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Stephen Lantin

Publications and source records attributed to Stephen Lantin.

8 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS2024

Tracking Community Building in Open Science

Open Science is enabled by a vibrant community of researchers who regularly engage with the data, from its production to its organization, curation, archiving, dissemination, analysis, and publication. This presentation will examine community building in open science. The NASA Open Science Data Repository (OSDR) makes data available to the public following the FAIR (Findability, Accessibility, Interoperability, and Reusability) principles. OSDR takes open science further with the OS Analysis Working Groups (AWGs) that facilitate community development and promotion. The primary activity of each AWG is to establish and validate analytical processes to generate higher-order data from data housed in OSDR. There are a number of these groups on various topics, including the Animal AWG, Plant AWG, Microbial AWG, Multi-Omics AWG, AI/ML AWG, and the Ames Life Sciences Data Archive (ALSDA) AWG. The international volunteers participating in these AWGs come from academia, citizen science initiatives, industry, and government. They include researchers, principal investigators, professors, trained hobbyists, and students from various domains and disciplines. Anyone may request to join the AWGs, and membership requests are vetted monthly by the group organizers before granting admission. Core to membership is demonstrated expertise through records of training, integrity, work in the professed domain(s), and good community standing. Regular virtual meetings are held for each AWG, with a varying cadence depending on the group's needs and goals. AWG communities share their expertise in research including cutting edge tools, software, frameworks, data formats, and libraries accelerating research collectively. This collaborative approach helps community members cross technology gaps and identify emerging challenges. These diverse communities encompass a wide range of individuals hailing from various sectors within the Science Mission Directorate and beyond. They serve as a means to promote and enhance transparency, accessibility, and inclusion. An annual AWG Symposium brings contributors together in person. Participation in AWGs can be synchronous or asynchronous, with some groups performing most of their work in off hours. Participants gain valuable skills and connections that allow them to add value to their communities and new organizations that they join, resulting in an expanded return on investment for the space life science community. Open science is increasingly a federal mandate and initiatives like NASA's Transform to Open Science and instruments like the Decadal Survey of Biological and Physical Sciences in Space demonstrate the need to carefully consider best practices in this domain. Here, we present greater detail about the makeup and participation metrics of the various AWGs affiliated with OSDR and details of successful peer-reviewed publication campaigns.

Christina M Johnson↗
NASA NTRSDate not supplied

3D Printed Materials Characterization for Rapid Prototyping and Plant Growth

Space Biology experimentation but their effects during plant growth and sanitation have not been documented. With further testing we can determine how certain 3D printed materials affect our plants. The way we tested germination included laying two pieces of germination paper down with the printed petri dish insert of the various material and 14 seeds of Outrageous lettuce to fit in the holes of the insert. We kept an even moisture in all parts by wrapping the petri dishes in two layers of parafilm tape. This project tests over 15 different materials to document performance during plant growth scenarios. This research will not only help space biology but also citizens who use these materials to understand the potential of leaching and ill effects. 3D printing is a recent generally accessed technology with new materials coming out every day. It is important to understand how materials could impact the way the plants grow, and the safety of the food grown. As we continue testing these materials, we will also test engineering properties like tension and elasticity. We have a microbiology team working on this project as well to test biofilm formation. From the germination tests we have completed we have seen little impact on germination rate of these seeds. However, some parts absorb moisture. An ideal material is one that doesn’t leach chemicals or microplastics, can withstand high tension and compression, and doesn’t promote microbial growth. This work is funded by NASA. M. Gandhi was funded by MAIANSE.

Mily Gandhi↗
NASA NTRSDate not supplied

Microbial Vessel for Impedance Spectroscopy and Electrochemistry (Mvise): an Extensible, Interoperable Data Acquisition Platform for Liquid Culture Studies in Space Biology Research

The White House Office of Science and Technology Policy (OSTP) has declared 2023 to be the Year of Open Science following an initiative to democratize scientific knowledge. Simultaneously, new sensor technologies have broadened the experimental space available to bioastronautics research. With these open-science goals and technological advances in mind, we have designed and constructed a data acquisition platform for high-precision, real-time monitoring of liquid culture systems. The vessel rig is fitted with six Atlas Scientific probes (micro pH, electrical conductivity, dissolved oxygen, oxidation-reduction potential, liquid temperature, air CO2) and a custom optical density probe similar to the one on BioSentinel’s BioSensor payload. A custom dielectric spectroscopy probe is also planned. The structure of the vessel is resin 3-D printed on a hobbyist-level machine, reducing the production cost and iteration time by over 60% each while increasing extensibility. Data acquisition and storage is controlled with a standalone C state machine-based program running on a Raspberry Pi 3 Model B. When not running headless, an additional program automatically generates and updates plots for live data visualization. Validation of the rig as a data collection system was performed with a yeast liquid culture experiment. While the vessel rig is currently used for standalone experiments, it can also be used as the base perception unit in a self-driving laboratory (SDL). SDLs are high-throughput data collection systems that employ automation and artificial intelligence to conduct and manage routine experiments. Here, we envision an SDL driven by several vessel rigs in which an automated script compares key results, informing the design of future experiments. A vessel rig SDL would streamline many operations, including 1) strain selection for the Lunar Explorer Instrument for space biology Applications (LEIA) investigation and 2) the study of bioregenerative life support systems (BLSS). Ultimately, the datasets that can now be acquired will provide crucial information for accelerating bioastronautics application development in the era of commercial space.

Stephen Lantin↗
NASA NTRSDate not supplied

Sustainable Crop Cultivation in Space Analogs: A BRIDGES Methodology Perspective Through SpaCEA

Sustainable crop cultivation in space holds paramount significance for the support of life in future long-duration missions. This research explores the development and integration of innovative low-cost proof-of-concept (LC-POC) plant growth cabinets tailored for use in space analog missions. By outlining past and current efforts in space farming, this study introduces the SpaCEA Cabinet using BRIDGES framework, establishing a context for reproducible experiments and innovation in plant growth systems. The SpaCEA cabinets can either be delivered in flat packs or assembled on-site, employing cutting-edge methods like 3-D printing and laser cutting. The main objective is to assess how effectively these structures foster crop growth within analog environments while replicating conditions crucial for space exploration. Employing a multi-faceted approach encompassing technical and qualitative dimensions, this project integrates a qualitative investigation where representatives managing analog stations and analog astronauts will partake in interviews and questionnaires to discern specific requirements and challenges within these environments. Insights gained from these engagements will significantly define the final design parameters of updated SpaCEA plant growth cabinets. The practical applicability of these cabinets emphasizes ease of assembly and transportation, addressing the inherent spatial and logistical constraints associated with space missions. Furthermore, the BRIDGES framework ensures the standardization of hardware, software, and data-gathering elements within a unified structure, which utilizes cutting-edge manufacturing technologies for the prototyping and deployment of these cabinets. The anticipated outcomes of this research include the identification of key design considerations and technical specifications for plant growth cabinets tailored to space farming analog systems. This research is poised to contribute valuable knowledge to sustainable space exploration through the development of interoperable plant growth systems for analog environments, advancing research in space crop cultivation which will make up part of a larger bioregenerative life support system.

Advanced Life Support↗
NASA NTRSDate not supplied

Tracking Community Building in Open Science

Open Science is enabled by a vibrant community of researchers who regularly engage with the data, from its production to its organization, curation, archiving, dissemination, analysis, and publication. This presentation will examine community building in open science. The NASA Open Science Data Repository (OSDR) makes data available to the public following the FAIR (Findability, Accessibility, Interoperability, and Reusability) principles. OSDR takes open science further with the OS Analysis Working Groups (AWGs) that facilitate community development and promotion. The primary activity of each AWG is to establish and validate analytical processes to generate higher-order data from data housed in OSDR. There are a number of these groups on various topics, including the Animal AWG, Plant AWG, Microbial AWG, Multi-Omics AWG, AI/ML AWG, and the Ames Life Sciences Data Archive (ALSDA) AWG. The international volunteers participating in these AWGs come from academia, citizen science initiatives, industry, and government. They include researchers, principal investigators, professors, trained hobbyists, and students from various domains and disciplines. Anyone may request to join the AWGs, and membership requests are vetted monthly by the group organizers before granting admission. Core to membership is demonstrated expertise through records of training, integrity, work in the professed domain(s), and good community standing. Regular virtual meetings are held for each AWG, with a varying cadence depending on the group's needs and goals. AWG communities share their expertise in research including cutting edge tools, software, frameworks, data formats, and libraries accelerating research collectively. This collaborative approach helps community members cross technology gaps and identify emerging challenges. These diverse communities encompass a wide range of individuals hailing from various sectors within the Science Mission Directorate and beyond. They serve as a means to promote and enhance transparency, accessibility, and inclusion. An annual AWG Symposium brings contributors together in person. Participation in AWGs can be synchronous or asynchronous, with some groups performing most of their work in off hours. Participants gain valuable skills and connections that allow them to add value to their communities and new organizations that they join, resulting in an expanded return on investment for the space life science community. Open science is increasingly a federal mandate and initiatives like NASA's Transform to Open Science and instruments like the Decadal Survey of Biological and Physical Sciences in Space demonstrate the need to carefully consider best practices in this domain. Here, we present greater detail about the makeup and participation metrics of the various AWGs affiliated with OSDR and details of successful peer-reviewed publication campaigns.

Christina M Johnson↗
NASA NTRSDate not supplied

Sustainable Crop Cultivation in Space Analogs: A BRIDGES Methodology Perspective Through SpaCEA

Sustainable crop cultivation in space holds paramount significance for the support of life in future long-duration missions. This research explores the development and integration of innovative low-cost proof-of-concept (LC-POC) plant growth cabinets tailored for use in space analog missions. By outlining past and current efforts in space farming, this study introduces the SpaCEA Cabinet using BRIDGES framework, establishing a context for reproducible experiments and innovation in plant growth systems. The SpaCEA cabinets can either be delivered in flat packs or assembled on-site, employing cutting-edge methods like 3-D printing and laser cutting. The main objective is to assess how effectively these structures foster crop growth within analog environments while replicating conditions crucial for space exploration. Employing a multi-faceted approach encompassing technical and qualitative dimensions, this project integrates a qualitative investigation where representatives managing analog stations and analog astronauts will partake in interviews and questionnaires to discern specific requirements and challenges within these environments. Insights gained from these engagements will significantly define the final design parameters of updated SpaCEA plant growth cabinets. The practical applicability of these cabinets emphasizes ease of assembly and transportation, addressing the inherent spatial and logistical constraints associated with space missions. Furthermore, the BRIDGES framework ensures the standardization of hardware, software, and data-gathering elements within a unified structure, which utilizes cutting-edge manufacturing technologies for the prototyping and deployment of these cabinets. The anticipated outcomes of this research include the identification of key design considerations and technical specifications for plant growth cabinets tailored to space farming analog systems. This research is poised to contribute valuable knowledge to sustainable space exploration through the development of interoperable plant growth systems for analog environments, advancing research in space crop cultivation which will make up part of a larger bioregenerative life support system.

Advanced Life Support↗
NASA NTRSDate not supplied

Metabolic Vessel for Impedance Spectroscopy and Electrochemistry (MVISE): The Ground Mapping Unit for the Lunar Explorer Instrument for Space Biology Applications (LEIA)​

The BioSensor payload on the upcoming LEIA platform aboard a CLPS lander will carry yeast to the moon to study response to radiation and lunar gravity. The LEIA BioSensor is designed to monitor metabolic activity using absorbance in conjunction with alamarBlue for measuring colorimetric changes as proxy measurement for redox potential. The science data returned from small spacecraft mission modules like LEIA is limited as it relies solely on optical measurements, necessitating a corresponding ground mapping unit that is equipped with multiple electrochemical sensors for accurate mapping of the optical data and operates fully automatically. This technology development work discusses the extensive design and optimization efforts put into the ground mapping unit, MVISE. MVISE is a custom designed, 3D-printed vessel with an agitation system, with six different electrochemical sensor probes, and ports for sample collection and a pressure release valve. The sensors provide real-time data, with dry absorbance measurements aligning with LEIA flight hardware and wet measurements demonstrating invasive sensor design enabling a comparison between the two setups. The 3D printer resin was tested for mechanical robustness, biocompatibility, and resistance to autoclave sterilization. The inner walls were coated with food-grade epoxy, ensuring a smooth finish to prevent microbial lodging and dye staining. The MVISE has successfully passed a week-long leak test and is now undergoing active biology tests. The MVISE prototype will be prepared for radiation tests, with three identical units being tested for varying radiation levels and culture compositions at the NASA Space Radiation Laboratory in November 2024. The integrated sensor approach proposed in this work will enable the accurate mapping of the BioSentinel/LEIA optical flight data to six sensor parameters on the ground unit for better science data return and will enable the first effort to evaluate classical biochemical sensor measurements by comparing and contrasting their responses.

Chinmayee Govinda Raj↗