above 35 km. This pattern in the relative drift is consistent with a possible 100m drift in the LP sensor pointing detected by one of our altitude-resolving methods.
The Ozone Mapping and Profiler Suite (OMPS) Limb Profiler (LP) Version 1 Aerosol Extinction Retrieval Algorithm: Theoretical Basis
The theoretical basis of the Ozone Mapping and Profiler Suite (OMPS) Limb Profiler (LP) Version 1 aerosol extinction retrieval algorithm is presented. The algorithm uses an assumed bimodal lognormal aerosol size distribution to retrieve aerosol extinction profiles at 675 nm from OMPS LP radiance measurements. A first-guess aerosol extinction profile is updated by iteration using the Chahine nonlinear relaxation method, based on comparisons between the measured radiance profile at 675 nm and the radiance profile calculated by the Gauss Seidel limb-scattering (GSLS) radiative transfer model for a spherical-shell atmosphere. This algorithm is discussed in the context of previous limb-scattering aerosol extinction retrieval algorithms, and the most significant error sources are enumerated. The retrieval algorithm is limited primarily by uncertainty about the aerosol phase function. Horizontal variations in aerosol extinction, which violate the spherical-shell atmosphere assumed in the version 1 algorithm, may also limit the quality of the retrieved aerosol extinction profiles significantly.
Genomic, Pathway Network, and Immunologic Features Distinguishing Squamous Carcinomas
Abstract not provided
25 Mg NMR and Computational Modeling Studies of the Solvation Structures and Molecular Dynamics in Magnesium Based Liquid Electrolytes
There is increasing evidence that the solvation structure of the active components in a liquid electrolyte solution strongly impacts the performance in electrochemical applications. In this work, the nanoscale solvation structures and dynamics of Mg(BH 4 ) 2 and Mg(TFSI) 2 dissolved in diglyme (DGM) at various concentrations and ratios of Mg(BH 4 ) 2 /Mg(TFSI) 2 were investigated using a combination of natural abundance 25 Mg NMR, quantum chemistry calculations of 25 Mg NMR chemical shifts, classical molecular dynamics (MD) calculations, and electrochemical performance tests. By mixing two competing Mg salts, we were able to reduce the strong covalent interactions between Mg 2+ and BH 4 – anions. A small increase is observed in the coordination number of Mg-TFSI and a significant increase in the interaction of Mg 2+ ions with glymes. Through a combination of NMR, DFT and MD simulations, various stable species around 1 nm in size were detected in the mixed salt solution, which play key roles in the enhanced electrochemical performance of the mixed electrolyte. It is established that for the neat Mg(TFSI) 2 in DGM electrolyte at dilute concentrations the TFSI - is fully dissociated from Mg 2+ . At higher concentrations, Mg 2+ and TFSI - are only partially dissociated as contact ion pairs are formed. In contrast, at 0.01 M Mg(BH 4 ) 2 (saturated concentration) in DGM, the first solvation shell of a Mg 2+ ion contains two BH 4 - anions and one DGM molecule, while the second solvation shell consists of five to six DGM molecules. An exchange mechanism between the solvation structures in the combined electrolyte containing both Mg(BH 4 ) 2 and Mg(TFSI) 2 in DGM was found to result in the observation of a single 25 Mg NMR peak. This exchange is responsible for an increase in uncoordinated anions, as well as improved stability and ionic conductivity as compared to single anion solution. Solvent molecule rearrangement and direct Mg-ion exchange between the basic solvation structures are hypothesized as likely reasons for the exchange. We elucidate that the solvent rearrangement is energetically much more favorable than direct Mg-ion hopping and is thus suggested as the dominant exchange mechanism.