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Luz M. Calle

Publications and source records attributed to Luz M. Calle.

Silver Foam: A Novel Approach for Long-Term Passive Dosing of Biocide in Spacecraft Potable Water Systems

A spacecraft water disinfection system, suitable for extended length space exploration, should prevent or control the growth of microbes, prevent or limit biofilm formation, and prevent microbiologically influenced corrosion. In addition, the system should have minimal maintenance requirements, be chemically compatible with all materials in contact with the water, be safe for human consumption, and be suitable to be shared across international spacecraft platforms and mission architectures. Silver ions are a proven broad-spectrum potable water biocide under investigation for future exploration missions. The competing technology for dosing silver ions in future water systems is based on actively dosing the ions via electrolytic production. Several challenges with this approach have prompted additional investigations into alternative dosing techniques. Control-release technology is an attractive option for developing a high-reliability passive silver dosing device. This paper describes the development of a nanoparticle/polyurethane (NP/PU) composite foam for the controlled release of silver ions, and is intended to build upon the 2020 International Conference onEnvironmental Systems (ICES) paper number 128. This paper provides the technical background and performance results from the updated silver chloride (AgCl) NP/PU. The ultimate goal of the project is to develop a stable and reliable passive dosing silver ion release device for use in future spacecraft potable water systems.

Tesia D. Irwin↗

Investigation into Space Effects on Biofilm Growth Using Simulated Microgravity

Bacterial growth in liquid media while under microgravity conditions is not well understood. Trends such as a shortened lag phase, longer log phase, slower growth rate, and a higher final population concentration have been noted but the underlying cause remains unclear. Ground-based spaceflight analogs, or simulated microgravity devices, are often employed to achieve different attributes of weightlessness to study effects on bacterial growth. Though these technologies could isolate gravity’s role in various biological processes, they cannot replicate all its effects and underlying mechanisms. Hence, results could be misleading even if they are similar to spaceflight. At the single cell level, it is predicted that bacteria are less gravity-sensitive than larger species. The effects on their immediate environment, including the cell settlement and slower mass transfer of nutrients, might help explain the trends seen in liquid media microgravity studies. Therefore, experimental design factors must be carefully considered when selecting a simulated microgravity device for proper underlying mechanisms and interpretation of results. To verify if the simulated microgravity devices simulate the relevant microgravity conditions for bacterial growth, including the changes in cell settlement and mass transfer of nutrients, a high aspect ratio vessel (HARV) was used with dyes of different density in various simulated microgravity setups. The results will help inform the selection of the proper simulated microgravity device as well as interpretation of subsequent biofilm growth results.

Angie M. Diaz↗

Microgravity Effect on Bacterial Growth: A Literature Review

Gravity is a well-known, but little understood, physical force. It interacts with other physical environmental factors to impact the formation of today’s Earth, and to contribute to biological variations between water and land species. It is also involved with cell differentiation process, and is responsible for setting the boundaries for life and keeping cells small. With their simple structures and small sizes, microbes are expected to be less gravity sensitive than larger species. Nevertheless, various effects of spaceflight on bacterial growth have been reported, but the reports have been sometime inconsistent, and the underlying mechanism remains unclear to many. This paper summarizes the systematic efforts to evaluate the spaceflight effect on microbial growth, highlighting the extracellular mass transfer mechanism. It also provides a critical literature review on the topic to resolve the discrepancy in experimental observations and to further clarify the underlying mechanisms.

Wenyan Li↗