Multiplex characterization of microbial traits using dual barcoded nucleic acid fragment expression library
Disclosed herein are barcoded expression libraries comprising a plurality of expression vectors, wherein each expression vector comprises a nucleic acid fragment flanked by a first barcode and a second barcode. Further disclosed herein are methods of making the barcoded expression libraries and methods of conducting functional analysis using the barcoded expression libraries.
Mutalik, Vivek K.↗