PLANET: Planetary, Lunar, and Asteroid Natural Environment Testbed at NASA Marshall Space Flight Center
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space environment↗
Engineering topics
Peter Bertone
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Planetary Protection at Marshall Space Flight Center
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Planetary Protection↗
Isolation, Identification, and Characterization of MSFC Cleanroom Microbes
Microorganisms can have significant impacts on the success of NASA’s missions, including the integrity of materials, the reliability of scientific results, and crew health. Robust cleaning and sterilization protocols are currently in place in NASA’s facilities, but microbial contamination is unavoidable and its impact on NASA’s missions and science must be minimized. Stringent cleaning and sterilization protocols are currently utilized by NASA, but many projects and materials have constraints on available sterilization procedures. Therefore, it is of utmost importance to be informed of: 1) what specific microorganisms are present, 2) how they may impact mission objectives, and 3) how to mitigate them.
Planetary Protection↗
Overview of Space Environmental Effects (SEE) Team Electrostatic Discharge (ESD) Testing
Electrostatic discharge (ESD) due to the radiation environment encountered during spaceflight missions is a serious risk that must be mitigated by careful testing and design considerations. The MSFC Space Environmental Effects (SEE) Team has world-class facilities for ESD testing along with decades of experience. The Team has recently conducted successful campaigns for several high-profile projects including Europa Clipper, Exploration Upper Stage, Lunar Gateway, Orion, Docking System, and Human Landing System. This presentation will give a brief overview of ESD causes, hazards, test methods and test deliverables.
Peter Bertone↗
Measurement of Secondary Electron Yield from Dielectric Materials
Secondary Electron Yield (SEY) is a material property that plays a fundamental role in material and spacecraft charging. SEY values for dielectric materials (insulators) are crucial inputs to models used to assess mission risk posed by high differential voltages and electrostatic discharge (ESD) on spacecraft. There are only two (non-NASA) facilities that attempt such measurements. The lack of NASA capability results in high cost and long, unpredictable schedules to obtain measurements. Future Artemis crewed lunar surface missions will involve the first use of many insulating materials for which SEY properties are poorly understood or absent entirely. Unconstrained SEY values in models increase uncertainty in charging/ESD risk assessment. The capability for reliable SEY determination will lead to improved charging and ESD risk assessments. We are developing a new capability, using pulsed ultralow (<femtoampere) incident electron beam intensity to eliminate sample charging, coupled with sensitive non-contact surface potential measurements, to measure secondary electron yield (SEY) from insulating materials. Present methods for SEY measurement on insulators suffer from highly inconsistent results due to sample charging from the incident electron beam during the measurement. In this first phase we are leveraging existing EM41 instrumentation, facilities, and expertise to perform proof-of-concept tests.
secondary electron emission↗
Cleanroom Microbes Survive Drying, Vacuum, and Proton Irradiation
Introduction : The goal of planetary protection at NASA is to mitigate the risk of contaminating sensitive target bodies with biological life. While many cleaning procedures have been put in place to reduce bioburden on spacecraft, microbes are experts at evolving to survive harsh conditions. Specifically, the dry, low-nutrient environment of a cleanroom (commonly used for assembly of spacecraft) can represent an environment where extremophiles can survive. Methods : Scientists at NASA MSFC wished to gather a snapshot of the microbial population within a variety of cleanrooms on site. A study was undertaken to collect air, surface, and floor samples from clean-rooms and isolate unique morphologies. From this study, 95 isolates were collected and saved in a microbial library. About 86% of these were identified at least to a genus level. Following identification, 24 microbes were selected, based on a literature review, as potential extremophiles. These were grown in liquid cultures, diluted to a set optical density, washed with water, and then applied to a sterilized Kapton coupon. Droplets were allowed to dry overnight in a biosafety cabinet. Coupons were then installed in a pelletron and pumped down to high vacuum (~1E-6 Torr). Samples were then subjected 100 keV protons at a fluence of 2x10 15 p+/cm 2 up to 4x10 15 p+/cm 2 . Following exposure, samples were returned to the microbiology lab where they were pro-cessed by submerging in water, vortexing, and then plating either droplets or spread plates. Recovery data collected was qualitative with a ranking or +, minor, or – for growth. Some selected radiotolerant strains were sequenced using the Illumina sequencing platform. The resulting genomes were annotated with the Rapid Annotations using Subsystems Technology (RAST) server and analyzed for conserved and unique stress response relevant genomic signatures to identify clues related to specific tolerances. Results and Discussion : After five rounds of proton radiation, we narrowed our isolates to five, non-spore forming bacteria that demonstrated survival: Arthrobacter koreensis, Paenarthrobacter nitroguajacolicus, Mycetocola manganoxydans , and an Erwinia sp. Furthermore, we exposed these four microbes to 254 nm wavelength light at an intensity of 80 W/m 2 at a distance of ~18 cm for 10 minutes. Only A. koreensis demonstrated survival following UV exposure. Finally, we performed whole genome sequencing on the four strains to look for genetic markers of stress resistance. When we compared the genomes of the four strains, we found that genes coding for GGDEF and EAL domains with PAS/PAC sensors were only found in A. koreensis . These domains, modulated by PAS/PAC sensors, are hypothesized to facilitate survival under drying, desiccation, and proton irradiation. Drying and Desiccation : PAS domains sense hydration changes and modulate GGDEF and EAL domain activity to adjust c-di-GMP levels, enhancing resistance to desiccation. For instance, in Pseudomonas aeruginosa , the PAS domain of RbdA modulates activity under varying hydration conditions, affecting stress responses [1]. Proton Irradiation : Proton irradiation causes oxidative stress, leading to ROS generation. PAS domains detect this stress and modulate GGDEF and EAL domains to manage oxidative stress responses. In Shewanella , EAL domain proteins modulated by PAS sensors help bacteria adapt to extreme conditions [2]. These genes upregulate other stress response genes, protecting membrane function, protein stability, DNA repair, and antioxidant defenses. The modulation of c-di-GMP by PAS domains is crucial for bacterial adaptation to stress conditions, enabling dynamic physio-logical adjustments [3]. Understanding these mechanisms provides insights into bacterial stress responses and strategies for controlling bacterial growth [4]. Conclusions : These findings indicate that clean-rooms harbor extremophile microbes that may be able to survive conditions in deep space. Furthermore, while we identified certain stress-response genes that may be at least partly responsible for the phenotypes observed in this study, there are likely unidentified genes or characteristics about A. koreensis , and other bacteria, that may allow them to survive in harsh environments. Future studies will focus on identifying these unknown genes and characteristics, further elucidating the mechanisms of extremophile survival and potentially informing the development of new biotechnologies for space exploration and other extreme environments.
Chelsi Cassilly↗
Update on Planetary, Lunar, & Asteroid Natural Environment Testbed (PLANET)
At ISMSE15, the Planetary, Lunar, & Asteroid Natural Environment Testbed (PLANET) was introduced as an upcoming high-fidelity, combined-effects planetary surface environment laboratory. This year, we will present an update on the facility status, describing the procurement, installation, commissioning of the chamber in Huntsville, Alabama (USA). With NASA’s push to return to the Moon through the Artemis program, there is a clear need for more high-fidelity test chambers that can replicate multiple aspects of the lunar surface, especially the fine, dusty lunar soil known as regolith. The PLANET chamber is designed to fill this gap, enabling research & development, qualification, and verification testing in a combined lunar surface environment, at an affordable price, for government, commercial/industry, and academic partners. Features include a large regolith simulant bed, low energy electron and ion sources to replicate the solar wind, full-spectrum UV and Solar simulation, and a liquid nitrogen cryogenic shroud, all in a high-vacuum environment (as low as 10^-7 mbar). PLANET’s initial focus will be on the lunar environment, but other surface environments (Martian, asteroid, etc.) are also possible to simulate. Besides the environmental instrumentation, PLANET will be equipped with specialized test systems that the Space Environmental Effects Team has developed over the past two years, including an in-situ tribometer and uniform dust distribution system. The chamber is currently being manufactured, with plans to install in May 2024. This will be followed by outfitting and commissioning. The challenges and accomplishments seen during this process will be detailed, and data from the first tests performed in PLANET will be shared with the community.
environmental testing↗