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Dahlgren, Robert

Publications and source records attributed to Dahlgren, Robert.

Estimating the Relative Water Content of Leaves in a Cotton Canopy

Remotely sensing plant canopy water status remains a long-term goal of remote sensing research. Established approaches to estimating canopy water status the Crop Water Stress Index, the Water Deficit Index and the Equivalent Water Thickness involve measurements in the thermal or reflective infrared. Here we report plant water status estimates based upon analysis of polarized visible imagery of a cotton canopy measured by ground Multi-Spectral Polarization Imager (MSPI). Such estimators potentially provide access to the plant hydrological photochemistry that manifests scattering and absorption effects in the visible spectral region.Twice during one day, +- 3 hours from solar noon, we collected polarized imagery and relative water content data on a cotton test plot located at the Arid Land Agricultural Research Center, United States Department of Agriculture, Maricopa, AZ. The test plot, a small portion of a large cotton field, contained stressed plants ready for irrigation. The evening prior to data collection we irrigated several rows of plants within the test plot. Thus, ground MSPI imagery from both morning and afternoon included cotton plants with a range of water statuses. Data analysis includes classifying the polarized imagery into sunlit reflecting, sunlit transmitting, shaded foliage and bare soil. We estimate the leaf surface reflection and interior reflection based upon the per pixel polarization and sunview directions. We compare our cotton results with our prior polarization results for corn and soybean leaves measured in the lab and corn leaves measured in the field.

Cotton Canopy↗

Estimating Leaf Water Status from Vis-Nir Reflectance and Transmittance

Remotely sensing the water status of plant canopies remains a long term goal of remote sensing research. Established approaches involve measurements in the thermal infrared and the 900-2000nm reflective infrared. Less popular UV-visible-NIR techniques presumably deserve research attention, because photochemical changes linked to plant water status manifest spectral light scattering and absorption changes. Here we monitored the visible and NIR light reflected from the leaf interior as well as the leaf transmittance as the relative water content of corn (Zeamays L.) leaves decreased. Our results highlight the importance of both scattering effects and effects due to absorption by leaf pigments.

VIS-NIR Reflectance↗

Spectrometer for Sky-Scanning, Sun-Tracking Atmospheric Research (4STAR) Instrument Improvements

The Spectrometer for Sky-Scanning, Sun-Tracking Atmospheric Research (4STAR) combines airborne sun tracking and sky scanning with grating spectroscopy to improve knowledge of atmospheric constituents and their links to air-pollution and climate. Hyper-spectral measurements of direct-beam solar irradiance provide retrievals of gas constituents, aerosol optical depth, and aerosol and thin cloud optical properties. Sky radiance measurements in the principal and almucantar planes enhance retrievals of aerosol absorption, aerosol type, and size mode distribution. Zenith radiance measurements are used to retrieve cloud properties and phase, which in turn are used to quantify the radiative transfer below cloud layers. These airborne measurements tighten the closure between satellite and ground-based measurements. In contrast to the Ames Airborne Tracking Sunphotometer (AATS-14) predecessor instrument, new technologies for each subsystem have been incorporated into 4STAR. In particular, 4STAR utilizes a modular sun-trackingsky-scanning optical head with fiber optic signal transmission to rack mounted spectrometers, permitting miniaturization of the external optical head, and spectrometerdetector configurations that may be tailored for specific scientific objectives. This paper discusses technical challenges relating to compact optical collector design, radiometric dynamic range and stability, and broad spectral coverage at high resolution. Test results benchmarking the performance of the instrument against the AATS-14 standard and emerging science requirements are presented.

Atmopheric Research (4STAR)↗

Coastal California's Fog as a Unique Habitable Niche: Design for Autonomous Sampling and Preliminary Aerobiological Characterization

Just as on the land or in the ocean, atmospheric regions may be more or less hospitable to life. The aerobiosphere, or collection of living things in Earth's atmosphere, is poorly understood due to the small number and ad hoc nature of samples studied. However, we know viable airborne microbes play important roles, such as providing cloud condensation nuclei. Knowing the distribution of such microorganisms and how their activity can alter water, carbon, and other geochemical cycles is key to developing criteria for planetary habitability, particularly for potential habitats with wet atmospheres but little stable surface water. Coastal California has regular, dense fog known to play a major transport role in the local ecosystem. In addition to the significant local (1 km) geographical variation in typical fog, previous studies have found that changes in height above surface of as little as a few meters can yield significant differences in typical concentrations, populations and residence times. No single current sampling platform (ground-based impactors, towers, balloons, aircraft) is capable of accessing all of these regions of interest.A novel passive fog and cloud water sampler, consisting of a lightweight passive impactor suspended from autonomous aerial vehicles (UAVs), is being developed to allow 4D point sampling within a single fog bank, allowing closer study of small-scale (100 m) system dynamics. Fog and cloud droplet water samples from low-altitude aircraft flights in nearby coastal waters were collected and assayed to estimate the required sample volumes, flight times, and sensitivity thresholds of the system under design.125 cloud water samples were collected from 16 flights of the Center for Interdisciplinary Remotely Piloted Aircraft Studies (CIRPAS) instrumented Twin Otter, equipped with a sampling tube collector, occurring between 18 July and 12 August 2016 below 1 km altitude off the central coast. The collector was flushed first with 70 ethanol, then with sterile DI water, between sampling regions. Collected volumes ranged from 100 L to 12 mL. All samples were diluted serially and plated on two different types of agar, nutrient-dense (PCA) and sparse (R-2A). Plates were incubated at room temperature and counted when colonies first appeared and again at 2 weeks.Preliminary results from seven flights are consistent with generally reported colony-forming unit (CFU) values for terrestrial fog water (e.g., [4]). The PCA assay ranged from 400 to 125,000 CFU/mL, R-2A from 700 to 130,000 CFU/mL. PCA and R-2A counts were not significantly different from each other at I^ plus or minus +/- 0.05, although observationally, the R2A plates had more pigmented colonies. CFU counts from the majority of flights were not different from each other in mean at the same level of significance, but about half differed in median, indicating differences in underlying distribution. These results validate the presence of viable microorganisms in coastal California fog at levels that should be easily detectable by our sampling system. The indicated distribution differences underscore the need for small-scale, long-term sampling surveys. Future planned work includes ion chromatography for limiting nutrients, ATP quantification, and qPCR for several microbial classes of interest.

Coastal↗

The Don Edwards San Francisco Bay National Wildlife Refuge: A Natural Laboratory for Federal Agencies and Partners

The Don Edwards San Francisco Bay National Wildlife Refuge encompasses a rich mosaic of habitats and is a fecund and convenient field site for Earth science research, comprised of tidal salt marsh, open ponds, shallow water, mudflat habitat and encompassing a variety of tide, salinity, elevation, slope, and other conditions. Proximity to the NASA Ames Research Center and overlap with the center's UAS (unmanned aircraft systems) test area presents the opportunity to create a unique natural laboratory that can incubate and support a variety of research and benefit the Refuge in monitoring wildlife changes and habitat evolution over time.

Unmanned Airborne Vehicle↗

NH11B-1726: FrankenRaven: A New Platform for Remote Sensing

Small, modular aircraft are an emerging technology with a goal to maximize flexibility and enable multi-mission support. This reports the progress of an unmanned aerial system (UAS) project conducted at the NASA Ames Research Center (ARC) in 2016. This interdisciplinary effort builds upon the success of the 2014 FrankenEye project to apply rapid prototyping techniques to UAS, to develop a variety of platforms to host remote sensing instruments. In 2016, ARC received AeroVironment RQ-11A and RQ-11B Raven UAS from the US Department of the Interior, Office of Aviation Services. These aircraft have electric propulsion, a wingspan of roughly 1.3m, and have demonstrated reliability in challenging environments. The Raven airframe is an ideal foundation to construct more complex aircraft, and student interns using 3D printing were able to graft multiple Raven wings and fuselages into FrankenRaven aircraft. Aeronautical analysis shows that the new configuration has enhanced flight time, payload capacity, and distance compared to the original Raven. The FrankenRaven avionics architecture replaces the mil-spec avionics with COTS technology based upon the 3DR Pixhawk PX4 autopilot with a safety multiplexer for failsafe handoff to 2.4 GHz RC control and 915 MHz telemetry. This project demonstrates how design reuse, rapid prototyping, and modular subcomponents can be leveraged into flexible airborne platforms that can host a variety of remote sensing payloads and even multiple payloads. Modularity advances a new paradigm: mass-customization of aircraft around given payload(s). Multi-fuselage designs are currently under development to host a wide variety of payloads including a zenith-pointing spectrometer, a magnetometer, a multi-spectral camera, and a RGB camera. After airworthiness certification, flight readiness review, and test flights are performed at Crows Landing airfield in central California, field data will be taken at Kilauea volcano in Hawaii and other locations.

remote sensing↗

Estimating the Relative Water Content of Single Leaves from Optical Polarization Measurements

Remotely sensing the water status of plants and the water content of canopies remain long-term goals of remote sensing research. For monitoring canopy water status, existing approaches such as the Crop Water Stress Index and the Equivalent Water Thickness have limitations. The CWSI does not work well in humid regions, requires estimates of the vapor pressure deficit near the canopy during the remote sensing over-flight and, once stomata close, provides little information regarding the canopy water status. The EWI is based upon the physics of water-light interaction, not plant physiology. In this research, we applied optical polarization techniques to monitor the VISNIR light reflected from the leaf interior, R, as well as the leaf transmittance, T, as the relative water content (RWC) of corn (Zea mays) leaves decreased. Our results show that R and T both changed nonlinearly as each leaf dried, R increasing and T decreasing. Our results tie changes in the VISNIR R and T to leaf physiological changes linking the light scattered out of the drying leaf interior to its relative water content and to changes in leaf cellular structure and pigments. Our results suggest remotely sensing the physiological water status of a single leaf and perhaps of a plant canopy might be possible in the future. However, using our approach to estimate the water status of a leaf does not appear possible at present, because our results display too much variability that we do not yet understand.

Leaf Reflectance↗

Potential Sources of Polarized Light from a Plant Canopy

Field measurements have demonstrated that sunlight polarized during a first surface reflection by shiny leaves dominates the optical polarization of the light reflected by shiny-leafed plant canopies having approximately spherical leaf angle probability density functions ("Leaf Angle Distributions" - LAD). Yet for other canopies - specifically those without shiny leaves and/or spherical LADs - potential sources of optically polarized light may not always be obvious. Here we identify possible sources of polarized light within those other canopies and speculate on the ecologically important information polarization measurements of those sources might contain.

Polarized Light↗

Leaf Relative Water Content Estimated from Leaf Reflectance and Transmittance

Remotely sensing the water status of plants and the water content of canopies remain long term goals of remote sensing research. In the research we report here, we used optical polarization techniques to monitor the light reflected from the leaf interior, R, as well as the leaf transmittance, T, as the relative water content (RWC) of corn (Zea mays) leaves decreased. Our results show that R and T both change nonlinearly. The result show that the nonlinearities cancel in the ratio R/T, which appears linearly related to RWC for RWC less than 90%. The results suggest that potentially leaf water status and perhaps even canopy water status could be monitored starting from leaf and canopy optical measurements.

Corn↗

Relative Water Content, Bidirectional Reflectance and Bidirectional Transmittance of the Interior of Detached Leaves During Dry Down.

Remotely sensing the water status of plants and the water content of canopies remain long-term goals of remote sensing research [1]. Estimates of canopy water content commonly involve measurements in the 900nm to 2000nm portion of the optical spectrum [1]. We have used optical polarization techniques to remove leaf surface reflection and to demonstrate that the visible light reflected by the interior of green healthy corn leaves measured in situ inversely depends upon the leaf relative water content (RWC) [2]. In the research reported here, we again used optical polarization techniques in order to remove the leaf surface reflection from our measurements. This allowed us to monitor the interiors of detached corn leaf samples during leaf dry down measuring for each sample the RWC, bidirectional spectral reflectance and bidirectional spectral transmittance over the wavelength range 450nm to 2,500nm. Our new results like our earlier results show light scattered by the leaf interior measured in the visible wavelength region generally increased as leaf RWC decreased. However, the spectral character and the much improved signal noise of our new results shows the RWC-linked visible light scattering changes are due to leaf structural changes. Our new results show that scattering changes that occur with changing leaf RWC are not attributable to molecular configuration changes in cellular pigments.

Corn↗

Is There Ecological Information in Optical Polarization Data?

Optical linear polarization? In remote sensing it's due to specular reflection. The first surface that incident light encounters - a smooth water surface or the waxy first surface of a leaf's cuticle, if it's even somewhat smooth (i.e. shiny) - will specularly reflect and linearly polarize the incident light. We provide three examples of the types of ecological information contained in remotely sensed optical linear polarization measurements. Remove the surface reflection to better see the interior. The linearly polarized light reflected by leaf surfaces contains no information about cellular pigments, metabolites, or water contained in the leaf interiors of a plant canopy, because it never enters the leaf interior to interact with them. Thus, for purposes of remotely sensing the leaf interiors of a plant canopy, the linearly polarized light should be subtracted from the total reflected light, because including it would add noise to the measurement. In particular 'minus specular' vegetation indices should allow improved monitoring of a plant canopy's physiological processes. Estimate plant development stage and yield. Wheat and sorghum grain heads, following emergence, rapidly extend upward and very quickly tower over nearby leaves, partially blocking our view of the sunlight reflected by those leaf surfaces. The resulting decrease in the amount of surface reflected and polarized sunlight, if monitored over time, potentially allows per-field estimates of the dates of the heading and flowering development stages to be interleaved with weather data in models, which is key to better estimating per-field grain yield. Similar polarization changes may occur in other grasses, such as oats, barley, corn and rice, each a crop so widely grown that it potentially affects climate at the regional scale. Wetlands Mapping. The sunlight specularly reflected by surface waters is blindingly bright, spectrally flat and polarized - all of which telegraphs that the ground area is inundated. Inundated soils exchange methane with the atmosphere; non-inundated soils, carbon dioxide. Aquatic plants growing through the water surface pipe the soil-produced methane via the stomata to the atmosphere, enhancing exchanges rates by factors of 10-20 compared to ebullition (bubbling) or diffusion through the water column to the atmosphere. Thus, mapping wetland areas into three community types - inundated areas with emergent vegetation, open water and uplands - provides potentially key information to water, carbon and energy budgets at landscape to global scales.

Water↗

Optical Polarization of Light from a Sorghum Canopy Measured Under Both a Clear and an Overcast Sky

Introduction: We tested the hypothesis that the optical polarization of the light reflected by a sorghum canopy is due to a Fresnel-type redirection, by sorghum leaf surfaces, of light from an unpolarized light source, the sun or overcast sky, toward the measuring sensor. If it can be shown that the source of the polarization of the light scattered by the sorghum canopy is a first surface, Fresnel-type reflection, then removing this surface reflected light from measurements of canopy reflectance presumably would allow better insight into the biochemical processes such as photosynthesis and metabolism that occur in the interiors of sorghum canopy leaves. Methods: We constructed a tower 5.9m tall in the center of a homogenous sorghum field. We equipped two Barnes MMR radiometers with polarization analyzers on the number 1, 3 and 7 Landsat TM wavelength bands. Positioning the radiometers atop the tower, we collected radiance data in 44 view directions on two days, one day with an overcast sky and the other, clear and sunlit. From the radiance data we calculated the linear polarization of the reflected light for each radiometer wavelength channel and view direction. Results and Discussion: Our experimental results support our hypothesis, showing that the amplitude of the linearly polarized portion of the light reflected by the sorghum canopy varied dramatically with view azimuth direction under a point source, the sun, but the amplitude varied little with view azimuth direction under the hemispherical source, the overcast sky. Under the clear sky, the angle of polarization depended upon the angle of incidence of the sunlight on the leaf, while under the overcast sky the angle of polarization depended upon the zenith view angle. These results support a polarized radiation transport model of the canopy that is based upon a first surface, Fresnel reflection from leaves in the sorghum canopy.

cloud cover↗

Stress-Dependent Voltage Offsets From Polymer Insulators Used in Rock Mechanics and Material Testing

Dielectric insulators are used in a variety of laboratory settings when performing experiments in rock mechanics, petrology, and electromagnetic studies of rocks in the fields of geophysics,material science, and civil engineering. These components may be used to electrically isolate geological samples from the experimental equipment, to perform a mechanical compliance function between brittle samples and the loading equipment, to match ultrasonic transducers, or perform other functions. In manyexperimental configurations the insulators bear the full brunt of force applied to the sample but do not need to withstand high voltages, therefore the insulators are often thin sheets of mechanically tough polymers. From an instrument perspective, transduction from various types of mechanical perturbation has beenqualitatively compared for a number of polymers [1, 2] and these error sources are readily apparent duringhigh-impedance measurements if not mitigated. However even when following best practices, a force dependent voltage signal still remains and its behavior is explored in this presentation. In this experimenttwo thin sheets (0.25 mm) of high-density polyethylene (HDPE) were set up in a stack, held alternatelybetween three aluminum bars; this stack was placed on the platen of a 60T capacity hydraulic testingmachine. The surface area, A, over which the force is applied to the PE sheets in this sandwich is roughly 40 square cm, each sheet forming a parallel-plate capacitor having roughly 320 pF [3], assuming therelative dielectric permittivity of PE is approximately 2.3. The outer two aluminum bars were connected to the LO input ofthe electrometer and the central aluminum bar was connected to the HI input of a Keithley model 617 electrometer. Once the stack is mechanically well-seated with no air gaps, the voltage offset is observed tobe a linear function of the baseline voltage for a given change in applied force. For a periodically appliedforce of 66.7 kN the voltage offsets were measured as a function of initial voltage, and these data were fitwith a linear function that was constrained to pass through the origin. The best fit solution had a correlation coefficient of R=0.85 and a slope of approximately -0.0228 volts/volt. The voltage offset when normalizedis demonstrated to be constant -2.28% for both positive and negative polarities over nearly 3 orders ofbaseline voltage magnitude. From this, the voltage-force coefficient is derived to be -0.34 ppm/N. Thiscorrelates well to a first-order parallel plate capacitor model that assumes constant area, and smalldeformation such that the polymer may be mechanically modeled by a spring that obeys Hookes law. Thissimple model predicts that the coefficient of proportionality is a function of Youngs modulus E= 0.8 GPaand surface area of the insulator, theoretically -1EA= -0.31 ppm/N. The outcome of this work is animproved insulator made from ultra-high molecular weight (UHMW) polyethylene and other approachestoward the minimization of and compensation for these experimental artifacts.

Electromagnetic Studies of Rocks↗