Glycosyl residue composition of cell wall extracts from tillers of switchgrass WT and PvGAUT4-KD plants
Glycosyl residue composition of cell wall extracts from tillers of switchgrass WT and PvGAUT4-KD plants
bio engineered↗
Engineering topics
Parrott, Wayne A.
Publications and source records attributed to Parrott, Wayne A..
Glycosyl residue composition and total carbohydrate in cell wall extracts from WT and PvGAUT4-KD lines
Glycosyl residue composition and total carbohydrate in cell wall extracts from WT and PvGAUT4-KD lines
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Glycosyl residue composition of cell wall extracts from stems of P. deltoides WT, vector control and PdGAUT4-KD plants
Glycosyl residue composition of cell wall extracts from stems of P. deltoides WT, vector control and PdGAUT4-KD plants
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Glycosyl residue composition and total carbohydrate of cell wall extracts from WT and PdGAUT4-KD lines
Glycosyl residue composition and total carbohydrate of cell wall extracts from WT and PdGAUT4-KD lines
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Sugar release Supplmentary Table and notes
Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis
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Life Sciences Reporting summary sugar release
Nature Research reporting summary for manuscript
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Sugar Relese Supplementary Text and Figures
Phylogenetic tree of GAUT Protein Family and gene model, RNAi construct, and relative transcript abundance of GAUT4 in switchgrass, rice and poplar knockdown (KD) lines.
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Glycosyl linkage analysis of fractionated cell walls from switchgrass WT and PvGAUT4-KD lines
Glycosyl linkage analysis of fractionated cell walls from switchgrass WT and PvGAUT4-KD lines
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Glycosyl linkage analysis of fractionated cell walls from P.deltoides WT and PdGAUT4-KD lines.
Glycosyl linkage analysis of fractionated cell walls from P.deltoides WT and PdGAUT4-KD lines.
bio engineered↗
Embryogenic cell suspensions for high-capacity genetic transformation and regeneration of switchgrass (Panicum virgatum L.)
Abstract Background Switchgrass ( Panicum virgatum L.), a North American prairie grassland species, is a potential lignocellulosic biofuel feedstock owing to its wide adaptability and biomass production. Production and genetic manipulation of switchgrass should be useful to improve its biomass composition and production for bioenergy applications. The goal of this project was to develop a high-throughput stable switchgrass transformation method using Agrobacterium tumefaciens with subsequent plant regeneration. Results Regenerable embryogenic cell suspension cultures were established from friable type II callus-derived inflorescences using two genotypes selected from the synthetic switchgrass variety ‘Performer’ tissue culture lines 32 and 605. The cell suspension cultures were composed of a heterogeneous fine mixture culture of single cells and aggregates. Agrobacterium tumefaciens strain GV3101 was optimum to transfer into cells the pANIC-10A vector with a hygromycin-selectable marker gene and a pporRFP orange fluorescent protein marker gene at an 85% transformation efficiency. Liquid cultures gave rise to embryogenic callus and then shoots, of which up to 94% formed roots. The resulting transgenic plants were phenotypically indistinguishable from the non-transgenic parent lines. Conclusion The new cell suspension-based protocol enables high-throughput Agrobacterium -mediated transformation and regeneration of switchgrass in which plants are recovered within 6–7 months from culture establishment.
Ondzighi-Assoume, Christine A.↗
Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis
Cell walls in crops and trees have been engineered for production of biofuels and commodity chemicals, but engineered varieties often fail multi-year field trials and are not commercialized. In this paper, we engineered reduced expression of a pectin biosynthesis gene (Galacturonosyltransferase 4, GAUT4) in switchgrass and poplar, and find that this improves biomass yields and sugar release from biomass processing. Both traits were maintained in a 3-year field trial of GAUT4-knockdown switchgrass, with up to sevenfold increased saccharification and ethanol production and sixfold increased biomass yield compared with control plants. We show that GAUT4 is an α-1,4-galacturonosyltransferase that synthesizes homogalacturonan (HG). Downregulation of GAUT4 reduces HG and rhamnogalacturonan II (RGII), reduces wall calcium and boron, and increases extractability of cell wall sugars. Finally, decreased recalcitrance in biomass processing and increased growth are likely due to reduced HG and RGII cross-linking in the cell wall.
09 BIOMASS FUELS↗