Complete sequences of conjugal helper plasmids pRK2013 and pEVS104
We present the complete sequences of two commonly used conjugal helper plasmids: pRK2013 and pEVS104. These sequences will enable engineering of custom helper plasmids, for example, with different antibiotic markers or origins of replication. We provide both sequence information and plasmid maps to aid future engineering efforts.
59 BASIC BIOLOGICAL SCIENCES↗