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Collins, Robert E.

Publications and source records attributed to Collins, Robert E..

6 records
All sourcesNASA NTRSDOE OSTI
DOE OSTI2021

Structural analysis and biochemical properties of laccase enzymes from two Pediococcus species

Prokaryotic laccases are emergent biocatalysts. However, they have not been broadly found and characterized in bacterial organisms, especially in lactic acid bacteria. Recently, a prokaryotic laccase from the lactic acid bacterium Pediococcus acidilactici 5930, which can degrade biogenic amines, was discovered. Thus, our study aimed to shed light on laccases from lactic acid bacteria focusing on two Pediococcus laccases, P. acidilactici 5930 and Pediococcus pentosaceus 4816, which have provided valuable information on their biochemical activities on redox mediators and biogenic amines. Both laccases are able to oxidize canonical substrates as ABTS, ferrocyanide and 2,6-DMP, and non-conventional substrates as biogenic amines. With ABTS as a substrate, they prefer an acidic environment and show sigmoidal kinetic activity, and are rather thermostable. Moreover, this study has provided the first structural view of two lactic acid bacteria laccases, revealing new structural features not seen before in other well-studied laccases, but which seem characteristic for this group of bacteria. We believe that understanding the role of laccases in lactic acid bacteria will have an impact on their biotechnological applications and provide a framework for the development of engineered lactic acid bacteria with enhanced properties.

36 MATERIALS SCIENCE↗
NASA NTRS1990

Event driven executive

Tasks may be planned for execution on a single processor or are split up by the designer for execution among a plurality of signal processors. The tasks are modeled using a design aid called a precedence graph, from which a dependency table and a prerequisite table are established for reference within each processor. During execution, at the completion of a given task, an end of task interrupt is provided from any processor which has completed a task to any and all other processors including itself in which completion of that task is a prerequisite for commencement of any dependent tasks. The relevant updated data may be transferred by the processor either before or after signalling task completion to the processors needing the updated data prior to commencing execution of the dependent tasks. Coherency may be ensured, however, by sending the data before the interrupt. When the end of task interrupt is received in a processor, its dependency table is consulted to determine those tasks dependent upon completion of the task which has just been signalled as completed, and task dependency signals indicative thereof are provided and stored in a current status list of a prerequisite table. The current status of all current prerequisites are compared to the complete prerequisites listed for all affected tasks and those tasks for which the comparison indicates that all prerequisites have been met are queued for execution in a selected order.

Tulpule, Bhalchandra R.↗
NASA NTRS1989

Evaluation of leading- and trailing-edge flaps on flat and cambered delta wings at supersonic speeds

An experimental investigation has been conducted to evaluate the effectiveness of leading- and trailing-edge flaps on a flat and cambered wing at superconic speeds. Results from the experimental tests showed that highly complex and three-dimensional flow can occur over the wings with leading- and/or trailing-edge flaps deflected. An analysis of the data also showed that flap effectiveness varies significantly between a cambered and flat wing of identical planform and flap geometry. Mach number effects are similar for both flat and cambered wings for all aerodynamic parameters.

Hernandez, Gloria↗
NASA NTRS1988

Symmetrization for redundant channels

A plurality of redundant channels in a system each contain a global image of all the configuration data bases in each of the channels in the system. Each global image is updated periodically from each of the other channels via cross channel data links. The global images of the local configuration data bases in each channel are separately symmetrized using a voting process to generate a system signal configuration data base which is not written into by any other routine and is available for indicating the status of the system within each channel. Equalization may be imposed on a suspect signal and a number of chances for that signal to heal itself are provided before excluding it from future votes. Reconfiguration is accomplished upon detecting a channel which is deemed invalid. A reset function is provided which permits an externally generated reset signal to permit a previously excluded channel to be reincluded within the system. The updating of global images and/or the symmetrization process may be accomplished at substantially the same time within a synchronized time frame common to all channels.

Tulplue, Bhalchandra R.↗
NASA NTRS1988

Equalization in redundant channels

A miscomparison between a channel's configuration data base and a voted system configuration data base in a redundant channel system having identically operating, frame synchronous channels triggers autoequalization of the channel's historical signal data bases in a hierarchical, chronological manner with that of a correctly operating channel. After equalization, symmetrization of the channel's configuration data base with that of the system permits upgrading of the previously degraded channel to full redundancy. An externally provided equalization command, e.g., manually actuated, can also trigger equalization.

Tulpule, Bhalchandra R.↗