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Christina Johnson

Publications and source records attributed to Christina Johnson.

12 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS2022

Plasma Assisted Nutrient Recovery from Inedible Biomass via Ash Leaching

Sustaining a human presence on the Moon, Mars, or deep space will require closing loops on many life support systems. Some form of agriculture will be required because plants produce the vitamins, antioxidants, and essential oils in our diets that degrade over time in stored foods. In addition, they provide dietary fiber, restore air, and purify water. Growing plants will require recycling nutrients trapped in inedible vegetation. Researchers at KSC have investigated the use of a thermal plasma with various carrier gases to thermally degrade inedible plant biomass for nutrient recovery. Previous work demonstrated a thermally degrading environment such as a muffle furnace improved nutrient recovery from inedible biomass prior to an acid leaching process. However, a muffle furnace is an inefficient process. We have explored the use of a small scale, thermal plasma for degradation of pellets to enhance the breakdown of plant stems, leaves, and debris to further close the nutrient loop. Plasma carrier gases such as carbon dioxide (CO2), nitrogen (N2), and air were used to explore variations in recovery and potential chemical by-products. Plasma processed inedible biomass was added to varying concentrations of acid solution for leaching of nutrients (e.g. potassium (K) , magnesium (Mg), calcium (Ca), and phosphorus (P)) for reuse in the crop production cycle. We also examined total N2 recovery. Results are presented showing the impact of plasma processing prior to acid leaching on recovery of plant nutrients.

Kenneth Engeling↗
NASA NTRS2022

Microgreens Root Zone/Shoot Zone Partitioned Planting Box

To enable sustainable food production in future human exploration missions, plant growth is being studied by the Space Crop Production Team at KSC. Microgreens are good candidates for food supplements and contain specific nutrients that are lacking in the prepackaged diet, including vitamin C and vitamin K. Because they are densely sown, typical growth methods do not allow the ability to distinguish between the levels of evaporation from the rooting substrate and transpiration from the leaves. With larger plants, the root and shoot zone can be separated to distinguish these fluxes and accurate transpiration measurements of plant canopies are feasible. Furthermore, separation of the root and shoot zone may also be beneficial when harvesting microgreens in microgravity as it may reduce microbial contamination of the edible biomass by the roots, which have high microbial loads. Using a root and shoot separator box when harvesting may help with microbial contamination, but these tiny plants are challenging to handle in microgravity, so harvest management remains an open question. The innovation proposed here is a microgreen root/shoot partitioned planting box, which offers a solution to these challenges with accurate gas exchange measurements and a safe microgreen harvest in low gravity environments. Being able to measure transpiration of a microgreen canopy will be important for modeling plant growth in reduced gravity environments, so the first objective was to develop a planting unit with a seal between the root zone and the shoot zone. With an unsuitable harvesting technique, freshly harvested microgreens may add debris to the cabin, so the second objective was to test different harvesting techniques and management approaches associated with this innovation. These two objectives were pursued in parallel since many goals were the same: develop a planting unit that 1) separates the shoots from the roots, 2) allows acceptable germination rate, and 3) allows for seedlings to emerge and develop. What differed was the need to have a seal, which was only applicable for our gas exchange goal, and the need to have an embedded harvesting mechanism and bagging method which only applied to our harvesting goal. Testing of the various harvesting mechanisms and bagging methods was performed during a series of parabolic flights. All parabolic flight procedures took place inside a rented secondary containment chamber (e.g., glovebox) that was developed by the University of Louisville specifically for experiments involving fluids and other materials that may become airborne during reduced gravity flight. Three different harvesting methods and two different bagging collection methods were tested for microgreens. A third bagging method was initially tested but found to be unsuitable. Human factors were also taken into consideration, to identify which harvest and bagging collection methods would be easiest to use with favorable results in microgravity. Three parabolic flight tests were performed in total, one in November 2021 and two in December 2021.

Gioia Massa↗
NASA NTRS2021

Customized Science Carrier Modules and Accessories Developed for Microgravity Simulation Devices

Because the access to the true weightless environment of the ISS and other Low Earth Orbit (LEO) vehicles is limited and cost prohibitive, the need for ground-based microgravity simulation analogs to bridge the gravity continuum gap between 1g gravity and costly space flight studies with true weightlessness was realized. As such, the Microgravity Simulation Support Facility (MSSF) was established at NASA John F. Kennedy Space Center (KSC) to fulfill the need of the scientific community. Our facility provides the US science community with a focal point in the field of gravitational research by granting access to an array of two-dimensional(2D) and three-dimensional (3D)clinostats, and Random Positioning Machine (RPM) platforms developed by teams throughout the world. The availability of these capabilities will allow further understanding the role of altered gravity vectors from “functional weightlessness”, partial gravity conditions (1 x 10-3to 0.9g, and Moon and Mars ‘g vectors), to hypergravity (up to3g) on biological processes. We have designed and 3D printed a variety of specialized attachments(and associated “static control” hardware)to allow researchers to mount their samples onto these microgravity simulation devices to meet their unique science requirements. A summary of each attachment module will be presented below. Note: All software or code information should be requested to the author Ye Zhang, Kennedy Space Center ye.zhang-1@nasa.gov

Ye Zhang↗
NASA NTRSDate not supplied

Morphological and developmental variation of red mizuna grown under blue light in ground-based microgravity analogues through the utilization of specialized clinostats

Microgreens, such as red (purple) mizuna, have been considered as spaceflight candidate crop plants to be integrated in bioregenerative life support systems for long-duration spaceflight. This is due to their high nutrient density, relatively fast developmental cycles, low horticultural energy, water needs and minimal space requirements. It is therefore important to develop space plant life-support systems capable of mitigating any potentially detrimental effects associated with crop responses to spaceflight related stressors; such as microgravity and light variation. This study focuses on identifying phenotypic variation in the development of red (purple) mizuna grown under blue light in a 2D clinostat to simulate the lack of a gravity vector found within true microgravity.

Gilbert Cauthorn↗
NASA NTRSDate not supplied

Advantages of Microgreen Carotenoid Composition for Space Travel

Microgreens are attractive space crop candidates for their ease of maintenance and nutrient density. These tender greens are particularly rich phytochemicals like carotenoids. To determine the effect of space conditions on carotenoid synthesis, this NASA funded research used Gilroy’s Life Sciences TOAST database to compare transcriptomic data of Arabidopsis thaliana grown on previous spaceflight missions. Of the eight genes studied that involve carotenoid biosynthesis, it was determined that most were upregulated.

microgreens↗
NASA NTRSDate not supplied

Mining the Gravity Mutants of Arabidopsis

Gravity mutants are a valuable resource for understanding gravity perception, signaling, and response in plants. A review of 190 publications resulted in a list of 97 loci with mutations that caused a gravitropic phenotype. While all these mutants show some form of gravity phenotype, several are also generally defective in growth. After removing these nonspecific mutants, 76 loci were deemed to have true gravity mutants. The gene list was then used to create a sortable database containing key factors of gravity signaling as well as data on methodologies and the development of mutant lines. Indexing this database has allowed us to pull out trends that were not visible in individual publications. For example, mutants that are unable to rearrange starch statoliths make up a larger proportion of the described inflorescence stems mutants than any other organ. Comparing these statolith mutants across organs shows that root and inflorescence stems consistently display different phenotypes for the same class of defect. The more severe gravity defects are most commonly described in root tissue while other tissues are more likely to show only a delayed or reduced response to gravity. The experience of members of the GeneLab plant Analytics Working Group (AWG) in data visualization and gene mapping have sparked new ideas for utilizing this database. Knowledge of shared traits, mutant development, and growth can provide a new resource for the production of seed lines specialized for their response to gravity.

gravitropism↗
NASA NTRSDate not supplied

Plant Habitat 04 Citizen Outreach: The Space Chile Grow a Pepper Plant Challenge (2020-2021)

The Space Chile Grow a Pepper Plant Challenge (SCGAPPC) is a citizen outreach activity performed in conjunction with NASA technical demonstration, Plant Habitat (PH)-04 which grew a New Mexico chile pepper as the first fruit in the Advanced Plant Habitat (APH). Growing a related pepper variety from Chimayo, New Mexico, the activity tasked citizen “Space Farmers” to grow a pepper plant indoors, at home, or in controlled environments to document the details of growth, and search for solutions to growing consistently productive and flavorful peppers under these conditions. Launched in February of 2020, a scientist on the PH04 team shared an introduction on social media to provide information about the activity and instructions on how to participate. Each space farmer received an envelopecontaining seeds and a mission sticker. They conducted their own pepper growth trials to record environment and horticultural data and submit one pepper to the University of New Mexico for capsaicin analysis. This activity was available online at the onset of the COVID-19 shutdown. During 2020-2021, over 1,800 envelopes were mailed across the globe, tens of thousands of seed pouches were shared, the SCGAPPC was featured during a virtual Growing Beyond Earth Educator Workshop with the challenge of becoming a critical part of that program when students were working remotely. Citizen space farmers around the world contributed to space biology and crop production research while learning about CE agriculture. Members of the space biology community volunteered participation, skills, and interaction. An online community page provided a format for scientists, and the general publicto interact. A portion of data was documented in an online database. The primary outcome served to inform the publicabout NASA space crop production, while creating a channel to share experiences and compare results from a singlecrop growing under varying controlled environments.

Jacob Torres↗
NASA NTRSDate not supplied

Evaluating Microgreens Crop Readiness for Space Production.

Microgreens are small-size, nutrient-rich, and fast-grown crops, which are considered as candidates for future space exploration missions. In particular, the ISS, the Lunar Gateway, and Mars and Lunar missions could benefit from growing microgreens to supplement astronaut diets in the near future. Research at NASA’s Kennedy Space Center has focused on (1) the selection of microgreens compatible species, (2) the evaluation of microgreens food safety, (3) the use of passive wicking, on-demand watering, and hydroponics cultivation, (4) simulated microgravity growth, (5) microgreen canopy gas exchange, and (6) harvesting techniques in microgravity. This presentation summarizes this research. Microgreen species will be evaluated for their yield in relationship to the quantity of inputs – water, seeds, substrate, light intensity, photoperiod, crew time – required for their growth; for their organoleptic and sensory factors in order to down select species that are highly acceptable for humans; and for their microbial loads as detected in their growth environment and the food safety metrics of their edible tissue. Passive wicking, on-demand watering, and hydroponic systems are being studied as an efficient way to deliver essential nutrients and water to microgreens, included in a microgravity environment. Growth studies in simulated microgravity (using 3-dimensional clinostats) will assess microgreens growth relative to that in 1g. Gas exchange studies on microgreens canopies in various airflows will assess their photosynthesis and transpiration. Finally, a series of parabolic flights has enabled the evaluation of different harvesting and bagging techniques in microgravity. Indeed, traditional plant harvesting methods (scissors) in microgravity could generate significant microgreen debris in the space station cabin. Two innovative techniques, coupled to a dedicated bagging method, were designed and evaluated against the control, traditional, harvesting technique. This research was supported by grants from NASA KSC’s Independent Research and Technology Development Program, NASA’s Flight Opportunity Program, NASA Postdoctoral Program Fellowships (L.P. & C.J.) supported by NASA’s Space Biology program, and support from NASA’s Human Research Program.

crop↗
NASA NTRSDate not supplied

Plasma Activated Water: A Technology for Acid Generation and Space Crop Production

As humanity returns to the moon and onward to Mars, sustainable space travel becomes essential for cost and independence from Earth. Astronaut activities in low Earth orbit resupply consumable materials and can require alternative technologies if transportation of chemicals is deemed hazardous. Plasma technology and applications are able to generate commodities that assist in achieving Earth independence. Plasma interaction with water generates acid in-situ using on-board resources of electricity and breathable air without the need for large infrastructure. At Kennedy Space Center, researchers have shown that plasma is able to produce nitric acid, and therefore nitrates, in a plant-usable form in water. In this report, the team presents results on acid generation in g/kW-hr as well as provides results on plasma activated water for plant growth assistance. Plasma was generated utilizing DC, AC, and pulsed power supplies in order to determine optimizing parameters for generation of this useful commodity.

Plasma↗