Engineering topics
Deacon, A. M.
Publications and source records attributed to Deacon, A. M..
Structure and Function of a Novel LD-carboxypeptidase A Involved in Peptidoglycan Recycling
Abstract Not Provided
Branched Signal Wiring of an Essential Bacterial Cell-Cycle Phosphotransfer Protein
Abstract Not Provided
Genomics, Evolution, and Crystal Structure of a New Family of Bacterial Spore Kinases
Abstract Not Provided
Structure of a Virulence Regulatory Factor Cvfb Reveals a Novel Winged Helix RNA Binding Module
Abstract Not Provided
Finding a Cold Needle in a Warm Haystack: Infrared Imaging Applied to Locating Cryocooled Crystals in Loops
We demonstrate the use of inbred imaging to locate crystals mounted in cryoloops and cryopreserved in a nitrogen gas stream at 100K. In the home laboratory crystals are clearly seen in the infrared images with light transmitting through the sample while irradiating the crystal from behind, and with illumination from a direction perpendicular to the direction of view. The crystals transmit and reflect infrared radiation differently from the surrounding mother liquor and loop. Because of differences in contrast between crystals and their surrounding mother liquor, it is possible to clearly identify the crystal position. In use at the synchrotron, with robotically mounted crystals the small depth of field of the lens required the recording of multiple images at different focal points. Image processing techniques were then used to produce a clear image of the crystal. The resulting infrared images and intensity profiles show that infrared imaging can be a powerful complement to visual imaging in locating crystals in cryocooled loops.