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Lee, J. F.

Publications and source records attributed to Lee, J. F..

Fed-batch control based upon the measurement of intracellular NADH

A series of experiments demonstrating that on-line measurements of intracellular NADH by culture fluorescence can be used to monitor and control the fermentation process are described. A distinct advantage of intercellular NADH measurements over other monitoring techniques such as pH and dissolved oxygen is that it directly measures real time events occurring within the cell rather than changes in the environment. When coupled with other measurement parameters, it can provide a finer degree of sophistication in process control.

Armiger, W. B.

Diagnosing a PDS microdensitometer

A number of diagnostic tests are developed for the Photometric Data System PDS 2020G microdensitometer to monitor its performance and to isolate various electromechanical problems. A number of tests which help to diagnose problems with the photometer, positional accuracy and data collection are described. The tests include: (1) scanning a razor blade edge to study the response of the photometer and zero point losses in the coordinate system, (2) scanning a long straight line to evaluate the drunkness of the stage motions, (3) scanning photometric step wedge calibrations to study the response of the photometer, and (4) measurement of a series of high signal to noise plates of the same region of the sky to evaluate the overall performance of the microdensitometer. A variety of electronic tests to isolate electromechanical problems are also performed.

Vanaltena, W.

Improvements to the Photometric response and positional accuracy of the Yale PDS 2020G microdensitometer

The necessity to incorporate several hardware changes to optimize the Yale PDS 2020G microdensitometer for photometric and astrometric research are discussed. The properties of a new high speed photometer and a positional calibration system are described. The new photometer incorporates a high speed logarithmic analog to digital converter with more than 10 times the resolution of the former system and a cycle time of approximately 50 usec. The positional calibration system monitors the drunkenness of the stages with respect to fixed index lines and enables the correction of the +/- 5 micro stage errors to an accuracy of better than 1 micro.

Lee, J. F.