De-risking Pretreatment of Microalgae To Produce Fuels and Chemical Co-products
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09 BIOMASS FUELS↗
Engineering topics
Panczak, Bonnie
Publications and source records attributed to Panczak, Bonnie.
Determination of Total, Organic, and Inorganic Carbon and Total Nitrogen in Biological Cultures and Liquid Fraction Process Samples: Laboratory Analytical Procedure (LAP)
This Laboratory Analytical Procedure (LAP) describes the quantitative determination of total organic carbon, inorganic carbon in whole suspended biological samples and in the cell-free supernatant, by combustion and CO 2 detection with a non-dispersive infrared (NDIR) detector. This allows for distinct reporting of soluble and insoluble organic and inorganic carbon. In addition, this procedure covers the quantitative determination of soluble nitrogen in the cell-free supernatant.
09 BIOMASS FUELS↗
Pretreatment of Algae for the Production of Fuels and Chemical Coproducts
Algal biomass is a promising resource for producing renewable fuels and chemicals, but despite decades of research, algal biorefining remains in a pre-commercial state. Recent economic analyses have indicated that high-value co-products are necessary to offset the cost of fuel production, implying the need for algae fractionation technology. One promising fractionation approach is Combined Algal Processing (CAP), which pretreats and extracts algal biomass to produce a fermentable aqueous hydrolysate, an organic lipid fraction, and a residual solids fraction. The CAP approach has historically employed a dilute acid pretreatment to lyse cells and solubilize algal carbohydrates for fermentation of the hydrolysate phase, but we recently identified alternative pretreatments with the potential to decrease costs and environmental impacts, including different implementations of dilute acid pretreatment, dilute alkali pretreatment, enzymatic pretreatment, and flash hydrolysis. We conducted a screening of six different pretreatment approaches across nine algae strains of varying composition, and measured pretreatment effectiveness as a combination of carbon and nitrogen solubilization, lipid extraction yield, and lipid speciation. In this screening, we found traditional dilute acid pretreatment to provide the most robust pretreatment performance, though other pretreatments were competitive, and some performed better for certain strains of algae. These results highlight the interplay between algae composition and pretreatment effectiveness.
algae↗
Tropical Red Macroalgae Cultivation with a Focus on Compositional Analysis
To create carbon efficient sources of bioenergy feedstocks and feedstuff for aquaculture and terrestrial livestock, it is critical to develop and commercialize the most efficient seaweed cultivation approach with a sustainable nutrient input supply. Here, we present data for a novel, onshore tropical macroalgae cultivation system, based on influent deep seawater as the nutrient and carbon sources. Two red algal species were selected, Agardhiella subulata and Halymenia hawaiiana, as the basis for growth optimization. Highest productivity in small-scale cultivation was demonstrated with A. subulata in the 10% deep seawater (64.7 µg N L−1) treatment, growing at up to 26% specific growth rate day−1 with highest yields observed at 247.5 g m−2 day−1 fresh weight. The highest yields for H. hawaiiana were measured with the addition of 10% deep seawater up to 8.8% specific growth rate day−1 and yields at 63.3 g fresh weight m−2 day−1 equivalent. Biomass should be culled weekly or biweekly to avoid density limitations, which likely contributed to a decrease in SGR over time. With a measured 30–40% carbon content of the ash-free dry weight (20–30% of the dry weight) biomass, this translates to an almost 1:1 CO2 capture to biomass ratio. The compositional fingerprint of the high carbohydrate content of both Agardhiella and Halymenia makes for an attractive feedstock for downstream biorefinery applications. By focusing on scaling and optimizing seaweed farming technologies for large-scale onshore farms, the opportunities for yield potential, adaptability to cultivation conditions, and meeting global sustainability goals through novel, carbon-negative biomass sources such as seaweed can be realized.
09 BIOMASS FUELS↗