Engineering topics
Publications and source records attributed to Hittinger, Christopher Todd.
Methods of making synthetic yeast cells by mating together two diploid (or higher ploidy) yeast species or hybrids to generate multi-ploid yeast hybrids are provided herein. The synthetic yeast cells made by this process and kits for performing the process are also provided.
Provided herein are constructs for genome editing or genetic engineering its fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.