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Datta, N.

Publications and source records attributed to Datta, N..

WO{sub 3} thin film based multiple sensor array for electronic nose application

Multiple sensor array comprising 16 x 2 sensing elements were realized using RF sputtered WO{sub 3} thin films. The sensor films were modified with a thin layer of sensitizers namely Au, Ni, Cu, Al, Pd, Ti, Pt. The resulting sensor array were tested for their response towards different gases namely H{sub 2}S, NH{sub 3}, NO and C{sub 2}H{sub 5}OH. The sensor response values measured from the response curves indicates that the sensor array generates a unique signature pattern (bar chart) for the gases. The sensor response values can be used to get both qualitative and quantitative information about the gas.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Spermine stimulation of a nuclear NII kinase from pea plumules and its role in the phosphorylation of a nuclear polypeptide

We have previously demonstrated that spermine stimulates the phosphorylation of a 47 kilodalton nuclear polypeptide from pea plumules (N Datta, LK Hardison, SJ Roux 1986 Plant Physiol 82: 681-684). In this paper we report that spermine stimulates the activity of a cyclic AMP independent casein kinase, partially purified from a chromatin fraction of pea plumule nuclei. This effect of spermine was substrate specific; i.e. with casein as substrate, spermine stimulated the kinase activity, and with phosvitin as substrate, spermine completely inhibited the activity. The stimulation by spermine of the casein kinase was, in part, due to the lowering of the Mg2+ requirement of the kinase. Heparin could partially inhibit this casein kinase activity and spermine completely overcame this inhibition. By further purification of the casein kinase extract on high performance liquid chromatography, we fractionated it into an NI and an NII kinase. Spermine stimulated the NII kinase by 5- to 6-fold but had no effect on the NI kinase. Using [gamma-32P]GTP, we have shown that spermine promotes the phosphorylation of the 47 kilodalton polypeptide(s) in isolated nuclei, at least in part by stimulating an NII kinase.

NASA Discipline Plant Biology↗

Role of calcium ions in phytochrome responses: an update

Recent findings related to the role of calcium ions in phytochrome responses are reviewed and summarized. Hypotheses tested are the activation of calmodulin by light-regulated Ca2+ transport in cells and the photoinduction of calmodulin-activated enzyme activities. Discussion focuses on evidence that Ca2+ helps to regulate phytochrome responses, calcium requirements for photoinduced spore germination in the fern Onoclea, Ca2+ fluxes and phytochrome function in the alga Mougeotia, calmodulin antagonist blocking of red-light stimulated chloroplast rotation, the role of phosphorylation in calmodulin-regulated responses, and phytochrome regulation of nuclear protein phosphorylation.

NASA Discipline Plant Biology↗