A Genetic Study of Nif -Associated Genes in a Hyperthermophilic Methanogen
Methanocaldococcus has been considered a model genus, but genetic tools have not been forthcoming until recently. Here, we develop and illustrate the utility of positive selection with either of two selective agents (simvastatin and neomycin), negative selection, generation of markerless in-frame deletion mutations, and genetic complementation.
59 BASIC BIOLOGICAL SCIENCES↗