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Roberts, M. S.

Publications and source records attributed to Roberts, M. S..

Microbial Monitoring Challenges and Needs for Mars Applications

The monitoring of microorganisms will be an important part of a mission to Mars. Microbial monitoring equipment will be needed to look for the presence of microorganisms on the planet, to confirm that planetary protection measures are working, to monitor the health of plants, bioreactors and humans living in a habitat and to monitor the performance of the life support systems that will keep them alive during their stay on Mars. Coordinating the different microbial monitoring needs during the early days of mission planning, can provide NASA with equipment that could meet more than one need while also providing complementary analysis options, which can enhance the research capabilities. The early coordination between the different NASA groups that will need microbial monitoring equipment on the surface of Mars, could also make the mission more affordable, as development of the needed equipment could be potentially cost shared.

Roman, M. C.↗

Microbial astronauts: assembling microbial communities for advanced life support systems

Extension of human habitation into space requires that humans carry with them many of the microorganisms with which they coexist on Earth. The ubiquity of microorganisms in close association with all living things and biogeochemical processes on Earth predicates that they must also play a critical role in maintaining the viability of human life in space. Even though bacterial populations exist as locally adapted ecotypes, the abundance of individuals in microbial species is so large that dispersal is unlikely to be limited by geographical barriers on Earth (i.e., for most environments "everything is everywhere" given enough time). This will not be true for microbial communities in space where local species richness will be relatively low because of sterilization protocols prior to launch and physical barriers between Earth and spacecraft after launch. Although community diversity will be sufficient to sustain ecosystem function at the onset, richness and evenness may decline over time such that biological systems either lose functional potential (e.g., bioreactors may fail to reduce BOD or nitrogen load) or become susceptible to invasion by human-associated microorganisms (pathogens) over time. Research at the John F. Kennedy Space Center has evaluated fundamental properties of microbial diversity and community assembly in prototype bioregenerative systems for NASA Advanced Life Support. Successional trends related to increased niche specialization, including an apparent increase in the proportion of nonculturable types of organisms, have been consistently observed. In addition, the stability of the microbial communities, as defined by their resistance to invasion by human-associated microorganisms, has been correlated to their diversity. Overall, these results reflect the significant challenges ahead for the assembly of stable, functional communities using gnotobiotic approaches, and the need to better define the basic biological principles that define ecosystem processes in the space environment. Copyright 2004 Springer-Verlag.

Review↗

Characterization of microbial and chemical composition of shuttle wet waste with permanent gas and volatile organic compound analyses

Solid-waste treatment in space for Advanced Life Support, ALS, applications requires that the material can be safely processed and stored in a confined environment. Many solid-wastes are not stable because they are wet (40-90% moisture) and contain levels of soluble organic compounds that can contribute to the growth of undesirable microorganisms with concomitant production of noxious odors. In the absence of integrated Advanced Life Support systems on orbit, permanent gas, trace volatile organic and microbiological analyses were performed on crew refuse returned from the volume F "wet" trash of three consecutive Shuttle missions (STS-105, 109, and 110). These analyses were designed to characterize the short-term biological stability of the material and assess potential crew risks resulting from microbial decay processes during storage. Waste samples were collected post-orbiter landing and sorted into packaging material, food waste, toilet waste, and bulk liquid fractions deposited during flight in the volume F container. Aerobic and anaerobic microbial loads were determined in each fraction by cultivation on R2A and by acridine orange direct count (AODC). Dry and ash weights were performed to determine both water and organic content of the materials. Experiments to determine the aerobic and anaerobic biostability of refuse stored for varying periods of time were performed by on-line monitoring of CO2 and laboratory analysis for production of hydrogen sulfide and methane. Volatile organic compounds and permanent gases were analyzed using EPA Method TO15 by USEPA et al. [EPA Method TO15, The Determination of Volatile Organic Compounds (VOCs) in Ambient Air using SUMMA, Passivated Canister Sampling and Gas Chromatographic Analysis,1999] with gas chromatography/mass spectrometry and by gas chromatography with selective detectors. These baseline measures of waste stream content, labile organics, and microbial load in the volume F Shuttle trash provide data for waste subsystem analysis and atmospheric management within the ALS Project. Published by Elsevier Ltd on behalf of COSPAR.

NASA Center KSC↗

Observations of 4U 1700-37 with BATSE

The eclipsing binary X-ray source 4U 1700-37 has been continually monitored by the BATSE experiment on the Compton Gamma Ray Observatory since the spring of 1991. Using source measurements at times of Earth occultation, we observe an average (uneclipsed) flux of 0.23 crab in the 20-120 keV band. The flux is highly variable, with occasional flaring behavior on timescales from hundreds of seconds to several hours and intensities as bright as 1 crab. The uneclipsed spectrum is well represented by an optically thin thermal bremsstrahlung model with a temperature of 25 keV independent of source intensity or orbital phase. An upper limit of 4% on the pulse fraction has been obtained for pulse periods between 2 and 700 s. Average orbital light curves from almost 1000 days of occultation measurements have been constructed. These profiles are used to measure: (1) the eclipse semiangle, Theta(sub E) = 28.6 deg +/- 2.1 deg in the 20-120 keV band, and (2) the decrease in orbital period, P(dot)/P = -(3.3 +/- 0.6) x 10(exp -7) 1/ yr. Estimates of system physical parameters are obtained using Monte Carlo simulations to propagate errors in measured and assumed parameters. For the X-ray source mass we find M(sub x) = 2.6(sub -1.4)(sup +2.3) solar mass, and for the mass and radius of the optical companion, M(sub 0) = 30(sub -7)(sup +11) solar mass and R(sub 0) = 18(sub -2)(sup +2) solar radius.

Rubin, B. C.↗

Ultraviolet and optical observations of OB associations in M31

Images of the central and southern parts of the local group Sb spiral galaxy M31 were obtained by the Ultraviolet Imaging Telescope during the Astro-1 Spacelab mission. Stellar photometry is obtained for 611 stars in 59 van den Bergh associations in the near-UV A1 band (central wavelngth 2490 A), and for 130 of these stars in the far UV B1 band (central wavlength 1520 A). In addition, B-band magnitudes for 456 of the stars in approximately 30 associations, together with U- and R-band photometry for approximately 290 stars in approximately 15 associations are determined from CCD images. B-magnitudes from the catalog of Magnier et al. (1992) are given for 137 stars and R-magnitudes for 233 stars. Stellar positions are obtained from the ground based CCD images and from UIT images. Total fluxes in the UV bands are measured for 63 associations . Finding charts are presented in the NUV and FUV bands, as well as, one optical band. Tables give the images utilized in this investigation, the associations observed, with positions, total UV fluxes, extinctions estimated from color-magnitude diagrams, and stellar positions with photometry in up to five bands. UV color magnitude diagrams are presented with extinctions estimated by comparing the observed association color magnitude diagrams with colors and magnitudes of early-type stars computed from the IUE spectral atlas of Fanelli et al. (1992). Comparison with evolutionary models suggests a maximum initial mass approx. 85 solar mass. Ultraviolet extinctions within most M31 OB associations is found to follow an extinction curve similar to that of Hutchings et al. (1992). The variation in the fraction of near-UV stars also measured in the far-UV among the associations is probably caused by variations in age, extinction, and the degree of foreground contamination.

Hill, J. K.↗

The extent of CO in the early-type galaxy NGC 4472

NGC 4472, and E/SO system, is the earliest type normal galaxy with detected CO emission, and here we present additional radio observations in the lines of CO(1-0) and CO(2-1) to determine the distribution and internal properties of this gas. The original detection is reconfirmed, but observations at five surrounding locations and at two other locations in the galaxy do not show the gas to be extended; the total H2 gas mass is estimated to be 4 x 10(exp 7) solar mass. A high CO(1-0)/CO(2-1) brightness temperature ratio is found (greater than 3), which is indicative of subthermal excitation of the CO(2-1) line that can occur at low gas temperatures and low gas densities. Also, upper limits are given for the CO(2-1) fluxes in four other early-type galaxies.

Hutchtmeier, W. K.↗

The dependence on morphology of the gas content in galactic disks

The classification S0 was introduced by Hubble to serve as a description of galaxies whose morphological characteristics seemed to lie between the disk-dominated spirals and the spheroidal elliptical systems. Since then there has been extensive discussion as to whether this classification sequence is also an evolutionary sequence. Many studies have focussed on a particular feature such as the luminosity profile, the bulge-to-disk ratio, or the nature of the interstellar matter, but the question of the evolution remains contentious. Equally contentious is the question of the classification itself. For systems with well-developed disks there usually is no problem. Many spheroidal systems also are unambiguously classified as ellipticals in most catalogs. However, there are a number of early systems which have been reclassified following review using improved optical material. For example, Eder et al. (AJ, 102, 572, 1991) found that many of the S0 galaxies which are rich in neutral hydrogen have faint spiral features. The confusion about classification propagates into the discussion of the properties of early-type systems. Attempts to put the classification system on a quantitative basis have in general been unsuccessful. Recently Sandage (private communication) has reviewed the classification of early systems and has defined a set of sub-classes for these objects. The S0 galaxies are divided into three groups, depending on the prominence of the disk. There are six subdivisions of Sa galaxies, depending upon the relative prominence of knots and other arm-like characteristics. We have explored the total gas content in these objects to see if there is a dependence on the galaxy morphology, as denoted by these new subclasses.

Hogg, D. E.↗

A survey of the properties of early-type galaxies

A compilation of the properties of elliptical and early disk galaxies was completed. In addition to material from the literature, such as Infrared Astronomy Satellite (IRAS) fluxes, the compilation includes recent measurements of HI and CO, as well as a review of the x ray properties by Forman and Jones. The data are used to evaluate the gas content of early systems and to search for correlations with x ray emission. The interstellar medium in early-type galaxies is generally dominated by hot interstellar gas (T approx. 10 to the 7th power K; c.f. the review by Fabbiano 1989 and references therein). In addition, a significant fraction of these galaxies show infrared emission (Knapp, et al., 1989), optical emission lines, and visible dust. Sensitive studies in HI and CO of a number of these galaxies have been completed recently, resulting in several detections, particularly of the later types. Researchers wish to understand the connection among these different forms of the interstellar medium, and to examine the theoretical picture of the fate of the hot gas. To do so, they compiled observations of several forms of interstellar matter for a well-defined sample of early-type galaxies. Here they present a statistical analysis of this data base and discuss the implications of the results.

Bregman, Joel N.↗