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Smernoff, David T.

Publications and source records attributed to Smernoff, David T..

Method to produce a polysaccharide gel by increasing the pH of the polysaccharide

The present invention provides for a method of producing a polysaccharide gel comprising: (a) culturing or growing a cyanobacterium or a mixture of cyanobacteria species in a medium under conditions such that the cyanobacterium or the mixture of cyanobacteria species produces a polysaccharide or a mixture of polysaccharides, (b) optionally separating the polysaccharide or the mixture of polysaccharides from the medium and/or the cyanobacterium or the mixture of cyanobacteria species, and (c) increasing the pH of the polysaccharide or the mixture of polysaccharides such that the polysaccharide or the mixture of polysaccharides forms a gel.

Mirsiaghi, Mona↗

Controlling Microbial Byproducts using Model-Based Substrate Monitoring and Control Strategies

We have developed a computer-controlled bioreactor system to study various aspects of microbially-mediated nitrogen cycling. The system has been used to investigate methods for controlling microbial denitrification (the dissimilatory reduction of nitrate to N2O and N2) in hydroponic plant growth chambers. Such chambers are key elements of advanced life support systems being designed for use on long duration space missions, but nitrogen use efficiency in them is reduced by denitrification. Control software architecture was designed which permits the heterogeneous control of system hardware using traditional feedback control, and quantitative and qualitative models of various system features. Model-based feed forward control entails prediction of future systems in states and automated regulation of system parameters to achieve desired and avoid undesirable system states. A bacterial growth rate model based on the classic Monod model of saturation kinetics was used to evaluate the response of several individual denitrifying species to varying environmental conditions. The system and models are now being applied to mixed microbial communities harvested from the root zone of a hydroponic growth chamber. The use of a modified Monod organism interaction model was evaluated as a means of achieving more accurate description of the dynamic behavior of the communities. A minimum variance parameter estimation routine was also' used to calibrate the constant parameters in the model by iterative evaluation of substrate (nitrate) uptake and growth kinetics. This representation of processes and interactions aids in the formulation of control laws. The feed forward control strategy being developed will increase system autonomy, reduce crew intervention and limit the accumulation of undesirable waste products (NOx).

Smernoff, David T.↗

Use of Martian resources in a Controlled Ecological Life Support System (CELSS)

Possibile crew life support systems for Mars are reviewed, focusing on ways to use Martian resources as life support materials. A system for bioregenerative life support using photosynthetic organisms, known as the Controlled Ecological Life Support System (CELSS), is examined. The possible use of higher plants or algae to produce oxygen on Mars is investigated. The specific requirements for a CELSS on Mars are considered. The exploitation of water, respiratory gases, and mineral nutrients on Mars is discussed.

Smernoff, David T.↗

Controlled Ecological Life Support System: Regenerative Life Support Systems in Space

A wide range of topics related to the extended support of humans in space are covered. Overviews of research conducted in Japan, Europe, and the U.S. are presented. The methods and technologies required to recycle materials, especially respiratory gases, within a closed system are examined. Also presented are issues related to plant and algal productivity, efficiency, and processing methods. Computer simulation of closed systems, discussions of radiation effects on systems stability, and modeling of a computer bioregenerative system are also covered.

Macelroy, Robert D.↗

Operation of an experimental algal gas exchanger for use in a CELSS

Concepts of a Closed Ecological Life Support System (CELSS) anticipate the use of photosynthetic organisms (higher plants and algae) for air revitalization. The rates of production and uptake of carbon dioxide and oxygen between the crew and the photosynthetic organisms are mismatched. An algal system used for gas exchange only will have the difficulty of an accumulation or depletion of these gases beyond physiologically tolerable limits (in a closed system the mismatch between assimilatory quotient (AQ) and respiratory quotient (RQ) is balanced by the operation of the waste processor). The results are given of a study designed to test the feasibility of using environmental manipulations to maintain physiologically appropriate atmospheres for algae and mice in a gas closed system. Specifically, the atmosphere behavior of this system is considered with algae grown on nitrate or urea and at different light intensities and optical densities. Manipulation of both allow operation of the system in a gas stable manner. Operation of such a system in a CELSS may be useful for reduction of buffer sizes, as a backup system for higher plant air revitalization and to supply extra oxygen to the waste processor or during crew changes.

Smernoff, David T.↗

Controlled Ecological Life Support System. Design, Development, and Use of a Ground-Based Plant Growth Module

Problems of food production by higher plants are addressed. Experimentation requirements and necessary equipment for designing an experimental Controlled Ecological Life Support System (CELSS) Plant Growth Module are defined. A framework is provided for the design of laboratory sized plant growth chambers. The rationale for the development of an informal collaborative effort between investigators from universities and industry and those at Ames is evaluated. Specific research problems appropriate for collaborative efforts are identified.

Macelroy, Robert D.↗

Controlled ecological life support systems: Development of a plant growth module

An effort was made to begin defining the scientific and technical requirements for the design and construction of a ground-based plant growth facility. In particular, science design criteria for the Plant Growth Module (PGM) of the Controlled Ecological Life Support System (CELSS) were determined in the following areas: (1) irradiation parameters and associated equipment affecting plant growth; (2) air flow; (3) planting, culture, and harvest techniques; (4) carbon dioxide; (5) temperature and relative humidity; (6) oxygen; (7) construction materials and access; (8) volatile compounds; (9) bacteria, sterilization, and filtration; (10) nutrient application systems; (11) nutrient monitoring; and (12) nutrient pH and conductivity.

Averner, Mel M.↗

Operation of an experimental algal gas exchanger for use in a CELSS

Concepts of a CELSS anticipate the use of photosynthetic organisms for air revitalization. The rates of production and uptake of carbon dioxide and oxygen between the crew and the photosynthetic organisms are mismatched. An algal system used for gas exchange only will have the difficulty of an accumulation or depletion of these gases beyond physiologically tolerable limits. The results of a study designed to test the feasibility of using environmental manipulations to maintain physiologically appropriate atmospheres for algae (Chlorella pyrenoidosa) and mice (Mus musculus strain DW/J) in a gas-closed system is reported. Specifically, the atmosphere behavior of this system with Chlorella grown on nitrate or urea and at different light intensities and optical densities is considered. Manipulation of both the photosynthetic rate and the assimilatory quotient of the alga has been found to reduce the mismatch of gas requirements and allow operation of the system in a gas-stable manner.

Smernoff, David T.↗

Atmosphere stabilization and element recycle in an experimental mouse-algal system

Life support systems based on bioregeneration rely on the control and manipulation of organisms. Experiments conducted with a gas-closed mouse-algal system designed to investigate principles of photosynthetic gas exchange focus primarily on observing gas exchange phenomena under varying algal environmental conditions and secondarily on studying element cycling through compartments of the experimental system. Inherent instabilities exit between the uptake and release of carbon dioxide CO2 and oxygen O2 by the mouse and algae. Variations in light intensity and cell density alter the photosynthetic rate of the algae and enable maintenance of physiologic concentrations of CO2 and O2. Different nitrogen sources (urea and nitrate) result in different algal assimilatory quotients (AQ). Combinations of photosynthetic rate and AQ ratio manipulations have been examined for their potential in stabilizing atmospheric gas concentrations in the gas-closed algal-mouse system. Elemental mass balances through the experimental systems compartments are being studied with the concurrent development of a mathematical simulation model. Element cycling experiments include quantification of elemental flows through system compartments and wet oxidation of system waste materials for use as an algal nutrient source. Oxidized waste products demonstrate inhibitory properties although dilution has been shown to allow normal growth.

Smernoff, David T.↗