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Engineering topics

Ellis, S.

Publications and source records attributed to Ellis, S..

At least 37 records · Page 2

Plasma vasopressin and renin activity in women exposed to bed rest and +G/z/ acceleration

To study the effect of prolonged recumbency on plasma vasopressin and renin activity, eight women were subjected to 17 days of absolute bed rest. The tolerance to +3G vertical acceleration of the subjects was tested before and after 14 days of bed rest. From day 2 and through day 17 of bed rest, plasma arginine vasopressin (AVP) levels were reduced 33%. Plasma renin activity (PRA) increased 91% above ambulatory control values from days 10 through 15 of bed rest. When compared to precentrifuge values, exposure to vertical acceleration prior to bed rest provoked a 20-fold rise in mean plasma AVP but resulted in only a slight increase in PRA. After bed rest, acceleration increased plasma AVP 7-fold; however, the magnitude of this increase was less than the post +3G acceleration value obtained prior to bed rest. After bed rest, no significant rise was noted in PRA following +3G acceleration. This study demonstrates that prolonged bed rest leads to a significant rise in the PRA of female subjects, while exposure to positive vertical acceleration provokes a marked rise in plasma AVP.

Keil, L. C.↗

Studies on the nature of plasma growth hormone

The paper presents further evidence for the existence of two discrete forms of growth hormone in human plasma, one which is detectable by both radioimmunoassay and bioassay and is immunoreactive, and the other, termed 'bioactive', which is detected by tibial bioassay but shows little reactivity with currently available antisera to pituitary growth hormone. The same division of immunoactive and bioactive growth hormone occurs in rats, though with less disparity. Tests on rats indicated that the bioactive hormone is preferentially released into jugular vein plasma and that plasma concentrations of the bioactive hormone can be enhanced by insulin administration. The bioactive hormone was detectable by tibial assays in Cohn fractions IV, IV-1, and IV-4, and could be concentrated about 40-fold by fractionation with (NaPO3)6 and (NH4)2SO4.

Ellis, S.↗

Studies on human urinary arylamidases

Human urinary protein was found to contain enzymes that hydrolyze leucyl-, alanyl-, and glycyl-prolyl-beta-naphthylamides. The kinetic constants of these enzymes were determined and their chemical properties studied. The pH optima for the hydrolysis of the various naphthylamides were also determined. Glycyl-prolyl-arylaminade was inhibited by Co(2+) and Mn(2+), while two other arylamidases were slightly activated by Co(2+). p-Chloromercuriphenyl-sulfonate and puromycin significantly inhibited leucyl and alanyl arylamidases. The mean values for 24-hour urinary output for leucyl-, alanyl-, and glycyl-prolyl arylamidases in normal human male subjects were 4.32, 9.97, and 2.2 units, respectively.

Raina, P. N.↗

Induction of chronic growth hormone deficiency by anti-GH serum

The observations reported indicate that the growth rate of neonatal rats can be specifically inhibited for at least 78 days following the administration of antisera against growth hormone (GH) for only four days after birth. The inhibition can be correlated with a marked deficit of tibial growth promoting activity in the pituitary but not with the plasma concentrations of immuno-reactive GH.

Grindeland, R. E.↗

Detection of a lysosomal carboxypeptidase and a lysosomal dipeptidase in highly-purified dipeptidyl aminopeptidase I /cathepsin C/ and the elimination of their activities from preparations used to sequence peptides.

Description of the properties of a carboxypeptidase, termed 'catheptic carboxypeptidase C,' and a dipeptidase, termed 'Ser-Met dipeptidase.' Both were found in highly purified DAP I from either beef spleen or rat liver. Methods are described for the removal or selective inactivation of these contaminating enzymes.

Mcdonald, J. K.↗