Complement Proteins Identify Rapidly Progressive Diabetic Kidney Disease
Not provided.
Urology & Nephrology↗
Engineering topics
Kim, Tae
Publications and source records attributed to Kim, Tae.
The smallest functional antibody fragment: Ultralong CDR H3 antibody knob regions potently neutralize SARS-CoV-2
Cows produce antibodies with a disulfide-bonded antigen-binding domain embedded within ultralong heavy chain third complementarity determining regions. This “knob” domain is analogous to natural cysteine-rich peptides such as knottins in that it is small and stable but can accommodate diverse loops and disulfide bonding patterns. We immunized cattle with SARS-CoV-2 spike and found ultralong CDR H3 antibodies that could neutralize several viral variants at picomolar IC 50 potencies in vitro and could protect from disease in vivo. The independent CDR H3 peptide knobs were expressed and maintained the properties of the parent antibodies. The knob interaction with SARS-CoV-2 spike was revealed by electron microscopy, X-ray crystallography, NMR spectroscopy, and mass spectrometry and established ultralong CDR H3-derived knobs as the smallest known recombinant independent antigen-binding fragment. Unlike other vertebrate antibody fragments, these knobs are not reliant on the immunoglobulin domain and have potential as a new class of therapeutics.
60 APPLIED LIFE SCIENCES↗
Sample Return Container Opening Process Concept for Potential Mars Sample Return
Proposed Mars Sample Return (MSR) missions would require a pristine and secure opening method to ensure sample Contamination Control and backward Planetary Protection. In order to reach the samples, a series of five containers would need to be opened inside of a Mars Returned Sample Handling (MRSH) facility. The process would include opening the Containment Assurance Module (CAM) aboard the Earth Entry Vehicle (EEV), the Secondary Containment Vessel (SCV), Primary Containment Vessel (PCV), and the Orbiting Sample (OS) container, and finally removing and opening the Returned Sample Tube Assemblies (RSTA). Some of these containers can be opened by reversing the mechanical assembly process while others would require destructive means. Several containers are still in a conceptual design phase while others are en route to Mars aboard the M2020 rover. In order to inform the design of the containers that are still in the conceptual stages, a study was performed at each level of the containment assembly. The studies were used to determine the least destructive and most robust methods to disassemble and open the containers while minimizing risk to the samples or the personnel. In addition to the envisioned disassembly and breaching method, contingency plans were made for completing the disassembly if damage to the vehicle and containment system occurred.
Liu, Yang↗
Sample Return Container Opening Process Concept for Potential Mars Sample Return
No abstract provided
Liu, Yang↗
The Heliospheric Current Sheet in the Inner Heliosphere Observed by the Parker Solar Probe
The Parker Solar Probe (PSP) completed its first solar encounter in 2018 November, bringing it closer to the Sun than any previous mission. This allowed in situ investigation of the heliospheric current sheet (HCS) inside the orbit of Venus. The Parker observations reveal a well defined magnetic sector structure placing the spacecraft in a negative polarity region for most of the encounter. The observed current sheet crossings are compared to the predictions of both potential field source surface and magnetohydrodynamic models. All the model predictions are in good qualitative agreement with the observed crossings of the HCS. The models also generally agree that the HCS was nearly parallel with the solar equator during the inbound leg of the encounter and more significantly inclined during the outbound portion. The current sheet crossings at PSP are also compared to similar measurements made by the Wind spacecraft near Earth at 1 au. After allowing for orbital geometry and propagation effects, a remarkable agreement has been found between the observations of these two spacecraft underlying the large-scale stability of the HCS. Finally, the detailed magnetic field and plasma structure of each crossing is analyzed. Marked differences were observed between PSP and Wind measurements in the type of structures found near the HCS. This suggests that significant evolution of these small solar wind structures takes place before they reach 1 au.
Szabo, Adam↗