Formation, characterization and modeling of emergent synthetic microbial communities
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59 BASIC BIOLOGICAL SCIENCES↗
Engineering topics
Pelletier, Dale A.
Publications and source records attributed to Pelletier, Dale A..
Arabidopsis C‐terminal binding protein ANGUSTIFOLIA modulates transcriptional co‐regulation of MYB46 and WRKY33
Not Available
(hemi)biotrophic and necrotrophic pathogens↗
Isolation, Characterization, and Pathogenicity of Two Pseudomonas syringae Pathovars from Populus trichocarpa Seeds
Pseudomonas syringae is a ubiquitous plant pathogen, infecting both woody and herbaceous plants and resulting in devastating agricultural crop losses. Characterized by a remarkable specificity for plant hosts, P. syringae pathovars utilize a number of virulence factors including the type III secretion system and effector proteins to elicit disease in a particular host species. Here, two Pseudomonas syringae strains were isolated from diseased Populustrichocarpa seeds. The pathovars were capable of inhibiting poplar seed germination and were selective for the Populus genus. Sequencing of the newly described organisms revealed similarity to phylogroup II pathogens and genomic regions associated with woody host-associated plant pathogens, as well as genes for specific virulence factors. The host response to infection, as revealed through metabolomics, is the induction of the stress response through the accumulation of higher-order salicylates. Combined with necrosis on leaf surfaces, the plant appears to quickly respond by isolating infected tissues and mounting an anti-inflammatory defense. This study improves our understanding of the initial host response to epiphytic pathogens in Populus and provides a new model system for studying the effects of a bacterial pathogen on a woody host plant in which both organisms are fully genetically sequenced.
59 BASIC BIOLOGICAL SCIENCES↗
Complete Genome Sequence of Starkeya sp. Strain ORNL1, a Soil Alphaproteobacterium Isolated from the Rhizosphere of Populus deltoides
ABSTRACT Starkeya sp. strain ORNL1 is an alphaproteobacterium isolated from the rhizosphere of an Eastern cottonwood tree. Starkeya spp. are physiologically versatile, using a wide range of nutritional and energetic resources and serving important ecological roles in carbon and sulfur cycling. The 6.3-Mb chromosome of Starkeya sp. strain ORNL1 was completely sequenced and will help in understanding nutrient cycles.
59 BASIC BIOLOGICAL SCIENCES↗
Draft Genome Sequence of Tumebacillus sp. Strain BK434, Isolated from the Roots of Eastern Cottonwood
A Gram-positive bacterium was isolated from the root of an eastern cottonwood tree ( Populus deltoides ) in Georgia and identified as a Tumebacillus species with 99% 16S rRNA nucleotide identity to Tumebacillus avium . The genome is 4.6 Mbp and encodes 4,072 proteins and 251 RNAs.
59 BASIC BIOLOGICAL SCIENCES↗
Complex of mutualistic microbes designed to increase plant productivity
The present disclosure provides agricultural compositions and methods of using these compositions to increase plant growth, pathogen resistance and drought tolerance. The agricultural compositions disclosed herein comprise mixtures of mutualistic beneficial fungi such as Laccaria bicolor and Piriformispora indica, and bacterial strains of Pseudomonas.
59 BASIC BIOLOGICAL SCIENCES↗
Draft Genome Sequence of Larkinella sp. Strain BK230, Isolated from Populus deltoides Roots
Larkinella sp. strain BK230, a heterotrophic bacterium of the phylum Bacteroidetes , was isolated from the roots of a field-grown eastern cottonwood tree ( Populus deltoides ) located in Georgia. The draft 7.27-Mb genome has a G+C content of 53.4% and contains 6,026 coding sequences, including 41 tRNA genes.
59 BASIC BIOLOGICAL SCIENCES↗
Relatively rare root endophytic bacteria drive plant resource allocation patterns and tissue nutrient concentration in unpredictable ways
Plant endophytic bacterial strains can influence plant traits such as leaf area and root length. Yet, the influence of more complex bacterial communities in regulating overall plant phenotype is less explored. Here, in two complementary experiments, we tested whether we can predict plant phenotype response to changes in microbial community composition. In the first study, we inoculated a single genotype of Populus deltoides with individual root endophytic bacteria and measured plant phenotype. Next, data from this single inoculation were used to predict phenotypic traits after mixed three-strain community inoculations, which we tested in the second experiment. By itself, each bacterial endophyte significantly but weakly altered plant phenotype relative to noninoculated plants. In a mixture, bacterial strain Burkholderia BT03, constituted at least 98% of community relative abundance. Yet, plant resource allocation and tissue nutrient concentrations were disproportionately influenced by Pseudomonas sp. GM17, GM30, and GM41. We found a 10% increase in leaf mass fraction and an 11% decrease in root mass fraction when replacing Pseudomonas GM17 with GM41 in communities containing both Pseudomonas GM30 and Burkholderia BT03. Our results indicate that interactions among endophytic bacteria may drive plant phenotype over the contribution of each strain individually. Additionally, we have shown that low-abundance strains contribute to plant phenotype challenging the assumption that the dominant strains will drive plant function.
59 BASIC BIOLOGICAL SCIENCES↗