Nanoscale dynamics of cellulose digestion by the cellobiohydrolase TrCel7A
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09 BIOMASS FUELS↗
Engineering topics
Hancock, William O. (ORCID:0000000155478755)
Publications and source records attributed to Hancock, William O. (ORCID:0000000155478755).
Integrated multi-wavelength microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes
We describe a multimodal microscope for visualizing processive enzymes moving on immobilized substrates. The instrument combines interference reflection microscopy (IRM) with multi-wavelength total internal reflectance fluorescence microscopy (TIRFM). The microscope can localize quantum dots with a precision of 2.8 nm at 100 frames/s, and was used to image the dynamics of the cellulase, Cel7a interacting with surface-immobilized cellulose. The instrument, which was built with off-the-shelf components and is controlled by custom software, is suitable for tracking other degradative enzymes such as collagenases, as well as motor proteins moving along immobilized tracks.
09 BIOMASS FUELS↗