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Gioia Massa

Publications and source records attributed to Gioia Massa.

At least 19 records

3D Printed Materials Characterization for Rapid Prototyping and Plant Growth

Through KSC IRTD funding in 2022, this project brought a list of 18, 3D printed filaments into formal characterization testing to provide a reference for their behaviors under relevant applications. The project format set up a series of tests to expose 3D printed specimens. A total of 1,989 individual 3D printed test specimens were sent across KSC to be scrutinized by three laboratories to fulfill a multidisciplinary assessment of each material TRL. Testing started with 18 materials. Initially, seed germination assays in the PPA, sample materials were enclosed in petri dishes with lettuce seeds on damp germination paper. No significant impacts on lettuce seed germination were observed in this testing. Next, sample coupons were printed and sent for materials testing to the KSC Analysis/Mechanical and Environmental Testing Laboratory, where they were subjected to 14- and 30-day soak periods in solutions used to provide nutrients to plants or to sanitize hardware before and after use. Following a long soak typical of a 30-day plant growout in Hoagland’s solution, 14 materials gained more than 10% of their own mass. This indicated an increased potential for leaching or providing conditions that are not food safe. Materials that exceeded 15% absorption by mass were eliminated from further testing. Based off this result, the team continued with a core list of nine filaments to fulfill Tensile, Flexural, Biofilm formation, and plant growth testing. Those materials were PLA (Raise3D), ABS (Raise3D), PETG (PolyethyleneTerephthalate Glycol) (Raise3D), ASA (Acrylonitrile Styrene Acrylate) (Raise3D), PC (Polycarbonate) (Raise3D), TPU (Thermoplastic polyurethane)-95 (Raise3D), PLA Copper (Gizmodorks), PP (Polypropylene) (Braskem), and HIPS (High Impact Polystyrene) (Gizmodorks). Testing also quantified the spectral impact of using different color 3D printed surfaces in a growth chamber. The material used for spectral testing was PLA. Printing employed a standard surface texture representative of all materials. It was shown through Tensile Testing (ASTM D638-22) that the breaking force of a 3D printed part greatly varied depending on layer orientation. This is common through all materials, and demonstrates that the strength of a 3D printed component can be maximized by layering the material normal to the primary force on the part. Four-point flexural testing (ASTM D790) provided quantities of interest, Flexural modulus, Flexural strength, Flexural stress, and strain at break within a 5% strain limit from each of nine materials. Biofilm formation testing was conducted in the Molecular and Microbiological Laboratory. Testing completed on specimens from each material showed equal formation on the surface. Additional plant growth testing was conducted in the PPA beyond the initial germination testing. The final assessment documents that three materials (PLA, ABS, and PC) have reached TRL 6 through extensive testing, and ultimate end-to-end applied use in experimental or testing conditions (flight and ground). TRL 5 materials (ASA, TPU-95, PLA Copper, PP, PETG, and HIPS) have all been successfully applied in Research and Development for crop growth applications and are ready to be applied in formal testing. TRL 4 materials Nylon910, PLA Carbon Fiber, PPA CF, PPA Glass Fiber (GF), NinjaFlex, and P-filament 721 are materials that were able to be printed and tested, but have yet to show data meeting applied requirements. TRL 3 NylonX, Flex TPE-185, and Nylon were unable to be reliably printed to fulfill testing. These results provide researchers with reference for materials to use during plant growth experimentation, and also set a standard for future characterization work applying 3D printing and materials to testing, research, and experimentation.

Gioia Massa↗

Plants in Space and Space Crop Production

As astronauts venture farther from Earth, and for longer periods, the space food system will increase in importance. Crop production can supplement a packaged diet to provide additional nutrients and dietary variety for astronauts, and in future missions, bioregenerative approaches may be used to generate a larger percentage of the diet. Plants may also provide behavioural health benefits and assist with other life support functions. Several unique challenges exist for growth of plants in microgravity and on other planetary surfaces like the moon and Mars. Testing with the Veggie and Advanced Plant Habitat (APH) chambers on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth, nutritional content, acceptability, and the importance of plants to astronauts living and working away from Earth. We are also gaining a better understanding of food safety concerns and the behaviour of space plant microbiomes and plant pathogens. As we transition from research towards operational space crop production to enable human exploration, there are several gaps and challenges of growing crops in space that must be addressed. Research and technology development in key areas such as water and nutrient delivery, plant health monitoring, and crop selection are needed to overcome these challenges. Additionally, there are opportunities for breeding or engineering of custom space crops related to plant growth and development, plant physiology, produce nutrition, organoleptic acceptability, and post-harvest characteristics. Solutions to help ensure food security off-Earth may also translate to better approaches to terrestrial sustainable crop production.

Advanced Plant Habitat↗

CESO 22-4: Parabolic and Suborbital Glovebox in Support of Space Crop Production

Space crop production research approaches and technologies can be validated for microgravity using parabolic or suborbital flight opportunities, and this will save considerable risk, time, and money for implementing new strategies in spaceflight. While the durations of microgravity in these tests are insufficient to grow crops, there are numerous examples of the value of microgravity testing for subsystems such as plant water and nutrient delivery, where multiphase fluid flow can be elucidated in short durations, and horticultural operations, such as harvesting and produce sanitation where containment and contamination can be assessed. Containment in any operational test is essential, as crop operations involve fluids and biological samples, which are potential hazards. This project consists of designing, developing, and constructing a parabolic/suborbital glovebox for experiment containment. The design involves modifications and upgrades to an existing glovebox developed at the University of Louisville. The University of Louisville glovebox was used by KSC researchers for space crop parabolic flight tests in 2021, and lessons learned from that testing have driven design modifications and improvements in the KSC-generated glovebox. Requirements were identified, parts were ordered, an operational science glovebox was fabricated, and a detailed materials specification list was generated. Analyses that are required for flight, remain to be performed to meet airworthiness requirements, and that work will have to be conducted in the future before use in flight.

Food Production↗

Microgreens Root Zone/Shoot Zone Partitioned Planting Box

To enable sustainable food production in future human exploration missions, plant growth is being studied by the Space Crop Production Team at KSC. Microgreens are good candidates for food supplements and contain specific nutrients that are lacking in the prepackaged diet, including vitamin C and vitamin K. Because they are densely sown, typical growth methods do not allow the ability to distinguish between the levels of evaporation from the rooting substrate and transpiration from the leaves. With larger plants, the root and shoot zone can be separated to distinguish these fluxes and accurate transpiration measurements of plant canopies are feasible. Furthermore, separation of the root and shoot zone may also be beneficial when harvesting microgreens in microgravity as it may reduce microbial contamination of the edible biomass by the roots, which have high microbial loads. Using a root and shoot separator box when harvesting may help with microbial contamination, but these tiny plants are challenging to handle in microgravity, so harvest management remains an open question. The innovation proposed here is a microgreen root/shoot partitioned planting box, which offers a solution to these challenges with accurate gas exchange measurements and a safe microgreen harvest in low gravity environments. Being able to measure transpiration of a microgreen canopy will be important for modeling plant growth in reduced gravity environments, so the first objective was to develop a planting unit with a seal between the root zone and the shoot zone. With an unsuitable harvesting technique, freshly harvested microgreens may add debris to the cabin, so the second objective was to test different harvesting techniques and management approaches associated with this innovation. These two objectives were pursued in parallel since many goals were the same: develop a planting unit that 1) separates the shoots from the roots, 2) allows acceptable germination rate, and 3) allows for seedlings to emerge and develop. What differed was the need to have a seal, which was only applicable for our gas exchange goal, and the need to have an embedded harvesting mechanism and bagging method which only applied to our harvesting goal. Testing of the various harvesting mechanisms and bagging methods was performed during a series of parabolic flights. All parabolic flight procedures took place inside a rented secondary containment chamber (e.g., glovebox) that was developed by the University of Louisville specifically for experiments involving fluids and other materials that may become airborne during reduced gravity flight. Three different harvesting methods and two different bagging collection methods were tested for microgreens. A third bagging method was initially tested but found to be unsuitable. Human factors were also taken into consideration, to identify which harvest and bagging collection methods would be easiest to use with favorable results in microgravity. Three parabolic flight tests were performed in total, one in November 2021 and two in December 2021.

Gioia Massa↗

What are Needed Innovations for Space Crop Production That May Also Benefit Vertical Farming?

I am speaking about the space crop production gaps and challenges we face and how we need innovations in the area of plant health monitoring, food safety contamination monitoring, CEA IPM, understanding of and manipulation of the microbiome/ecosystem, sustainability needs, novel sensors needed, automation and robotics, and also new crops that could be developed and ways that our needs parallel those of the vertical farming industry. (Note: This presentation is in video mp4 format is only viewable by download)

Gioia Massa↗

Growing Plants in Space

A short presentation on space plant growth and the Grow for Launch Earth Day activity for the Maryland Library Association virtual meeting.

Veggie↗

Adding Fresh Produce to the Space Diet

NASA’s Human Research Program has identified a Risk of inadequate nutrition and food stability as a risk of high concern for future long duration exploration missions such as a mission to Mars. One possible solution to help reduce this risk is the addition of in situ produced supplemental fresh produce to supplement and enhance the astronaut diet. A number of ground and ISS-based studies are being conducted which are helping to reduce this risk by filling the gaps in knowledge and technology associated with space crop production. These include studies with the Veggie vegetable production system on the International Space Station. Key focus areas include water and nutrient delivery, food safety and the crop microbiome, crop selection and testing, automation and robotics, radiation impacts, seed storage and handling, and scalability for different concepts and architectures. All of these areas have significant challenges that need to be addressed prior to successful integration of crop production into the crew diet. Data from these studies is feeding the development of new technologies and systems for space crop production. This research was funded by NASA Space Biology and NASA’s Human Research Program.

Gioia Massa↗

Growing Plants In Space

Explore the source record for details and available documents.

Space Crop Production↗

Effects of Supplemental Far-Red Light on Leafy Green Crops for Space

The use of plants to provide food and eventual bioregenerative life support has been studied for nearly 50 years. A logical starting point for early missions like the International Space Station (ISS) is to grow leafy greens to supplement the crew’s diet of packaged foods. In an attempt to expand the list of potential crops, NASA conducted ground studies with eight leafy greens: ‘Dragoon’ lettuce, ‘Extra Dwarf’ pak choi, shungiku, ‘Barese’ Swiss chard, ‘Red Russian’ kale, ‘Toscano’ kale, ‘Amara’ mustard, and ‘Outredgeous’ lettuce, which has been used in prior ground and flight tests with the Veggie Plant Chamber. Plants were grown for 28 days under 320μmol m(exp -2)s(exp -1) PPFD from LED lights, 3000 ppm CO2, and 23 C to simulate an environment similar to the Veggie Plant Chamber aboard ISS. Half of the plants were given ~7 μmol m(exp -2)s(exp -1) and the other half, ~23μmol m(exp -2)s(exp -1) of supplemental far-red (735 nm). Supplemental far-red light resulted in increased fresh mass yields for some species but not all. This could be due to the relative small amount of far-red photons even in the supplemental treatment. ‘Extra Dwarf’ pak choi and ‘Dragoon’ lettuce produced the highest yields (70-80 g FM/plant) under both lighting regimes. A more consistent response to supplemental far-red light was increased plant canopy cover and increased shoot heights, which may be a consideration for volume constrained systems in space.

LaShelle E Spencer↗

Students Planting Research Of Use To Space (SPROUTS) Networking and Engagement of Interns at Kennedy Space Center

While Kennedy Space Center (KSC) has had summer interns in the life sciences for many years, beginning in 2016, the internship program was re-envisioned. Rather than having many interns in the summer with numerous innovative small projects created primarily for this purpose, the current model is to offer internship opportunities every semester (Spring, Summer, and Fall), so that research is continuous and sustained. Since 2016 we have had 64 internship opportunities for undergraduate and graduate students in the areas of space crop production and microgravity simulation support. Some students have participated in more than one opportunity, so 52 separate individuals have participated in this program. While interns primarily worked with individual mentors on a main project, most also worked on group projects with other interns or developed smaller secondary projects on their own initiative. In addition, all interns participated in a weekly Lunch, Learn, and Discuss seminar series where they were encouraged to present their research, learn from scientists at KSC, and engage with members of the space life sciences community. When the face-to-face spring internship of 2020 ended prematurely, we resumed the weekly seminar series as a virtual event, and this led to the concept of engaging former interns. Current and former interns were invited to participate in the on-going virtual series, and 46 asked to be included on the mailing list. Our current seminar series involves remote seminars or hosted discussions from contacts within and outside of the agency, and engages a large network of current scientists and former interns with an weekly participation averaging 40-50 people. Speakers include experts from industry, academia, and other space agencies working on topics of interest to KSC space life science researchers. In addition, this series has served as a brain trust and brainstorming mechanism for KSC scientists to invite and discuss new ideas and concepts. The advent of remote work and virtual platforms has allowed us to engage with interns in a new way that will continue as future interns are integrated into this network of individuals interested in space research. This work is funded by NASA’s Space Biology Program.

Internships↗

Microbial Food Safety for Space Crops

This presentation will highlight the unique challenges and opportunities of microbial food safety for space grown produce as part of a joint webinar on controlled environment food safety.

Veggie↗