Variant Hyprocrea jecorina CBH1 cellulases
Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
59 BASIC BIOLOGICAL SCIENCES↗
Engineering topics
Goedegebuur, Frits
Publications and source records attributed to Goedegebuur, Frits.
Improving the thermal stability of cellobiohydrolase Cel7A from Hypocrea jecorina by directed evolution
Not Available
Goedegebuur, Frits↗
Variant Humicola grisea CBH1.1
Disclosed are variants of Humicola grisea CeI7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Cellulase variants with improved expression, activity and stability, and use thereof
The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.
59 BASIC BIOLOGICAL SCIENCES↗
Beta-glucosidase I variants with improved properties
The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
EGVII endoglucanase and nucleic acids encoding the same
The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.
59 BASIC BIOLOGICAL SCIENCES↗
Variant humicola grisea CBH1.1
Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
59 BASIC BIOLOGICAL SCIENCES↗
CBH1 homologs and varian CBH1 cellulase
Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
59 BASIC BIOLOGICAL SCIENCES↗
Variant Hypocrea jecorina CBH2 cellulases
Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
59 BASIC BIOLOGICAL SCIENCES↗
Variant Hypocrea jecorina CBH1 cellulase
Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
60 APPLIED LIFE SCIENCES↗
Cellulase variants with improved expression, activity and stability, and use thereof
The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.
59 BASIC BIOLOGICAL SCIENCES↗
Cell culture compositions
The present invention provides a novel endoglucanase nucleic acid sequence, designated egl6 (SEQ ID NO:1 encodes the full length endoglucanase; SEQ ID NO:4 encodes the mature form), and the corresponding endoglucanase VI amino acid sequence ("EGVI"; SEQ ID NO:3 is the signal sequence; SEQ ID NO:2 is the mature sequence). The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVI, recombinant EGVI proteins and methods for producing the same.
59 BASIC BIOLOGICAL SCIENCES↗
Variant Humicola grisea CBH1.1
Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
60 APPLIED LIFE SCIENCES↗
EGVII endoglucanase and nucleic acids encoding the same
The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.
60 APPLIED LIFE SCIENCES↗
Variant Hypocrea jercorina CBH1 cellulases
Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
59 BASIC BIOLOGICAL SCIENCES↗
EGVII endoglucanase and nucleic acids encoding the same
The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.
59 BASIC BIOLOGICAL SCIENCES↗
BGL3 beta-glucosidase and nucleic acids encoding the same
The present invention provides a novel .beta.-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.
59 BASIC BIOLOGICAL SCIENCES↗
Variant Humicola grisea CBH1.1
Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
59 BASIC BIOLOGICAL SCIENCES↗