Engineering topics

Lebeau, Juliana

Publications and source records attributed to Lebeau, Juliana.

3 records

DOE OSTI2021

A critical review on the progress and challenges to a more sustainable, cost competitive synthesis of adipic acid

Adipic acid is a key organic diacid intermediate used in nylon manufacturing. It is primarily produced by an industrial process that can form nitrous oxide as a byproduct. Nitrous oxide has a 300-fold higher global warming potential than carbon dioxide, and an estimated 10% of its annual global emissions are a result of adipic acid production. These concerns have led to significant efforts for the development of nitrous oxide mitigation technologies as well as more environmentally friendly routes for adipic acid production. New processes include both advanced chemical and biotechnological routes. Here in this review, we discuss key recent developments in mitigation as well as new technologies. We also provide a critical look at the potential of new technologies to compete with the incumbent process and highlight key remaining technical challenges to the development of greener (environmentally sustainable) and cost competitive (commercially sustainable) processes for adipic acid manufacture.

99 GENERAL AND MISCELLANEOUS↗

DOE OSTI2020

Escherichia coli Cas1/2 Endonuclease Complex Modifies Self-Targeting CRISPR/Cascade Spacers Reducing Silencing Guide Stability

CRISPR-based interference has become common in various applications from genetic circuits to dynamic metabolic control. In E. coli, the native CRISPR Cascade system can be utilized for silencing by deletion of the cas3 nuclease along with expression of guide RNA arrays, where multiple genes can be silenced from a single transcript. We notice the loss of spacer sequences from guide arrays utilized for dynamic silencing. Here, we report that unstable guide arrays are due to expression of the Cas1/2 endonuclease complex. We propose a model wherein basal Cas1/2 endonuclease activity results in the loss of spacers from guide arrays. Subsequently, mutant guide arrays can be amplified through selection. Replacing a constitutive promoter driving Cascade complex expression with a tightly controlled inducible promoter improves guide array stability, while minimizing leaky gene silencing. Additionally, these results demonstrate the potential of Cas1/2 mediated guide deletion as a mechanism to avoid CRISPR based autoimmunity.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2020

A Review of the Biotechnological Production of Methacrylic Acid

Industrial biotechnology can lead to new routes and potentially to more sustainable production of numerous chemicals. We review the potential of biobased routes from sugars to the large volume commodity, methacrylic acid, involving fermentation based bioprocesses. We cover the key progress over the past decade on direct and indirect fermentation based routes to methacrylic acid including both academic as well as patent literature. Finally, we take a critical look at the potential of biobased routes to methacrylic acid in comparison with both incumbent as well as newer greener petrochemical based processes.

59 BASIC BIOLOGICAL SCIENCES↗