Triblock Elastomeric Vitrimers: Preparation, Morphology, Rheology, and Applications
Not provided.
Polymer Science↗
Engineering topics
Song, Kai
Publications and source records attributed to Song, Kai.
A rapid and sensitive colorimetric assay for the determination of adenosine kinase activity
Highlights: • The absorbance change at 614 nm is positively correlated with the concentration of hydrogen ions. • The spectrophotometry based assay with bromothymol blue as the indicator is feasible for the activity of ADK. • The ADK activity assay is convenient, quick and sensitive, and an excellent alternative for the conventional ADK assays. Adenosine kinase (ADK) plays an important role in the growth and development of organisms. A convenient, quick, reliable, sensitive and low-cost assay for ADK activity is of great significance. Here, we found the reaction system with bromothymol blue as the pH indicator had a maximum absorption peak at 614 nm. The absorbance change in 614 nm was positively correlated with the generated hydrogen ions in the reaction catalyzed by ADK. Then, we demonstrated this assay was feasible for ADK activity. Further, we analyzed the effects of buffer, bromothymol blue concentrations on the sensitivity of the assay, and investigated the sensitivity of ADK contents and adenosine concentration on the assay. Finally, we calculated the K{sub m} and Vmax of ADK from Bombyx mori with this assay. Our results suggested this assay was quick, convenient, reliable, sensitive and economic for the activity of ADK. It is an excellent alternative for the conventional ADK assays.
60 APPLIED LIFE SCIENCES↗
Berkeley Nuclear Data Cloud (BDC) v2.3
The software was developed to serve and curate arbitrarily large datasets comprising data acquired from various mobile platforms. The software is contained in a number of server and client libraries. The former manage the ingestion, indexing, querying, and serving of the data. The latter libraries are distributed for Linux, Mac OSX, and Windows and enable users to interact with data downloaded from the service either in the form of an HDF5 file or streamed in a BSON data chunk. Using the Berkeley Data Cloud, researchers from varying fields can collaborate, compare results and curate both their raw data and the derived products of their analysis.
Cholia, Shreyas↗