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Li, Hong

Publications and source records attributed to Li, Hong.

At least 145 records · Page 8
All sourcesNASA NTRSDOE OSTI
DOE OSTI2016

LncRNA FER1L4 suppresses cancer cell proliferation and cycle by regulating PTEN expression in endometrial carcinoma

Background: Dysregulated long non-coding RNAs (lncRNAs) might exert key roles in pathways associated with endometrial carcinoma (EC) development. This study aims to investigate the new role of lncRNA FER1L4 in EC pathogenesis due to its correlation with phosphatase and tensin homolog (PTEN), one important indicator of EC progression. Methods: Real time PCR was performed to detect the expression of FER1L4 in thirty paired EC samples and two EC cell lines. Plasmid containing FER1L4 was transfected into HEC-50 cells with a relative lower level of FER1L4 expression, followed which PTEN expression and Akt phosphorylation were measured by western blotting. Cell proliferation was analyzed through MTT and colony-formation assays, while cell cycle and apoptosis were determined by flow cytometry. Results: FER1L4 showed significantly downregulation in EC tissues compared to control, which was positively correlated with decreased PTEN expression. Moreover, FER1L4 could promote PTEN expression and inhibit Akt phosphorylation. Additionally, a significant decrease of cell proliferation was observed in FER1L4 overexpressing cells, along with cell cycle arrest at G0/G1 phase and increased proportion of apoptotic cells. Conclusion: FER1L4 not only showed downregulation in EC tissues and cells, but also regulated PTEN expression and Akt signaling, which might contribute to its inhibition on cell proliferation. This study might provide a new potential therapeutic target for EC treatment. - Highlights: •FER1L4 expression was markedly downregulated in EC tissues compared to control. •FER1L4 downregulation was positively correlated with decreased PTEN expression. •FER1L4 could promote PTEN expression and inhibit Akt phosphorylation. • FER1L4 showed significant inhibition on EC cell proliferation. • FER1L4 also induced cell cycle arrest and apoptosis promotion in EC cells.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2016

Battling Btk Mutants With Noncovalent Inhibitors That Overcome Cys481 and Thr474 Mutations

The Bruton’s tyrosine kinase (Btk) inhibitor ibrutinib has shown impressive clinical efficacy in a range of B-cell malignancies. However, acquired resistance has emerged, and second generation therapies are now being sought. Ibrutinib is a covalent, irreversible inhibitor that modifies Cys481 in the ATP binding site of Btk and renders the enzyme inactive, thereby blocking B-cell receptor signal transduction. Not surprisingly, Cys481 is the most commonly mutated Btk residue in cases of acquired resistance to ibrutinib. Mutations at other sites, including Thr474, a gatekeeper residue, have also been detected. Herein, we describe noncovalent Btk inhibitors that differ from covalent inhibitors like ibrutinib in that they do not interact with Cys481, they potently inhibit the ibrutinib-resistant Btk C481S mutant in vitro and in cells, and they are exquisitely selective for Btk. Noncovalent inhibitors such as GNE-431 also show excellent potency against the C481R, T474I, and T474M mutants. X-ray crystallographic analysis of Btk provides insight into the unique mode of binding of these inhibitors that explains their high selectivity for Btk and their retained activity against mutant forms of Btk. This class of noncovalent Btk inhibitors may provide a treatment option to patients, especially those who have acquired resistance to ibrutinib by mutation of Cys481 or Thr474.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2016

Zero-dimensional to three-dimensional nanojoining: current status and potential applications

We report that the continuing miniaturization of microelectronics is pushing advanced manufacturing into nanomanufacturing. Nanojoining is a bottom-up assembly technique that enables functional nanodevice fabrication with dissimilar nanoscopic building blocks and/or molecular components. Various conventional joining techniques have been modified and re-invented for joining nanomaterials. Our review surveys recent progress in nanojoining methods, as compared to conventional joining processes. Examples of nanojoining are given and classified by the dimensionality of the joining materials. At each classification, nanojoining is reviewed and discussed according to materials specialties, low dimensional processing features, energy input mechanisms and potential applications. The preparation of new intermetallic materials by reactive nanoscale multilayer foils based on self-propagating high-temperature synthesis is highlighted. This review will provide insight into nanojoining fundamentals and innovative applications in power electronics packaging, plasmonic devices, nanosoldering for printable electronics, 3D printing and space manufacturing.

36 MATERIALS SCIENCE↗
DOE OSTI2016

Enhancing photovoltaic performance of dye-sensitized solar cells by rare-earth doped oxide of SrAl{sub 2}O{sub 4}:Eu{sup 3+}

Graphical abstract: Current–voltage characteristics of DSSCs based on the photoanodes doping different SrAl{sub 2}O{sub 4}:Eu{sup 3+} and doping 3% SrAl{sub 2}O{sub 4}. - Highlights: • A down-conversion (DC) nanocrystal (SrAl{sub 2}O{sub 4}:Eu{sup 3+}) was synthesized. • The effect of SrAl{sub 2}O{sub 4}:Eu{sup 3+} doped in photoanode in DSSCs was investigated. • SrAl{sub 2}O{sub 4}:Eu{sup 3+} doped in photoanode appeared the better photovoltaic performances. • The dual function of DC and p-type doping effect were explained. - Abstract: SrAl{sub 2}O{sub 4}:Eu{sup 3+} down-conversion (DC) nanocrystals were synthesized by a sol–gel method and then doped in TiO{sub 2} as a photoanode in dye-sensitized solar cells (DSSCs). Differential thermal analysis, fourier transform infrared spectroscopy, X-ray diffraction and Brunauer–Emmet–Teller analysis confirmed the formation of SrAl{sub 2}O{sub 4}:Eu{sup 3+} nanocrystals with diameters of ∼47 nm, pore size of ∼25 nm, sintering temperature of 1300 °C. The photoluminescence and UV–vis absorption spectra of the SrAl{sub 2}O{sub 4}:Eu{sup 3+} revealed a DC from ultraviolet light to visible light which matched the strong absorbing region of the N719 dye. The photoelectric conversion efficiency of the DSSCs with a TiO{sub 2} photoanode doped with 3 wt% SrAl{sub 2}O{sub 4}:Eu{sup 3+} was 20% higher than that with a pure TiO{sub 2} photoanode. This phenomenon could be mainly explained by SrAl{sub 2}O{sub 4}:Eu{sup 3+} nanocrystals’ ability of DC and increased the short-circuit current density. It could be minorly due to the p-type doping effect and slightly improved the open-circuit voltage.

36 MATERIALS SCIENCE↗
DOE OSTI2016

Storage and retrieval of light pulse in coupled quantum wells

In this paper, we propose an effective scheme to create a frequency entangled states based on bound-to-bound inter-subband transitions in an asymmetric three-coupled quantum well structure. A four-subband cascade configuration quantum well structure is illuminated with a pulsed probe field and two continuous wave control laser fields to generate a mixing field. By properly adjusting the frequency detunings and the intensity of coupling fields, the conversion efficiency can reach 100%. A maximum entangled state can be achieved by selecting a proper length of the sample. We also numerically investigate the propagation dynamics of the probe pulse and mixing pulse, the results show that two frequency components are able to exchange energy through a four-wave mixing process. Moreover, by considering special coupling fields, the storage and retrieval of the probe pulse is also numerically simulated.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗
DOE OSTI2016

Strain effects on the work function of an organic semiconductor

Establishing fundamental relationships between strain and work function (WF) in organic semiconductors is important not only for understanding electrical properties of organic thin films, which are subject to both intrinsic and extrinsic strains, but also for developing flexible electronic devices. Here we investigate tensile and compressive strain effects on the WF of rubrene single crystals. Mechanical strain induced by thermal expansion mismatch between the substrate and rubrene is quantified by X-ray diffraction. The corresponding WF change is measured by scanning Kelvin probe microscopy. The WF of rubrene increases (decreases) significantly with in-plane tensile (compressive) strain, which agrees qualitatively with density functional theory calculations. An elastic-to-plastic transition, characterized by a steep rise of the WF, occurs at ~0.05% tensile strain along the rubrene π-stacking direction. Lastly, the results provide the first concrete link between mechanical strain and WF of an organic semiconductor and have important implications for understanding the connection between structural and electronic disorder in soft organic electronic materials.

36 MATERIALS SCIENCE↗
DOE OSTI2016

Cosmic reionization study: principle component analysis after Planck

The study of reionization history plays an important role in understanding the evolution of our universe. It is commonly believed that the intergalactic medium (IGM) in our universe are fully ionized today, however the reionizing process remains to be mysterious. A simple instantaneous reionization process is usually adopted in modern cosmology without direct observational evidence. However, the history of ionization fraction, x{sub e}(z) will influence CMB observables and constraints on optical depth τ. With the mocked future data sets based on featured reionization model, we find the bias on τ introduced by instantaneous model can not be neglected. In this paper, we study the cosmic reionization history in a model independent way, the so called principle component analysis (PCA) method, and reconstruct x{sub e} (z) at different redshift z with the data sets of Planck, WMAP 9 years temperature and polarization power spectra, combining with the baryon acoustic oscillation (BAO) from galaxy survey and type Ia supernovae (SN) Union 2.1 sample respectively. The results show that reconstructed x{sub e}(z) is consistent with instantaneous behavior, however, there exists slight deviation from this behavior at some epoch. With PCA method, after abandoning the noisy modes, we get stronger constraints, and the hints for featured x{sub e}(z) evolution could become a little more obvious.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2016

Ghrelin protects against depleted uranium-induced apoptosis of MC3T3-E1 cells through oxidative stress-mediated p38-mitogen-activated protein kinase pathway

Depleted uranium (DU) mainly accumulates in the bone over the long term. Osteoblast cells are responsible for the formation of bone, and they are sensitive to DU damage. However, studies investigating methods of reducing DU damage in osteoblasts are rarely reported. Ghrelin is a stomach hormone that stimulates growth hormones released from the hypothalamic–pituitary axis, and it is believed to play an important physiological role in bone metabolism. This study evaluates the impact of ghrelin on DU-induced apoptosis of the osteoblast MC3T3-E1 and investigates its underlying mechanisms. The results show that ghrelin relieved the intracellular oxidative stress induced by DU, eliminated reactive oxygen species (ROS) and reduced lipid peroxidation by increasing intracellular GSH levels; in addition, ghrelin effectively suppressed apoptosis, enhanced mitochondrial membrane potential, and inhibited cytochrome c release and caspase-3 activation after DU exposure. Moreover, ghrelin significantly reduced the expression of DU-induced phosphorylated p38-mitogen-activated protein kinase (MAPK). A specific inhibitor (SB203580) or specific siRNA of p38-MAPK could significantly suppress DU-induced apoptosis and related signals, whereas ROS production was not affected. In addition, ghrelin receptor inhibition could reduce the anti-apoptosis effect of ghrelin on DU and reverse the effect of ghrelin on intracellular ROS and p38-MAPK after DU exposure. These results suggest that ghrelin can suppress DU-induced apoptosis of MC3T3-E1 cells, reduce DU-induced oxidative stress by interacting with its receptor, and inhibit downstream p38-MAPK activation, thereby suppressing the mitochondrial-dependent apoptosis pathway. - Highlights: • Ghrelin suppressed DU-induced apoptosis of MC3T3-E1 cells. • Ghrelin inhibited DU-induced oxidative stress and further p38-MAPK activation. • Ghrelin further suppressed mitochondrial-dependent apoptosis pathway. • The anti-oxidation effect of ghrelin was regulated through its receptor. • Ghrelin has the potential for use in drug therapies for DU poisoning.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2015

Bounce inflation cosmology with Standard Model Higgs boson

It is of great interest to connect cosmology in the early universe to the Standard Model of particle physics. In this paper, we try to construct a bounce inflation model with the standard model Higgs boson, where the one loop correction is taken into account in the effective potential of Higgs field. In this model, a Galileon term has been introduced to eliminate the ghost mode when bounce happens. Moreover, due to the fact that the Fermion loop correction can make part of the Higgs potential negative, one naturally obtains a large equation of state(EoS) parameter in the contracting phase, which can eliminate the anisotropy problem. After the bounce, the model can drive the universe into the standard higgs inflation phase, which can generate nearly scale-invariant power spectrum.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2015

Metallothionein deficiency aggravates depleted uranium-induced nephrotoxicity

Depleted uranium (DU) has been widely used in both civilian and military activities, and the kidney is the main target organ of DU during acute high-dose exposures. In this study, the nephrotoxicity caused by DU in metallothionein-1/2-null mice (MT −/−) and corresponding wild-type (MT +/+) mice was investigated to determine any associations with MT. Each MT −/− or MT +/+ mouse was pretreated with a single dose of DU (10 mg/kg, intraperitoneal injection) or an equivalent volume of saline. After 4 days of DU administration, kidney changes were assessed. After DU exposure, serum creatinine and serum urea nitrogen in MT −/− mice significantly increased than in MT +/+ mice, with more severe kidney pathological damage. Moreover, catalase and superoxide dismutase (SOD) decreased, and generation of reactive oxygen species and malondialdehyde increased in MT −/− mice. The apoptosis rate in MT −/− mice significantly increased, with a significant increase in both Bax and caspase 3 and a decrease in Bcl-2. Furthermore, sodium-glucose cotransporter (SGLT) and sodium-phosphate cotransporter (NaPi-II) were significantly reduced after DU exposure, and the change of SGLT was more evident in MT −/− mice. Finally, exogenous MT was used to evaluate the correlation between kidney changes induced by DU and MT doses in MT −/− mice. The results showed that, the pathological damage and cell apoptosis decreased, and SOD and SGLT levels increased with increasing dose of MT. In conclusion, MT deficiency aggravated DU-induced nephrotoxicity, and the molecular mechanisms appeared to be related to the increased oxidative stress and apoptosis, and decreased SGLT expression. - Highlights: • MT −/− and MT +/+ mice were used to evaluate nephrotoxicity of DU. • Renal damage was more evident in the MT −/− mice after exposure to DU. • Exogenous MT also protects against DU-induced nephrotoxicity. • MT deficiency induced more ROS and apoptosis after exposure to DU. • MT deficiency down-regulated SGLT expression after exposure to DU.

60 APPLIED LIFE SCIENCES↗