Engineering topics
Masson, P.
Publications and source records attributed to Masson, P..
Circadian oscillations of cytosolic and chloroplastic free calcium in plants
Tobacco and Arabidopsis plants, expressing a transgene for the calcium-sensitive luminescent protein apoaequorin, revealed circadian oscillations in free cytosolic calcium that can be phase-shifted by light-dark signals. When apoaequorin was targeted to the chloroplast, circadian chloroplast calcium rhythms were likewise observed after transfer of the seedlings to constant darkness. Circadian oscillations in free calcium concentrations can be expected to control many calcium-dependent enzymes and processes accounting for circadian outputs. Regulation of calcium flux is therefore fundamental to the organization of circadian systems.
A novel root gravitropism mutant of Arabidopsis thaliana exhibiting altered auxin physiology
A root gravitropism mutant was isolated from the DuPont Arabidopsis thaliana T-DNA insertional mutagenesis collection. This mutant has reduced root gravitropism, hence the name rgr1. Roots of rgr1 are shorter than those of wild-type, and they have reduced lateral root formation. In addition, roots of rgr1 coil clockwise on inclined agar plates, unlike wild-type roots which grow in a wavy pattern. The rgr1 mutant has increased resistance, as measured by root elongation, to exogenously applied auxins (6-fold to indole-3-acetic acid, 3-fold to 2,4-dichlorophenoxyacetic acid, and 2-fold to napthyleneacetic acid). It is also resistant to polar auxin transport inhibitors (2-fold to triiodobenzoic acid and 3- to 5-fold to napthylphthalamic acid). The rgr1 mutant does not appear to be resistant to other plant hormone classes. When grown in the presence of 10(-7) M 2,4-dichlorophenoxyacetic acid, rgr1 roots have fewer root hairs than wild type. All these rgr1 phenotypes are Mendelian recessives. Complementation tests indicate that rgr1 is not allelic to previously characterized agravitropic or auxin-resistant mutants. The rgr1 locus was mapped using visible markers to 1.4 +/- 0.6 map units from the CH1 locus at 1-65.4. The rgr1 mutation and the T-DNA cosegregate, suggesting that rgr1 was caused by insertional gene inactivation.
Global and regional ridge patterns on Mars
A systematic study of Martian wrinkle ridges was performed to synthesize a theory of the planetary interior forces which produced such surface features. The survey was carried out using Mariner-9 and Viking orbiter imagery. Attention was given to the global distributions of ridge types in terms of geologic and tectonic surrounds, and to the orientation of ridges relative to impact basins. High/low relief ridges, ridge wings and rectilinear ridges were examined. Ridge orientation and distribution were found to be controlled by the forces of formation and modification of impact basins. Several other conclusions were reached regarding basin-concentric ridge patterns, regional stress patterns, regions of the most numerous ridges, and the location of a major compressive zone.