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Lawrence Koss

Publications and source records attributed to Lawrence Koss.

8 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS2023

CESO 22-4: Parabolic and Suborbital Glovebox in Support of Space Crop Production

Space crop production research approaches and technologies can be validated for microgravity using parabolic or suborbital flight opportunities, and this will save considerable risk, time, and money for implementing new strategies in spaceflight. While the durations of microgravity in these tests are insufficient to grow crops, there are numerous examples of the value of microgravity testing for subsystems such as plant water and nutrient delivery, where multiphase fluid flow can be elucidated in short durations, and horticultural operations, such as harvesting and produce sanitation where containment and contamination can be assessed. Containment in any operational test is essential, as crop operations involve fluids and biological samples, which are potential hazards. This project consists of designing, developing, and constructing a parabolic/suborbital glovebox for experiment containment. The design involves modifications and upgrades to an existing glovebox developed at the University of Louisville. The University of Louisville glovebox was used by KSC researchers for space crop parabolic flight tests in 2021, and lessons learned from that testing have driven design modifications and improvements in the KSC-generated glovebox. Requirements were identified, parts were ordered, an operational science glovebox was fabricated, and a detailed materials specification list was generated. Analyses that are required for flight, remain to be performed to meet airworthiness requirements, and that work will have to be conducted in the future before use in flight.

Food Production↗
NASA NTRS2022

Microgreens Root Zone/Shoot Zone Partitioned Planting Box

To enable sustainable food production in future human exploration missions, plant growth is being studied by the Space Crop Production Team at KSC. Microgreens are good candidates for food supplements and contain specific nutrients that are lacking in the prepackaged diet, including vitamin C and vitamin K. Because they are densely sown, typical growth methods do not allow the ability to distinguish between the levels of evaporation from the rooting substrate and transpiration from the leaves. With larger plants, the root and shoot zone can be separated to distinguish these fluxes and accurate transpiration measurements of plant canopies are feasible. Furthermore, separation of the root and shoot zone may also be beneficial when harvesting microgreens in microgravity as it may reduce microbial contamination of the edible biomass by the roots, which have high microbial loads. Using a root and shoot separator box when harvesting may help with microbial contamination, but these tiny plants are challenging to handle in microgravity, so harvest management remains an open question. The innovation proposed here is a microgreen root/shoot partitioned planting box, which offers a solution to these challenges with accurate gas exchange measurements and a safe microgreen harvest in low gravity environments. Being able to measure transpiration of a microgreen canopy will be important for modeling plant growth in reduced gravity environments, so the first objective was to develop a planting unit with a seal between the root zone and the shoot zone. With an unsuitable harvesting technique, freshly harvested microgreens may add debris to the cabin, so the second objective was to test different harvesting techniques and management approaches associated with this innovation. These two objectives were pursued in parallel since many goals were the same: develop a planting unit that 1) separates the shoots from the roots, 2) allows acceptable germination rate, and 3) allows for seedlings to emerge and develop. What differed was the need to have a seal, which was only applicable for our gas exchange goal, and the need to have an embedded harvesting mechanism and bagging method which only applied to our harvesting goal. Testing of the various harvesting mechanisms and bagging methods was performed during a series of parabolic flights. All parabolic flight procedures took place inside a rented secondary containment chamber (e.g., glovebox) that was developed by the University of Louisville specifically for experiments involving fluids and other materials that may become airborne during reduced gravity flight. Three different harvesting methods and two different bagging collection methods were tested for microgreens. A third bagging method was initially tested but found to be unsuitable. Human factors were also taken into consideration, to identify which harvest and bagging collection methods would be easiest to use with favorable results in microgravity. Three parabolic flight tests were performed in total, one in November 2021 and two in December 2021.

Gioia Massa↗
NASA NTRS2021

Using Electrostatic Principles to Separate Out Nutrients from ECLSS Wastewater Brines

This project will study the use electrostatics to perform electrostatic separation and recovery of Environmental Control and Life Support Systems (ECLSS) waste brines. Currently we have no way to perform nutrient recovery on ECLSS waste brines. This process can give us a competing way to separate sodium from potassium to allow tandem production of acids, bases, and plant fertilizers to run upstream operations and hydroponic systems respectively (when combined with electrodialysis). If we can recover valuable chemicals from brine, then this will decrease resupply from earth, which will allow tremendous (multiple kilograms a day up-mass reduction) cost savings. In addition, using electrostatics for regolith enrichment allow much reduced mining costs for metal production on celestial bodies and allow a smaller mining footprint.

Michael D. Hogue↗
NASA NTRSDate not supplied

Evaluating Microgreens Crop Readiness for Space Production.

Microgreens are small-size, nutrient-rich, and fast-grown crops, which are considered as candidates for future space exploration missions. In particular, the ISS, the Lunar Gateway, and Mars and Lunar missions could benefit from growing microgreens to supplement astronaut diets in the near future. Research at NASA’s Kennedy Space Center has focused on (1) the selection of microgreens compatible species, (2) the evaluation of microgreens food safety, (3) the use of passive wicking, on-demand watering, and hydroponics cultivation, (4) simulated microgravity growth, (5) microgreen canopy gas exchange, and (6) harvesting techniques in microgravity. This presentation summarizes this research. Microgreen species will be evaluated for their yield in relationship to the quantity of inputs – water, seeds, substrate, light intensity, photoperiod, crew time – required for their growth; for their organoleptic and sensory factors in order to down select species that are highly acceptable for humans; and for their microbial loads as detected in their growth environment and the food safety metrics of their edible tissue. Passive wicking, on-demand watering, and hydroponic systems are being studied as an efficient way to deliver essential nutrients and water to microgreens, included in a microgravity environment. Growth studies in simulated microgravity (using 3-dimensional clinostats) will assess microgreens growth relative to that in 1g. Gas exchange studies on microgreens canopies in various airflows will assess their photosynthesis and transpiration. Finally, a series of parabolic flights has enabled the evaluation of different harvesting and bagging techniques in microgravity. Indeed, traditional plant harvesting methods (scissors) in microgravity could generate significant microgreen debris in the space station cabin. Two innovative techniques, coupled to a dedicated bagging method, were designed and evaluated against the control, traditional, harvesting technique. This research was supported by grants from NASA KSC’s Independent Research and Technology Development Program, NASA’s Flight Opportunity Program, NASA Postdoctoral Program Fellowships (L.P. & C.J.) supported by NASA’s Space Biology program, and support from NASA’s Human Research Program.

crop↗