Engineering topics
Snee, Preston T.
Publications and source records attributed to Snee, Preston T..
Time-Resolved 3D Multi-Resolution Microscopy for Real-Time Cellulase Actions in Situ (Final Scientific/Technical Report)
In this Final Report, we are pleased to report that all technical innovation goals as described in the original grant proposal have been achieved. We also provide yet to be published results highlighting the new capabilities enabled by this new technology concept. Also contained in this Final Report is a summary of publications, presentations, software dissemination, as well as Ph.D. dissertations.
Leveraging lifetime information to perform real-time 3D single-particle tracking in noisy environments
A microscopy platform that leverages the arrival time of individual photons to enable 3D single-particle tracking of fast-moving (translational diffusion coefficient of ≃ 3.3 µm 2 /s) particles in high-background environments is reported here. It combines a hardware-based time-gating module, which enables the rate of photon processing to be as high as 100 MHz, with a two-photon excited 3D single-particle tracking confocal microscope to enable high sample penetration depth. Proof-of-principle experiments where single quantum dots are tracked in solutions containing dye-stained cellulose are shown, with tracking performance markedly improved using the hardware-based time-gating module. Furthermore, such a microscope design is anticipated to be of use to a variety of communities who wish to track single-particles in cellular environments, which commonly have high fluorescence and scattering background.