Effect of flow on gene regulation in smooth muscle cells and macromolecular transport across endothelial cell monolayers
No abstract available
NASA Discipline Cell Biology↗
Engineering topics
Mcintire, L. V.
Publications and source records attributed to Mcintire, L. V..
Shear stress induced stimulation of mammalian cell metabolism
A flow apparatus was developed for the study of the metabolic response of anchorage dependent cells to a wide range of steady and pulsatile shear stresses under well controlled conditions. Human umbilical vein endothelial cell monolayers were subjected to steady shear stresses of up to 24 dynes/sq cm, and the production of prostacyclin was determined. The onset of flow led to a burst in prostacyclin production which decayed to a long term steady state rate (SSR). The SSR of cells exposed to flow was greater than the basal release level, and increased linearly with increasing shear stress. It is demonstrated that shear stresses in certain ranges may not be detrimental to mammalian cell metabolism. In fact, throughout the range of shear stresses studied, metabolite production is maximized by maximizing shear stress.
Mcintire, L. V.↗
The effect of fluid mechanical stress on cellular arachidonic acid metabolism
The effect of sublytic levels of mechanical perturations of cells on cell metabolism were investigated by analyzing the products of arachidonic acid (used as a marker metabolite) in blood platelets, polymorphonuclear leucocytes, and cultured umbilical-vein endothelial cells after the suspensions of these cells were subjected to a shear stress in a modified viscometer. It is shown that the sublytic levels of mechanical stress stimulated the arachidonic acid metabolism in all these cell types. Possible biological implications of this stress-metabolism coupling are discussed.
Mcintire, L. V.↗
Finite amplitude instability of second-order fluids in plane Poiseuille flow.
The hydrodynamic stability of plane Poiseuille flow of second-order fluids to finite amplitude disturbances is examined using the method of Stuart and Watson as extended by Reynolds and Potter. For slightly non-Newtonian fluids subcritical instabilities are predicted. No supercritical equilibrium states are expected if the entire spectrum of disturbance wavelengths is present. Possible implications with respect to the Toms phenomenon are discussed.
Mcintire, L. V.↗
Hydrodynamic stability of viscoelastic fluids - Importance of fluid model, overstability, and form of disturbance.
A linearized stability analysis has been applied to a fluid flowing in a gravity field between horizontal planes in Couette flow under conditions such that the temperature of the bottom plane exceeds that of the top. It is shown that, under conditions likely to be encountered with polymer solutions, oscillatory instabilities will not be controlling. Criteria are offered for ascertaining when an analysis based upon a second-order fluid model may be expected to yield physically meaningful results. It is also shown that, for the fluid model considered, critical conditions for stability are not changed when disturbances which vary in the flow direction are substituted for those which are a function of the coordinate transverse to the flow.
Mcintire, L. V.↗
On the initiation of melt fracture.
Discussion of a problem encountered in the processing of viscoelastic materials that is caused by the presence of a hydrodynamic instability in the extrusion of polymer melts. The importance of the so-called Weissenberg number in determining the onset of the melt fracture is examined using classical linearized hydrodynamic stability analysis. It is shown that the simple shearing flow of a viscoelastic fluid becomes unstable at a critical value of the Weissenberg number. Implications for the extrusion processing of polymers are reviewed.
Mcintire, L. V.↗
Finite amplitude dynamic motion of viscoelastic materials.
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Yen, H.-C.↗
Finite amplitude dynamic motion of viscoelastic materials.
It is shown that an integral constitutive relation containing a memory function depending on strain tensor invariants can describe the rheological behavior of finite amplitude oscillatory motion of polymer solutions both qualitatively and quantitatively. Values of the material constants are obtained by a numerical technique of simultaneously curve fitting simple shearing viscosity, first normal stress difference, and small amplitude oscillatory motion data.
Yen, H.-C.↗