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Kumar, Raj

Publications and source records attributed to Kumar, Raj.

At least 19 records
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Structural basis for antibody recognition of the proximal MUC16 ectodomain

Mucin 16 (MUC16) overexpression is linked with cancer progression, metastasis, and therapy resistance in high grade serous ovarian cancer and other malignancies. The cleavage of MUC16 forms independent bimodular fragments, the shed tandem repeat sequence which circulates as a protein bearing the ovarian cancer biomarker (CA125) and a proximal membrane-bound component which is critical in MUC16 oncogenic behavior. A humanized, high affinity antibody targeting the proximal ectodomain represents a potential therapeutic agent against MUC16 with lower antigenic potential and restricted human tissue expression. Here, we demonstrate the potential therapeutic versatility of the humanized antibody as a monoclonal antibody, antibody drug conjugate, and chimeric antigen receptor. We report the crystal structures of 4H11-scFv, derived from an antibody specifically targeting the MUC16 C-terminal region, alone and in complex with a 26-amino acid MUC16 segment resolved at 2.36 Å and 2.47 Å resolution, respectively. The scFv forms a robust interaction with an epitope consisting of two consecutive β-turns and a β-hairpin stabilized by 2 hydrogen bonds. The V H -V L interface within the 4H11-scFv is stabilized through an intricate network of 11 hydrogen bonds and a cation-π interaction. Together, our studies offer insight into antibody-MUC16 ectodomain interaction and advance our ability to design agents with potentially improved therapeutic properties over anti-CA125 moiety antibodies.

60 APPLIED LIFE SCIENCES↗
NASA NTRS2021

Science Status of the NASA-ISRO SAR Mission

The NASA-ISRO SAR (NISAR) Mission is entering its second phase of integration and test, preparing for a launch in January 2023. The mission will exploit synthetic aperture radar to map Earth’s surface every 12 days, persistently on ascending and descending portions of the orbit, over all land and ice-covered surfaces. The mission’s primary objectives will be to study Earth land and ice deformation, and ecosystems, in areas of common interest to the US and Indian science communities. This single observatory solution with an L-band (24 cm wavelength) and S-band (10 cm wavelength) radar has a swath of over 240 km at fine resolution, and will operate primarily in a dual-polarimetric mode in an exact repeat orbit. The science teams at NASA and ISRO are working jointly to finalize the joint science plan, calibration and validation plan, and science products, and operational procedures.

Kumar, Raj↗
NASA NTRS2021

NASA-ISRO SAR (NISAR) Mission Status

The National Aeronautics and Space Administration (NASA) in the United States and the Indian Space Research Organisation (ISRO) are developing the NASA-ISRO Synthetic Aperture Radar (NISAR) mission, now planned for launch toward the end of 2022. The mission will exploit synthetic aperture radar to map Earth’s surface every 12 days, persistently on ascending and descending portions of the orbit, over all land and ice-covered surfaces. The mission’s primary objectives will be to study Earth land and ice deformation, and ecosystems, in areas of common interest to the US and Indian science communities. This single observatory solution with an L-band (24 cm wavelength) and S-band (10 cm wavelength) radar has a swath of over 240 km at fine resolution, using full polarimetry where needed. To achieve these unprecedented capabilities, both radars use a reflector-feed system, whereby the feed aperture elements are individually sampled to allow a scan-on-receive (“SweepSAR”) capability at both L-band and S-band. The L-band and S-band electronics and feed apertures, provided by NASA and ISRO respectively, share a common 12-m diameter deployable reflector/boom system, provided by NASA. These two radars, which can operate simultaneously, even with FPGA-based on-board digital beamforming and filtering to reduce data rates, produce prodigious amounts of data. Given the high data rates and ambitious coverage requirements, new technologies for high-rate Ka-band downlink complement these first-of-a-kind radar systems.

Kumar, Raj↗
NASA NTRS2020

NASA-ISRO Synthetic Aperture Radar (NISAR) Mission

NISAR is a multi-disciplinary Earth-observing radar mission that makes global measurements of land surface changes that will greatly improve Earth system models. NISAR data will clarify spatially and temporally complex phenomena, including ecosystem disturbances, ice sheet collapse, and natural hazards including earthquakes, tsunamis, volcanoes, and landslides. It provides societally relevant data that will enable better protection of life and property. The mission, a NASA-ISRO partnership, uses two fully polarimetric SARs, one at L-band (L-SAR) and one at S-band (S-SAR), in exact repeating orbits every 12 days that allows interferometric combination of data on repeated passes. NASA provides the L SAR; a shared deployable reflector; an engineering payload that supports mission-specific data handling, navigation and communication functions; science observation planning and L SAR data processing. ISRO provides the S-SAR, spacecraft, launch vehicle, satellite operations, and S-SAR data processing. The mission will be launched from the Satish Dhawan Space Centre, Sriharikota, India. Mission development has addressed many unique challenges and incorporates many “firsts” for a jointly-developed free-flyer radar science mission.

Sarma, C V H S↗
DOE OSTI2020

Sonochemical synthesis of carbon dots, mechanism, effect of parameters, and catalytic, energy, biomedical and tissue engineering applications

Carbon-based nanomaterials are gaining more and more interest because of their wide range of applications. In this study, carbon dots (CDs) have shown exclusive interest due to unique and novel physicochemical, optical, electrical, and biological properties. Since their discovery, CDs became a promising material for wide range of research applications from energy to biomedical and tissue engineering applications. At same time several new methods have been developed for the synthesis of CDs. Compared to many of these methods, the sonochemical preparation is a green method with advantages such as facile, mild experimental conditions, green energy sources, and feasibility to formulate CDs and doped CDs with controlled physicochemical properties and lower toxicity. In the last five years, the sonochemically synthesized CDs were extensively studied in a wide range of applications. In this review, we discussed the sonochemical assisted synthesis of CDs, doped CDs and their nanocomposites. In addition to the synthetic route, we will discuss the effect of various experimental parameters on the physicochemical properties of CDs; and their applications in different research areas such as bioimaging, drug delivery, catalysis, antibacterial, polymerization, neural tissue engineering, dye absorption, ointments, electronic devices, lithium ion batteries, and supercapacitors. This review concludes with further research directions to be explored for the applications of sonochemical synthesized CDs.

36 MATERIALS SCIENCE↗
NASA NTRS2017

Revision to Global Persistent SAR Sampling with the NASA-ISRO SAR (NISAR) Mission

Abstract—The National Aeronautics and Space Administration (NASA) in the United States and the Indian Space Research Organisation (ISRO) are developing an Earth-orbiting science and applications mission that will exploit synthetic aperture radar to map Earth’s surface every 12 days, persistently on ascending and descending portions of the orbit, over all land and ice-covered surfaces. The mission’s primary objectives will be to study Earth land and ice deformation, and ecosystems, in areas of common interest to the US and Indian science communities. This single spacecraft solution with an L-band (24 cm wavelength) and S-band (10 cm wavelength) radar has a swath of over 240 km at fine resolution, using full polarimetry where needed, uses a reflector-feed system whereby the feed aperture elements are individually sampled to allow a scan-on-receive (“SweepSAR”) capability at both L-band and S-band. This design is in contrast to recent concepts towards large constellations of smaller radar satellites, and is driven by the science requirements for complete coverage over the 12-day repeat cycle, using repeat pass interferometry and polarimetry to measure deformation and surface properties. A single spacecraft with enough aperture, power, duty cycle, and downlink capacity was determined to be a more practical and implementable solution that multiple smaller spacecraft. The use of a single large aperture reflector for both the L- and Sband radars enables both to have comparable performance, leading to overall development and operational efficiencies.

Bhan, Rakesh↗