Glycosyl residue composition of cell wall extracts from tillers of switchgrass WT and PvGAUT4-KD plants
Glycosyl residue composition of cell wall extracts from tillers of switchgrass WT and PvGAUT4-KD plants
bio engineered↗
Engineering topics
Natzke, Jace
Publications and source records attributed to Natzke, Jace.
Glycosyl residue composition and total carbohydrate in cell wall extracts from WT and PvGAUT4-KD lines
Glycosyl residue composition and total carbohydrate in cell wall extracts from WT and PvGAUT4-KD lines
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Glycosyl residue composition of cell wall extracts from stems of P. deltoides WT, vector control and PdGAUT4-KD plants
Glycosyl residue composition of cell wall extracts from stems of P. deltoides WT, vector control and PdGAUT4-KD plants
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Glycosyl residue composition and total carbohydrate of cell wall extracts from WT and PdGAUT4-KD lines
Glycosyl residue composition and total carbohydrate of cell wall extracts from WT and PdGAUT4-KD lines
bio engineered↗
Sugar release Supplmentary Table and notes
Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis
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Life Sciences Reporting summary sugar release
Nature Research reporting summary for manuscript
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Sugar Relese Supplementary Text and Figures
Phylogenetic tree of GAUT Protein Family and gene model, RNAi construct, and relative transcript abundance of GAUT4 in switchgrass, rice and poplar knockdown (KD) lines.
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Glycosyl linkage analysis of fractionated cell walls from switchgrass WT and PvGAUT4-KD lines
Glycosyl linkage analysis of fractionated cell walls from switchgrass WT and PvGAUT4-KD lines
bio engineered↗
Glycosyl linkage analysis of fractionated cell walls from P.deltoides WT and PdGAUT4-KD lines.
Glycosyl linkage analysis of fractionated cell walls from P.deltoides WT and PdGAUT4-KD lines.
bio engineered↗
Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis
Cell walls in crops and trees have been engineered for production of biofuels and commodity chemicals, but engineered varieties often fail multi-year field trials and are not commercialized. In this paper, we engineered reduced expression of a pectin biosynthesis gene (Galacturonosyltransferase 4, GAUT4) in switchgrass and poplar, and find that this improves biomass yields and sugar release from biomass processing. Both traits were maintained in a 3-year field trial of GAUT4-knockdown switchgrass, with up to sevenfold increased saccharification and ethanol production and sixfold increased biomass yield compared with control plants. We show that GAUT4 is an α-1,4-galacturonosyltransferase that synthesizes homogalacturonan (HG). Downregulation of GAUT4 reduces HG and rhamnogalacturonan II (RGII), reduces wall calcium and boron, and increases extractability of cell wall sugars. Finally, decreased recalcitrance in biomass processing and increased growth are likely due to reduced HG and RGII cross-linking in the cell wall.
09 BIOMASS FUELS↗
Significance of Lignin S/G Ratio in Biomass Recalcitrance of Populus trichocarpa Variants for Bioethanol Production
Lignin S/G ratio has been investigated as an important factor in biomass recalcitrance to bioethanol production. Because of the complexity and variety of biomass, recalcitrance was also reportedly influenced by several other factors, such as total lignin content, degree of cellulose polymerization, etc. In addition, the effect of S/G ratio on biomass conversion is not uniform across plant species. Herein, 11 Populus trichocarpa natural variants grown under the same conditions with similar total lignin content were selected to minimize the effects of other factors. The lignin S/G ratio of the selected P. trichocarpa natural variants showed negative correlations with p-hydroxybenzoate (PB) and ..beta..-5 linkage contents, while it had positive ones with ..beta..-O-4 linkage, lignin molecular weight, and ethanol production. This study showed the importance of lignin S/G ratio as an independent recalcitrance factor that may aid future energy crop engineering and biomass conversion strategies.
09 BIOMASS FUELS↗
Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum
During consolidated bioprocessing (CBP), Clostridium thermocellum hydrolyzes several plant cell wall components. Cellulose hydrolysis, specifically, liberates sugars for fermentation, which generates ethanol, acetate, hydrogen, and other products. While several studies indicate that C. thermocellum hydrolyzes carbohydrates in biomass, the structural changes to lignin during CBP remain unclear. In this paper, the whole plant cell walls of untreated and C. thermocellum-treated Populus trichocarpa were characterized using NMR and FTIR. The results suggest that C. thermocellum reduces the β-O-4 linkage content and increases the lignin S/G ratio. Finally, this investigation indicates that C. thermocellum not only modifies lignin in order to access cellulose but also leaves behind a suitable lignin substrate for value-added applications in the cellulosic ethanol production scheme.
59 BASIC BIOLOGICAL SCIENCES↗