RNAseq Analysis of Rodent Spaceflight Experiments Is Confounded by Sample Collection Techniques
Explore the source record for details and available documents.
San-Huei Lai Polo↗
Engineering topics
Sigrid S. Reinsch
Publications and source records attributed to Sigrid S. Reinsch.
GL4U: Using Space Omics Data to Provide Bioinformatics Training for Students and Educators
NASA’s GeneLab project provides researchers open access to space-relevant experiment multi-omics data that can be mined to understand the effects of spaceflight on biological systems. To maximize the number of scientists who understand and utilize GeneLab data and data processing pipelines, GeneLab has created GeneLab for Colleges and Universities (GL4U). GL4U provides space biology-relevant training in bioinformatics to the next generation of scientists through direct and indirect approaches. The GeneLab team plans to host two annual data processing bootcamps, one for college-level students (direct) and one for college educators (indirect – training of trainers), in which participants learn to analyze GeneLab’s space-relevant omics data. The GL4U direct training pilot program was conducted in June 2021. During the pilot, students participated in a week-long bootcamp consisting of space biology-specific lectures and hands-on instruction using Jupyter Notebooks to analyze RNA sequence data. This pilot demonstrated the capacity of GL4U for training young scientists and encouraging data re-use. In June 2022, GL4U partnered with Jet Propulsion Laboratory’s (JPL) Planetary Protection Center of Excellence to conduct the indirect training pilot program by training educators at historically black colleges and universities (HBCUs) and minority serving institutions (MSIs). During the educator pilot, participants received materials, training, and will be provided the necessary compute resources to enable them to run the bootcamp at their home institutions or alternatively to adapt the content to implement within existing courses, thereby extending the reach of this initiative. The GL4U training program provides undergraduate students from underrepresented groups the opportunity to learn about NASA and Space Biology, and to enhance their career prospects by gaining hands-on experience analyzing omics data, a skillset that is highly applicable and marketable in the life sciences. Pre- and post-bootcamp surveys were completed by all participants and show the overwhelming success of the bootcamps.
Amanda M. Saravia-Butler↗
Discovery Through Biospecimen Sharing: The Nasa Biological Institutional Scientific Collection (NBISC)
NASA Ames Research Center has fostered collaborations with other NASA centers, universities, and international space agencies by sharing non-human biospecimens from spaceflight and space-relevant ground experiments since the 1960s. These collaborations have advanced the field of space exploration by helping to maximize the data gained from spaceflight experiments. Non-human tissues archived from experiments funded by NASA’s Space Biology and Human Research Programs (HRP) are available for request through the NASA Life Sciences Portal (NLSP) and distributed by the NASA Biological Institutional Scientific Collection (NBISC). NBISC coordinates closely with the Ames Biospecimen Sharing Program (BSP) which is responsible for sample collection from spaceflight and ground experiments. NBISC currently houses more than 90,000 biospecimens in its facility at NASA Ames. Until recently, NBISC primarily distributed samples from Space Biology funded research. In 2022 NBISC partnered with HRP Space Radiation Element to also archive samples from studies which primarily involve rodents exposed to galactic cosmic radiation simulations at the NASA Space Radiation Laboratory (NSRL) and other analog facilities. In this presentation we will highlight several success stories of analyses carried out using archived NBISC samples received by researchers in recent years and will detail the process for proposing and receiving samples from NBISC. Making available these 90,000+ unique biospecimens to the scientific research community, NBISC not only functions as a resource for storing and distributing non-human biospecimens, but as a warehouse for future discoveries for the benefit of NASA and humankind.
NBISC↗
NASA's Space Health Impacts for the NASA Experience (SHINE) Training Program – Space Radiation Curriculum
In February 2023, the Space Radiation Element of the NASA Human Research Program initiated a virtual, annual space radiation curriculum. The Space Health Impacts for the NASA Experience (SHINE) Space Radiation Didactic Curriculum aims to educate participants not only in the scientific aspects of space radiation but also in the agency’s risk management strategies. SHINE Space Radiation Didactic Curriculum combined weekly seminars by speakers from NASA, other government agencies, academia, and industry, with networking sessions designed to foster collaboration between the competitively selected participants as well as interactions with NASA scientists and HRP funded investigators. The inaugural course ran weekly from February 2023 to August 2023 and was comprised of lectures, less formal coffee hours, and office hours. Each two-hour seminar sessions hosted 1-3 presentations on topics which ranged from the space radiation environment to health effects and countermeasure to granting opportunities. All lectures were recorded and will be published on the THREE (The Health Risks of Extraterrestrial Environments) website for public access (https://three.jsc.nasa.gov/). As a course requirement, participants developed individual or collaborative beam time proposals for real, proposed, or potential experiments at the NASA Space Radiation Laboratory (NSRL) at the Brookhaven National Laboratory. For the 2023 course, 25 participants were selected from a total of 59 applicants. Selected participants were citizens from 8 countries and comprised 5 graduate students, 4 postdocs, 11 scientists and 5 professors/medical doctors. Participants had a wide range of expertise including molecular and cellular biology, microbiology, botany, physiology, engineering, physics, aerospace medicine, planetary science, biostatistics, and modeling. The second annual SHINE training program is scheduled from February to August 2024. In addition, a separate SHINE Space Radiation Practicum session will be held in Fall 2024 at the NSRL. The SHINE Space Radiation Practicum is a unique opportunity that has not been available to the public since the closure of the NASA Space Radiation Summer School in 2017 and will allow a small cohort of participants competitively selected in Fall 2023 to gain hands-on radiation experience.
SHINE↗
The NASA Biological Institutional Scientific Collection (NBISC): Tissue and Microbe Biospecimens to Advance Space Research
The NASA Biological Institutional Scientific Collection (NBISC) is a biorepository of non-human biospecimens from NASA-funded spaceflight investigations and correlative ground studies. The collection has its roots in the 1960s through collaborations by NASA Ames Research Center with other NASA centers, universities, and international space agencies by sharing non-human biospecimens from spaceflight and space-relevant ground experiments. These collaborations have advanced the field of space exploration by helping to maximize the data gained from spaceflight and other NASA-funded experiments. NBISC coordinates closely with the Biospecimen Sharing Program (BSP) which is responsible for organized sample collection from spaceflight and ground rodent experiments. In 2022 NBISC partnered with HRP’s Biospecimen and Tissue Sharing Collection (BTSC) Program to also archive samples from HRP’s Space Radiation Element-funded studies which primarily involve rodents exposed to various radiation protocols at the NASA Space Radiation Laboratory (NSRL) and other analog facilities. To date, more than fifty thousand biospecimens from BTSC have been transferred to NBISC for distribution to the community. In 2023, NBISC embarked on a new Space Biology funded endeavor to create the Space Microbial Culture Collection (SMCC) which will serve as a central repository for microbial isolates associated with space flight, gravitational, and space radiation research. This presentation will highlight several success stories of analyses carried out using archived NBISC samples received by researchers in recent years and will detail the process for proposing and receiving samples from NBISC, BTSC and SMCC. Making available these 150,000+ unique biospecimens to the scientific research community, NBISC not only serves as a repository for storing and distributing non-human biospecimens, but also acts as a resource to enable new discoveries that will benefit NASA and humankind.
NBISC↗