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Fontana, Pietro

Publications and source records attributed to Fontana, Pietro.

6 records
All sourcesNASA NTRSDOE OSTI
DOE OSTI2022

Structure of cytoplasmic ring of nuclear pore complex by integrative cryo-EM and AlphaFold

The nuclear pore complex (NPC) is the conduit for bidirectional cargo traffic between the cytoplasm and the nucleus. We determined a near-complete structure of the cytoplasmic ring of the NPC from Xenopus oocytes using single-particle cryo–electron microscopy and AlphaFold prediction. Structures of nucleoporins were predicted with AlphaFold and fit into the medium-resolution map by using the prominent secondary structural density as a guide. Certain molecular interactions were further built or confirmed by complex prediction by using AlphaFold. Here, we identified the binding modes of five copies of Nup358, the largest NPC subunit with Phe-Gly repeats for cargo transport, and predicted it to contain a coiled-coil domain that may provide avidity to assist its role as a nucleation center for NPC formation under certain conditions

59 BASIC BIOLOGICAL SCIENCES↗