Data for EMSL Project 60517 from February 2023
Explore the source record for details and available documents.
Hu, Dehong↗
Engineering topics
Lei, Chenghong
Publications and source records attributed to Lei, Chenghong.
High Throughput Mapping of Single Molecules’ Redox Potentials on Electrode
By synchronizing electrochemical potential scanning with a single-molecule localization super-resolution fluorescence microscope, kinetic fluorescence changes of hundreds of single molecular redox events were tracked simultaneously with high throughput, and subsequent cross-correlation function analysis mapped single molecules’ redox potentials (times) out on the imaging area from site to site in unprecedented detail by extracting electrochemically induced fluorescence change from apparently random fluorescence on/off blinking. This work paves the way toward mapping redox states at single-molecule levels in high throughput in chemical and biological systems.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Colloidal immobilized protein based on stable colloid of TiO 2 nanoparticles at neutral pH for protein microarray
A stable colloid of titanium dioxide nanoparticles was specially prepared at neutral pH so that horse radish peroxidase as a model protein can interact and be immobilized with colloidal titanium dioxide nanoparticles, while usually titanium dioxide colloid is only stable at acidic condition. Colloidal immobilized peroxidase based on titanium dioxide nanoparticles at neutral pH were highly active and stably dispersible in aqueous solution. The resulting colloidal immobilized peroxidase can be readily printed using a standard microarray printer for facile fabrication of protein microarrays. After printing, the colloidal immobilized protein formed uniform membrane of proteinated nanoparticles with 3-dimensional nanoconfiguration. Finally, the protein microarray sensitivity based on peroxidase-titanium dioxide nanoparticles was nearly 20 times as high as that using conventional technology.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗