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Sharp, G. W.

Publications and source records attributed to Sharp, G. W..

18 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS1999

Calbindin-D(28k) controls [Ca(2+)](i) and insulin release. Evidence obtained from calbindin-d(28k) knockout mice and beta cell lines

The role of the calcium-binding protein, calbindin-D(28k) in potassium/depolarization-stimulated increases in the cytosolic free Ca(2+) concentration ([Ca(2+)](i)) and insulin release was investigated in pancreatic islets from calbindin-D(28k) nullmutant mice (knockouts; KO) or wild type mice and beta cell lines stably transfected and overexpressing calbindin. Using single islets from KO mice and stimulation with 45 mM KCl, the peak of [Ca(2+)](i) was 3.5-fold greater in islets from KO mice compared with wild type islets (p < 0.01) and [Ca(2+)](i) remained higher during the plateau phase. In addition to the increase in [Ca(2+)](i) in response to KCl there was also a significant increase in insulin release in islets isolated from KO mice. Evidence for modulation by calbindin of [Ca(2+)](i) and insulin release was also noted using beta cell lines. Rat calbindin was stably expressed in betaTC-3 and betaHC-13 cells. In response to depolarizing concentrations of K(+), insulin release was decreased by 45-47% in calbindin expressing betaTC cells and was decreased by 70-80% in calbindin expressing betaHC cells compared with insulin release from vector transfected betaTC or betaHC cells (p < 0.01). In addition, the K(+)-stimulated intracellular calcium peak was markedly inhibited in calbindin expressing betaHC cells compared with vector transfected cells (225 nM versus 1,100 nM, respectively). Buffering of the depolarization-induced rise in [Ca(2+)](i) was also observed in calbindin expressing betaTC cells. In summary, our findings, using both isolated islets from calbindin-D(28k) KO mice and beta cell lines, establish a role for calbindin in the modulation of depolarization-stimulated insulin release and suggest that calbindin can control the rate of insulin release via regulation of [Ca(2+)](i).

Non-NASA Center↗
NASA NTRS1985

Shuttle environment database

The proceedings of a workshop on the development of a data base for the Space Shuttle environment are summarized. The data base is intended for use by experimenters in designing scientific payloads for Shuttle missions. The status of currently available data on the Shuttle environment is reviewed, with emphasis given to ten specific topics which are believed to be of concern to Shuttle experiment designers. These topics include: flight dynamics, vibration and acoustics, orbiter motion effects, EMI, and thermal and humidity effects. Attention is also given to: the Shuttle particulate environment; surface interactions inside and on the exterior of the spacecraft, and the natural Shuttle environment. Recent experimental results concerning Vehicle Glow on the spacecraft surface material are discussed in detail. A schematic diagram describing the functional organization of computer hardware for the Shuttle environment data base is provided.

Lauriente, M.↗
NASA NTRS1977

Spacelab payloads

The various payloads planned for Spacelab are discussed with emphasis on the experimental objectives of the payload, the state of planning or development, and the planned flight schedule. Attention is given to payloads involving life sciences, atmospheric and space physics, infrared astronomy, solar physics, ultraviolet/optical astronomy, and high energy astrophysics.

Sharp, G. W.↗
NASA NTRS1973

Proton scattering in the region near the earth's bow shock.

Lockheed spectrometer data from orbits 1 through 20 of Ogo 5 are used to study proton scattering near the bow shock. Correlative UCLA fluxgate magnetometer data from the satellite are used to complete motion studies of the bow shock when spectrometer data are missing. Thirty-eight analyzable sets of multiple shock crossings show 358 shock encounters ranging from 10 to 22 earth radii and spanning sun-earth-satellite angles from 30 to 90 deg. Gross features of the study show that the location and shape of the bow shock are in good agreement with the hydrodynamic model of the bow shock.

Ossakow, S. L.↗
NASA NTRS1972

Neutral density measurements near 400 kilometers by a microphone density gage on Ogo 6 during July 12-15, 1969.

Analysis of persistent density peaks (maxima) measured near 400 km during daytime (1425 LT) by a microphone density gauge on Ogo 6 during July 12 to 15, 1969. Most of the density peaks occurred between geomagnetic latitudes 50 and 61 N and L values 2.3 and 4.7. The average density change in the peaks was 27%. Most of the peaks appeared in the longitude sector from 150 to 350 E. No density peaks were measured in the 50 to 130 E sector during the 3-1/2 day observation period. A persistent pair of density peaks was present on seven successive orbits during July 13 and July 14 (both geomagnetically disturbed days) near geomagnetic latitudes 53 and 61 N, respectively.

Anderson, A. D.↗
NASA NTRS1972

Eclipse and noneclipse differential photoelectron flux.

Differential photoelectron flux in the energy range of 3 to 50 eV has been measured in the lower ionosphere both during the March 7, 1970, solar eclipse and during a period 24 hours earlier. The two measurements were made with identical retarding potential analyzers carried on Nike-Apache rocket flights to a peak altitude of approximately 180 km. The differential electron flux spectrum within totality on the eclipse flight had the same shape but was a factor of 10 smaller in magnitude than that measured on the control day at altitudes between 120 and 180 km, an expected result for an eclipse function decreasing to 1/10 at totality. The differential flux spectrum measured in full sun has the same general energy dependence as that reported by Doering et al. (1970) but is larger by a factor of 2 to 10, depending on altitude.

Knudsen, W. C.↗
NASA NTRS1972

The plasmapause as measured in positive ions.

Use of extensions of the existing theory of magnetospheric convection to describe the dynamics of the plasmasphere and the variation in the plasmapause location. The equatorial local time plane is divided into three separate and physically distinct regions, the bulge region, the nightside region, and the dayside region, corresponding to local times of 1500 to 2200 hrs, 2200 to 0600 hrs, and 0600 to 1500 hrs, respectively. The characteristics predicted by the model in the bulge region are: presence of the bulge at dusk, 1/R to the 4th power radial dependence of plasma concentration, and large fluctuations in plasma density at the plasmapause and plasma detachment. In the nightside region the predicted characteristics are: rapid response to magnetic activity changes, formative region for plasmasphere ripples, and the formative region for the dayside plasmapause location. The model also predicts the dayside plasmasphere characteristics, such as the slow response to magnetic activity changes, and filling of the plasmasphere from the ionosphere, including the formation of a 'double plasmapause.'

Sharp, G. W.↗
NASA NTRS1971

The dayside of the plasmasphere.

The concentrations of H(+) ions in the dayside region of the plasmasphere, measured from March 1968 through February 1969 by the Lockheed light-ion mass spectrometer aboard the OGO 5 satellite, are presented and analyzed. The position of the plasmapause on the dayside appears to be determined by the level of magnetic activity present during the previous corotation of the dayside sector through the formative nightside region. Observations of the buildup of H(+) density versus local time following magnetic storms indicate that H(+) ions flow from the dayside ionosphere into the plasmasphere and plasma trough. Plasmapause density profiles in the afternoon-dusk sector show the effects of the dayside filling from the ionosphere. In addition, several of the dayside profiles display a steep drop in the H(+) density of about a factor of 10 inside the plasmapause position.

Chappell, C. R.↗
NASA NTRS1970

Observations of the plasmapause from OGO 5

Plasmapause observations by ion spectrometer aboard OGO-5 vehicle for early orbits, obtaining O, He and H ion concentration profiles for geomagnetic parameter

Chappell, C. R.↗