Recombinant host cells for the production of malonate
Systems and methods for the production of malonate in recombinant host cells.
Dietrich, Jeffrey A.↗
Engineering topics
Fortman, Jeffrey L.
Publications and source records attributed to Fortman, Jeffrey L..
Recombinant host cells for the production of malonate
Systems and methods for the production of malonate in recombinant host cells.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Isolation and Characterization of Bacterial Cellulase Producers for Biomass Deconstruction: A Microbiology Laboratory Course.
The conversion of biomass to biofuels presents a solution to one of the largest global challenges of our era, climate change. A critical part of this pipeline is the process of breaking down cellulosic sugars from plant matter to be used by microbes containing biosynthetic pathways that produce biofuels or bioproducts. In this inquiry-based course, students complete a research project that isolates cellulase-producing bacteria from samples collected from the environment. After obtaining isolates, the students characterize the production of cellulases. Students then amplify and sequence the 16S rRNA genes of confirmed cellulase producers and use bioinformatic methods to identify the bacterial isolates. Throughout the course, students learn about the process of generating biofuels and bioproducts through the deconstruction of cellulosic biomass to form monosaccharides from the biopolymers in plant matter. The program relies heavily on active learning and enables students to connect microbiology with issues of sustainability. In addition, it provides exposure to basic microbiology, molecular biology, and biotechnology laboratory techniques and concepts. The described activity was initially developed for the Introductory College Level Experience in Microbiology (iCLEM) program, a research-based immersive laboratory course at the US Department of Energy Joint BioEnergy Institute. Originally designed as an accelerated program for high-potential, low-income, high school students (11th–12th grade), this curriculum could also be implemented for undergraduate coursework in a research-intensive laboratory course at a two- or four-year college or university.
59 BASIC BIOLOGICAL SCIENCES↗
Host cells and methods for producing diacid compounds
The present invention provides for a method of producing one or more fatty acid derived dicarboxylic acids in a genetically modified host cell which does not naturally produce the one or more derived fatty acid derived dicarboxylic acids. The invention provides for the biosynthesis of dicarboxylic acid ranging in length from C3 to C26. The host cell can be further modified to increase fatty acid production or export of the desired fatty acid derived compound, and/or decrease fatty acid storage or metabolism.
59 BASIC BIOLOGICAL SCIENCES↗
Producing alpha-olefins using polyketide synthases
The present invention provides for a polyketide synthase (PKS) capable of synthesizing an .alpha.-olefin, such as 1-hexene or butadiene. The present invention also provides for a host cell comprising the PKS and when cultured produces the .alpha.-olefin.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Recombinant host cells for the production of malonate
Systems and methods for the production of malonate in recombinant host cells.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound
The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phoshpate and/or ribulose 5-phospate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.
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