Search NASA⌕ Search

Engineering topics

Chiba, A.

Publications and source records attributed to Chiba, A..

Spectroscopy of A=9 hyperlithium with the $(e,e^{\prime}K^{+})$ reaction

Missing mass spectroscopy with the $(e,e^{\prime}K^{+})$ reaction was performed at JLab Hall C for the neutron rich $\Lambda$ hypernucleus $^{9}_{\Lambda}{\rm Li}$. The ground state energy was obtained to be $B_{\Lambda}^{\rm g.s.}=8.84\pm0.17^{\rm stat.}\pm0.15^{\rm sys.}~{\rm MeV}$ by using shell model calculations of a cross section ratio and an energy separation of the spin doublet states ($3/2^{+}_1$ and $5/2^{+}_1$). In addition, peaks that are considered to be states of [$^{8}{\rm Li}(3^{+})\otimes s_{\Lambda}=3/2^{+}_{2}, 1/2^{+}$] and [$^{8}{\rm Li}(1^{+})\otimes s_{\Lambda}=5/2^{+}_{2}, 7/2^{+}$] were observed at $E_{\Lambda}(\#2)=1.74\pm0.27^{\rm stat.}\pm0.11^{\rm sys.}~{\rm MeV}$ and $E_{\Lambda}(\#3)=3.30\pm0.24^{\rm stat.}\pm0.11^{\rm sys.}~{\rm MeV}$, respectively. The $E_{\Lambda}(\#3)$ is larger than shell model predictions by a few hundred keV, and the difference would indicate that a ${\rm ^{5}He}+t$ structure is more developed for the $3^{+}$ state than those for the $2^{+}$ and $1^{+}$ states in a core nucleus $^{8}{\rm Li}$ as a cluster model calculation suggests.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Targeted gene expression without a tissue-specific promoter: creating mosaic embryos using laser-induced single-cell heat shock

We have developed a method to target gene expression in the Drosophila embryo to a specific cell without having a promoter that directs expression in that particular cell. Using a digitally enhanced imaging system to identify single cells within the living embryo, we apply a heat shock to each cell individually by using a laser microbeam. A 1- to 2-min laser treatment is sufficient to induce a heat-shock response but is not lethal to the heat-shocked cells. Induction of heat shock was measured in a variety of cell types, including neurons and somatic muscles, by the expression of beta-galactosidase from an hsp26-lacZ reporter construct or by expression of a UAS target gene after induction of hsGAL4. We discuss the applicability of this technique to ectopic gene expression studies, lineage tracing, gene inactivation studies, and studies of cells in vitro. Laser heat shock is a versatile technique that can be adapted for use in a variety of research organisms and is useful for any studies in which it is desirable to express a given gene in only a distinct cell or clone of cells, either transiently or constitutively, at a time point of choice.

Non-NASA Center↗