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Gioia D Massa

Publications and source records attributed to Gioia D Massa.

At least 19 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS2025

The Microbiome of A Tomato Crop Grown Under Different Lighting Regimes on the International Space Station

The VEG-05 experiment presented here investigated the effect of red-rich and blue-rich light recipes in Veggie on the microbiome of the Veggie facility and the plant tissues of a dwarf tomato variety, Solanum lycopersicum cv. Red Robin. For food safety, the plants were screened using culture-based methods for potential human pathogens that may cause infection by consumption of the fruit. The microbiome was investigated using bacterial 16S and fungal ITS sequencing methods to enumerate and identify bacterial and fungal communities on tomato fruit, roots, leaves, rooting substrate, and Veggie facility surfaces grown under blue-rich or red-rich lighting. Comparisons of microbial communities were made between lighting treatments, as well as for flight and ground controls. This analysis determined the core microbiome and microbiological composition for tomato plants grown under a blue-rich or red-rich lighting treatment and microgravity conditions. Culture-based pathogen screening, corroborated by 16S and ITS sequencing, yielded negative results. Bacterial and fungal counts were lower for ground controls than in-flight samples. However, there were no differences in microbial counts between lighting treatments. Regardless of lighting treatment, plant components shared a core microbiome, although some differences were observed in genera between lighting treatments.

Veggie↗
NASA NTRS2024

Pick-and-Eat Space Crop Production Flight Testing on the International Space Station

Fresh, nutritious, palatable produce for crew consumption on long-duration spaceflight missions may provide health-promoting, bioavailable nutrients and enhance the dietary experience. VEG-04A and VEG-04B explored growing leafy greens on the International Space Station using the Veggie Vegetable Production System. Two flight tests with ground controls were conducted in 2019 growing mizuna mustard, where Veggie chambers were set to different red-to-blue-to-green light formulations. Light quality affects plant growth, nutrition, microbiology, and organoleptic characteristics on Earth, and we examined how these vary in microgravity and under different harvest scenarios. Astronauts harvested and weighed mizuna and completed organoleptic evaluations. Flight samples were returned to Earth for nutritional quality and microbial food safety analyses. Yield and chemistry differed between ground and flight samples and light treatments, and bacterial and fungal counts were lower in ground than in flight samples. This research helps increase our understanding of the requirements for growing high-quality crops in spaceflight.

Food Safety, International Space Station, Nutritio↗
NASA NTRS2021

Microbial Food Safety in Space Production Systems

While traveling to deep space is difficult for many reasons, food is a crucial one. Round-trip Mars mission scenarios last 3 years, demanding food with a shelf-life of 5 years; this means that feeding human crew sustainably for long-duration missions beyond low Earth orbit (LEO) will ultimately lead to a paradigm shift away from the current Earth-based food production system, which depends upon storing and transporting prepackaged foods, and toward bio-regenerative production of food in space. Pharmaceuticals and nutritional supplements face similar challenges. Moreover, the methods we currently use to detect dangerous microbes in food require sample return to Earth, a situation not viable for deep-space missions. While the science behind generating foods and bioproducts is covered by other white papers, in this paper we discuss a crucial gap uniting all of them: how to ensure that such products are free of unwanted microbial contamination and safe for crew to consume. Because Earth-based food safety systems cannot be directly applied in space, safety assurance is currently a critical bottleneck in the space production of food and other bioproducts. Future sustainable deep-space missions will require NASA to devote more resources in the coming decade to understanding the biological and physical science principles underlying microbial food safety in space, and to developing efficient, reliable methods in this area.

microbiology↗
NASA NTRS2021

Gaps List KSC Space Crop Production Project Scientist: Interview Evaluation

The Space Crop Production (SCP) at the NASA Kennedy Space Center (KSC) is aimed at achieving nutrient supplementation and moving towards caloric independence from Earth by growing crops for astronauts. However, growing crops outside of Earth’s orbit creates many new challenges. The KCS SCP Project Scientists have developed a method of organizing these challenges, called the Gaps List. This Gaps List is a dynamic, taxonomically arranged list of missing knowledge and technologies needed to reach production goals. The purpose of this evaluation was to assess the effectiveness and current research coverage of the Gaps List by using it to document the KSC SCP Program research. In evaluating the research, it was determined that several needs within the SCP Program that are not being met, and many deficiencies within the Gaps List were encountered. Gaps involving hardware were largely unrecognized by the KSC researchers and the funding sources that would typically support them. The KSC SCP Project will require more engineering and physical sciences support to close those gaps. The Gaps List was found to be out of date and in need of rearrangement. A recommendation from this review was that the Gaps List be reassessed at regular intervals to ensure all gaps and relevant research topics are included. In order to better understand the gaps within the list, any assessment needs to include a measure of priority, dependency and breadth. These measures may be included within or along side the current taxonomical arrangement of the Gaps List.

Space Crop Production↗
NASA NTRS2020

Development of a Photosynthesis Measurement Chamber under Different Airspeeds for Applications in Future Space Crop-Production Facilities

Space crop production systems are being developed to grow fresh produce in-situ to supplement the astronauts’ diet, but the required ventilation rates for crops in different gravity environments remains poorly understood. The reduction or lack of buoyancy-driven convection in reduced gravity environments leads to impaired gas exchange (CO2 absorption, water transpiration and O2 release) at the leaf surface if no extra ventilation is provided, and this could lead to a reduction in biomass production in the long run. To better characterize the influence of different airspeeds on photosynthesis and be able to model this in low gravity, a chamber was designed to interface with a LI-6800 portable photosynthesis system. This paper details the design of this chamber, specifically made to measure whole-plant and small canopy gas exchange at different airspeeds. The fans provide turbulent mixing in the chamber to ensure that it behaves like a continuous stirred tank reactor (CSTR)and that the residence time distribution (RTD) is the same for any fan speed; the computational fluid dynamic (CFD) model of the gas domain (the air in the chamber) hence uses a k-omega turbulence model. An airflow map of the chamber was created using anemometer measurements for the different airspeeds tested, and this was used together with the CFD simulation results to relate the experimentally measured fan outputs to actual airspeeds on top of an artificial plant. Environmental parameters (air temperature, relative humidity, CO2level) are controlled by the LI-6800. This work was funded by NASA Space Biology through the NASA postdoctoral program / USRA.

Lucie Poulet↗
NASA NTRSDate not supplied

Understanding the Impacts of Deep Space Environment on Crop Production

NASA’s goal of developing sustainable habitats to support long duration, deep space missions requires advanced science, technology, and engineering. Understanding the integrated, long-term effects of deep space environments on biological systems is needed. Sustainable habitats require the production of food and oxygen on site.

Bruce M Link↗
NASA NTRSDate not supplied

Microbiological Analysis of Mizuna Grown in the Veggie Hardware to Define Critical Control Points and Ensure the Safety of Space Grown Crops

The Veggie facility on the International Space Station has been utilized as a “pick and eat” plant growth system to provide fresh produce for crew consumption. The VEG-04 experiments completed in 2019 examined the effect of red-rich and blue-rich light treatments on the growth of mizuna as well as harvest method and resulting yield. Analysis was performed on plant tissues and associated hardware to evaluate the influence of these experimental variables on the microbial population. VEG-04A plant pillows with pre-planted Mizuna mustard seeds were launched on SpaceX-16 in December 2018. The pillows were initiated, and a single 35-day harvest was performed. Veg 04B pillows were pre-planted with Mizuna seeds and launched on SpaceX-18 in July 2019, initiated and subsequently harvested at days 29, 43 and 58. The crew consumed approximately half of the produce, and the remainder was frozen and returned for analysis. Leaves, swabs, wicking material, substrate, and roots were processed and plated on media for the enumeration and isolation of bacteria and fungi. Isolated bacterial colonies were identified using Biolog Micro ID system or MicroSEQ16S rDNA sequencing technique. Fungal colonies were identified using the MicroSEQ D2 rDNA kit. Sample extracts were plated onto specialized media to identify Escherichia coli/coliforms, Staphylococcus aureus and Salmonella sp. Results indicate that bacterial and fungal counts were higher in plants grown in red-rich lightin VEG-04A, while the opposite was true in the Veg-04B third harvest. Microbial counts increased with the repeated harvest method used in Veg-04B. These data support the understanding of environmental and horticultural practices that can affect the microbiological quality of space-grown produce grown and aid in identification of critical control points for the development of a hazard analysis critical control point plan for ISS-grown crops. This research was co-funded by the NASA’s Human Research Program and Space Biology.

Mary E Hummerick↗
NASA NTRSDate not supplied

Seed surface sanitization and persistence of E.coli through different tissues of ‘Red Robin’ Tomato (Solanum lycopersicum cv. Red Robin)

Seed surface sanitization via chemical processes removes/reduces microbes from the external surfaces of the seed and thereby could have an impact on the plants’ health or productivity. To determine the impact of seed surface sanitization on the plants’ microbiome, sanitized and unsanitized seeds from ‘Red Robin’ Tomato (Solanumlycopersicum cv. Red Robin) were exposed to Escherichia coli (E. coli) and grown in a controlled environment growth chamber simulating environmental conditions aboard the International Space Station (ISS). Plants were harvested at four intervals, days 11, 33,42 and 76 post-germination. Changes in the microbial communities of leaf, stem, root, and fruit because of E. coli exposure and the persistence of E. coli itself were investigated using aerobic plate count (APC), qPCR and 16S rRNA sequencing. It was determined that E. coli persisted for longer periods of time in plants from sanitized versus unsanitized seeds and was identified in root tissue more frequently than in leaf or stem tissue. E. coli was not detected in fruits raised from either sanitized or unsanitized seeds. The 16S rRNA sequencing showed dynamic changes in the abundance of members of the phylum Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes in all tissue types studied. We observed minimal or no changes in the alpha diversity of leaf stem and fruit tissue with time, or between sanitized and unsanitized seeds. Roots showed significant differences in alpha diversity with time and seed sanitization status. Beta-diversity showed that time had more of an influence on all samples versus the E. coli treatment. Members of phyla Proteobacteria and Bacteroidetes were found to be differentially abundant across leaf, stem and root tissue. Our results indicated that the seed surface sanitization, although a requirement for sending seeds to space, might influence the developing microbiome. This research was funded by NASA’s Space Life and Physical Sciences Research and Applications.

Anirudha R Dixit↗
NASA NTRSDate not supplied

Selection Factors for Space Crops

NASA is actively researching space crop production to determine its potential to contribute to food system security on long duration missions beyond Low Earth Orbit. Our near-term focus is on nutrient and variety supplementation of prepackaged food with fresh produce that requires little or no processing. The longer-term goal is caloric replacement to become less dependent on Earth, and this will require cultivation of staple crops, processing and cooking equipment, integration with spacecraft air, water, and power systems, and automation. There are numerous technology and knowledge gaps remaining for sustainable space crop production systems, but one high-impact area is in the development of crops specifically customized to meet the needs of controlled environment crop production, astronaut health and well-being, and space-unique environments. Modern crop breeding and genome engineering tools are allowing for rapid development of new genotypes with incredible specificity. Targeted aspects to optimize crops for space have been identified and characterized into five categories: plant growth and development, plant physiology, produce nutrition, produce organoleptic acceptability, and postharvest characteristics. Within each category there are several targets that further the development of crop production systems for spaceflight, such as crop size and harvest index, tolerance to specific environmental stresses, optimizing target nutrients that are low or degrade in the packaged diet, maintenance time requirements, and less indigestible structural material. NASA-funded PIs are already beginning to develop candidate crops, and spaceflight testing and validation of novel space crops is on the horizon. Crops developed for space also have the potential to benefit terrestrial controlled environment agriculture crop production systems. This research was supported by NASA’s Space Biology and Human Research Programs.

Space Crop Production↗
NASA NTRSDate not supplied

Documentarian’s Support Role: Recording Active Research for Outreach

The Space Crop Production Team at NASA’s Kennedy Space Center recently conducted a campaign of parabolic flights to study the best techniques for harvesting and collecting microgreens in microgravity. During this process, the research team invited a student with scientific training to the field sites, contributing as the documentarian and aiding in recording audio visual data. The documentarian was tasked with photographing and filming the preparation of experiments for parabolic flight, hardware installation in the parabolic flight plane, the crew performing their experiments, and the harvesting hardware and samples post-flight. Through having a documentarian on the team during experiments, the processes of problem-solving, teamwork, and data collection were able to be captured. This allows for these methods to be reflected upon post-experiment as a means of improving the procedures for the future. Additionally, the picture and video evidence became data that were analyzed following the flight test. The Space Crop Production team benefited by having a documentarian present, as they were able to develop a 45 second social media video with the intent of being published through official NASA outlets. Longer videos for classroom use are also in work. In addition to outreach usage, the collection of footage and visual data the documentarian provides has the potential to be scientifically published both in video-based scientific journals, and as supplemental data in online journal publications. Capturing research in this manner helps share an unseen side of the research practices with a larger audience and increases the potential for scientific engagement and activation.

Christopher Bermudez↗
NASA NTRSDate not supplied

Harvesting Microgreens in Microgravity: Analysis of Six Different Methods

In long duration space missions, crops will be used to supplement the astronaut diet. One such proposed crop type is microgreens, the young seedlings of edible plants that are known for their high nutritional levels, intense flavors, colorful appearance, and variety of textures. While these characteristics make microgreens a great candidate for space crop production, their small size presents a unique challenge within the microgravity environment. To ensure that astronauts will be able to harvest microgreens in microgravity with ease while avoiding the introduction of debris to the spacecraft cabin, multiple harvesting methods were developed by the Space Crop Production Team at NASA’s Kennedy Space Center. Three parabolic flights were conducted in November and December of 2021 and during those flights three different microgreen cutting methods (guillotine, pepper grinder, scissors) as well as two different bagging methods (attached and manual) were tested. In each flight, the microgreens were contained inside of a glovebox and footage of all the microgreen harvests was recorded. The cutting and bagging method combination that introduced the lowest average number of particulates into the glovebox was the scissors with attached bagging, closely followed by the pepper grinder with attached bagging. However, the scissors with attached bagging may had introduced fewer particulates into the glovebox because on average, 27% of the microgreens were never cut using the scissors method, so there were fewer free-floating particulates generated. The cutting and bagging method combination that left the lowest average percentage of microgreens remaining on the hardware post-harvest was the pepper grinder with an attached bag. Future directions include involving microgreen harvests in analog environments and further development of the different microgreen cutting and bagging methods. This research was funded by multiple NASA grants at the Kennedy Space Center.

Haley O Boles↗
NASA NTRSDate not supplied

RGB Imaging as a Tool to Monitor Indoor Crop Plant Production

Future crop production in space will require robust monitoring technologies that can optimize crop yield, reduce waste, and generate data for an automated plant growth design. Imaging has been suggested as a tool for measuring plant health, yet imaging systems for indoor crops have not been tested in spaceflight. Fortunately, RGB images of crop plants growing inside the Advanced Plant Habitat (APH) aboard the ISS have already been captured. In ground-based studies, the Kennedy Space Center (NASA, KSC) is collaborating with the United States Department of Agriculture (USDA ARS) to develop an imaging system for monitoring indoor crop plant health. In one study, we applied a drought stress to ‘Dragoon’ lettuce plants over a period of 14 days and captured RGB images in 24 h increments. Images were analyzed, and by applying a difference index, the images were able to be used to detect the drought stress in lettuce. This difference index was then applied to RGB images collected inside the APH ground unit for a pre-flight experiment growing ‘Outredgous’ lettuce under different substrate moisture conditions, and results showed that the RGB camera was capable of detecting drought stress inside the spaceflight plant growth hardware. These results suggest that RGB cameras already deployed to space may offer valuable information for monitoring plant production in extraterrestrial environments. This research was supported by NASA’s space biology program.

Rachel Tucker↗