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NASA NTRS2022

Microbes in Microgravity: Animated Educational Videos

Despite the fact that space sciences and biology are both subjects known to inspire students in STEM, the field at the intersection of those subjects-- space biology-- is rarely covered in school curricula. Space Biology gains importance by the day, as NASA launches deep-space missions and private companies initiate the launch of their own orbital space stations. For these reasons, we wanted to inspire students to consider studying biology in space by creating short, informational videos to introduce the topic. Our videos, designed for high school and college students, are focused on microbiology and include four distinct topics: the importance of studying microbes in space, the conditions they experience, how microbes experience microgravity, and how these conditions are simulated on Earth. We hope that space microbiologists use these videos to share the basics about their research, and we hope that students become inspired by the ideas presented in them.

microbiology↗

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Microbial Communities in Microgravity: Simulation in Lab and on the Computer

Microorganisms grow differently in spaceflight than they do on Earth. While much remains unexplained about how microgravity affects microbial growth, one dominant hypothesis is that the lack of density-driven convection in the liquid growth environment makes mixing diffusion-limited, and therefore slower. This is supported by evidence that individual microbial strains experience starvation and acid stress in microgravity. However, if it is true, then microgravity would also have measurable effects on microbes in mixed communities, because many interspecies interactions involve the exchange of soluble metabolites through the medium (cross-feeding). Specifically, cooperative cross-feeding communities would grow more slowly in microgravity, and cooperation would be less stable on evolutionary timescales. Here we describe our efforts to test this hypothesis by simulating microgravity in silico and in the lab, using a model system of Eschericia coli and Salmonella enterica that grow only when they can exchange methionine and acetate. We created CAMDLES (CFD-DEM Artificial Microgravity Developments for Living Ecosystem Simulation) as an extension of CFDEM®coupling software, to carry out computational modeling of biological flows, growth, and mass transfer in microgravity and also in laboratory artificial microgravity devices (rotating wall vessels, RWV). Using CAMDLES, we found distinct differences in growth rates between RWV and true microgravity, and we were able to identify several features, such as spatial distribution, biofilm formation, and product yield parameters, that influence the degree to which RWV growth recapitulates microgravity growth. In addition, we report on the development of a laboratory system for monitoring growth rates and species ratios of the community in RWVs, using fluorescent strains. Pairing CAMDLES with the laboratory model system allows us to generate quantitative predictions about the effects of spaceflight on organisms that will be essential to sustaining human space exploration in the long term.

microbiology↗