Direct Biomass Conversion through the One-Pot Ionic Liquid Technology.
Abstract not provided.
Achinivu, Ezinne C↗
Engineering topics
Masson, Fabrice
Publications and source records attributed to Masson, Fabrice.
Short-chain ketone production by engineered polyketide synthases in Streptomyces albus
Microbial production of fuels and commodity chemicals has been performed primarily using natural or slightly modified enzymes, which inherently limits the types of molecules that can be produced. Type I modular polyketide synthases (PKSs) are multi-domain enzymes that can produce unique and diverse molecular structures by combining particular types of catalytic domains in a specific order. This catalytic mechanism offers a wealth of engineering opportunities. Here we report engineered microbes that produce various short-chain (C5-C7) ketones using hybrid PKSs. Introduction of the genes into the chromosome of Streptomyces albus enables it to produce >1 g · l -1 of C6 and C7 ethyl ketones and several hundred mg · l -1 of C5 and C6 methyl ketones from plant biomass hydrolysates. Engine tests indicate these short-chain ketones can be added to gasoline as oxygenates to increase the octane of gasoline. Together, it demonstrates the efficient and renewable microbial production of biogasolines by hybrid enzymes.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels
A lignocellulose to bisabolene bioconversion process is demonstrated to be fully compatible with [Ch][Lys].
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
Xylose induces cellulase production in Thermoascus aurantiacus
Not Available
59 BASIC BIOLOGICAL SCIENCES↗
Production of chemical intermediate triacetic acid lactone (TAL) via bacterial fermentation of deconstructed lignin (CRADA Final Report)
The objective of this study was to test the feasibility of using Acinetobacter baylyi as a host strain for production of Triacetic acid lactone (TAL) molecules. A. baylyi was never used as a host strain for the production of an industrial chemical. Moreover, we wanted to test if A. baylyi can consume lignin-based monomers and then convert them to TAL as well. Process optimization in well plate, shake flask and 2L bioreactors along with TAL toxicity studies were planned.
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗