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M W Romeyn

Publications and source records attributed to M W Romeyn.

Legume Organoleptic and Nutritional Analysis

Long-duration missions beyond low Earth orbit will encounter challenges in maintaining adequate nutrition and acceptability in the food system. In situ production of fresh produce can supplement nutrients deficient in the stored diet. Currently there is a limited number of crops that can be reliably grown for space crop production. Recent challenges with Veggie plant growth technical demonstrations (i.e. Tokyo Bekana Chinese cabbage interveinal chlorosis and necrosis when grown under elevated CO2 (~3000ppm) and narrow-band LED lighting) have highlighted the necessity to conduct rigorous ISS-relevant crop screening on the ground. Additionally, crops must be selected to address specific nutritional deficits as identified by HRP, with an emphasis on having a large diversity of crops available to meet nutritional requirements and crew acceptability. A variety of crop types are necessary to address known nutritional deficits in the stored astronaut diet, to include leafy greens (vitamin C, vitamin K, potassium), tomatoes and peppers (vitamin C, potassium, lycopene), and legumes (vitamin B1).Legumes are being evaluated for biological performance and suitability under ISS-like environmental conditions as well as nutritional content and acceptability. Legumes are one of the few pick and eat crop types that is an excellent source of Vitamin B1.In FY21-22 Biological and Physical Sciences (BPS) is funding screening of 32 legume crop cultivars at KSC, 16 of which will be down-selected based on horticultural performance and informal taste testing for further horticultural assessment and analysis. HRP in FY21-22 is funding organoleptic and nutritional analysis of these 16 down-selected legume cultivars to determine the most promising candidates for future space applications. Nutritional analysis will include full elemental, proximate (fat, protein, calories, carbohydrates, ash), and vitamins B1, C, K. This new work is currently postponed due to Covid-19.

L E Spencer

Microgreens Food Safety Evaluation

Microgreens have been recently identified as a new type of pick and eat salad crop that can be utilized in space crop production systems. The majority of traditionally grown leafy green crops can be grown as microgreens, in addition to crops such as legumes (e.g. pea shoots), sunflower, buckwheat, most herbs, and corn, presenting hundreds of microgreen crop options. With a wide variety of flavors, exceptional nutritional density, short growth cycles (7-14 days), and unmatched volume optimization potential, microgreens present as an interesting option for sustainable production of nutritious and flavorful crops in space. The food safety aspects of microgreens have been investigated by USDA in recent years, however, an assessment for crew consumption purposes has not yet been conducted to capture the variety of microgreen cultivar types grown in spaceflight relevant environmental conditions and hardware that could be a part of the astronaut diet. Seed sanitation methods will be developed for each microgreen cultivar to sanitize the seed coat and ensure viable germination and plant growth. Food safety analysis will be performed on 24 different fast-growing cultivars of microgreens by performing aerobic plate counts (APC), fungal counts, and select pathogen screening of edible plant tissue before and after treatment with ProSan sanitizing produce wash. Additional avenues of investigation into microgreens food safety will be undertaken to understand impacts of plant height at time of harvest (able to be manipulated by light spectrum), seed density at time of planting, and role of blue light in food safety metrics of microgreens (n=6-12 microgreen cultivars tested, depending on variable). These findings will be included in the Space Crop Production Hazard Analysis Critical Control Point (HACCP) plan. This new work is currently postponed due to Covid-19.

M E Hummerick

Legume Crop Testing

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LaShelle Spencer