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Taylor, D. M.

Publications and source records attributed to Taylor, D. M..

At least 19 records
All sourcesNASA NTRSDOE OSTI
NASA NTRS1979

Microbiological evaluation of the mobile biological isolator system

Evaluations on critical components of the mobile biological isolation system were performed. High efficiency particulate air filter efficiency and suit integrity were found to withstand repeated ethylene oxide (ETO) sterilizations. The minimum ETO sterilization time required to inactivate all contaminant organisms was established at four hours. Two days of aerating at 120 F was found to dissipate all harmful ETO residuals from the suit. Donning and doffing procedures were clarified and written specifically for isolation rooms.

Taylor, D. M.↗
NASA NTRS1975

Planetary quarantine: Supporting research and technology

Planetary quarantine strategies for advanced missions are described, along with natural space environment studies and post launch recontamination studies. Spacecraft cleaning and decontamination techniques and assay activities are reviewed. Teflon ribbon experiments and pyrolsis gas-liquid chromatography study are also considered.

Taylor, D. M.↗
NASA NTRS1975

Self sterilization of bodies during outer planet entry

As a body encounters the atmosphere of an outer planet, whether accidentally or by plan, it will be subjected to heat loads which could result in high temperature conditions that render terrestrial organisms on or within the body non-viable. To determine whether an irregularly shaped entering body, consisting of several different materials, would be sterilized during inadvertent entry at high velocity, the thermal response of a typical outer planet spacecraft instrument was studied. The results indicate that the Teflon-insulated cable and electronic circuit boards may not experience sterilizing temperatures during a Jupiter, Saturn, or Titan entry. Another conclusion of the study is that small plastic particles entering Saturn from outer space have wider survival corridors than do those at Jupiter.

Hoffman, A. R.↗
NASA NTRS1975

Consideration of probability of bacterial growth for Jovian planets and their satellites

Environmental parameters affecting growth of bacteria (e.g., moisture, temperature, pH, and chemical composition) were compared with current atmospheric models for Jupiter and Saturn, and with the available physical data for their satellites. Different zones of relative probability of growth were identified for Jupiter and Saturn, with the highest in pressure regions of 1-10 million N/sq m (10 to 100 atmospheres) and 3-30 million N/sq m (30 to 300 atmospheres), respectively. Of the more than two dozen satellites, only the largest (Io, Europa, Ganymede, Callisto, and Titan) were found to be interesting biologically. Titan's atmosphere may produce a substantial greenhouse effect providing increased surface temperatures. Models predicting a dense atmosphere are compatible with microbial growth for a range of pressures at Titan's surface. For Titan's surface the probability of growth would be enhanced if (1) the surface is entirely or partially liquid (water), (2) volcanism (in an ice-water-steam system) is present, or (3) access to internal heat sources is significant.

Taylor, D. M.↗
NASA NTRS1975

A thermal vacuum-UV solar simulator test system for assessing microbiological viability

Microorganisms were exposed to a simulated space environment in order to assess the photobiological effect of broad spectrum, nonionizing solar electromagnetic radiation in terms of viability. A thermal vacuum chamber capable of maintaining a vacuum of 0.000133n/sq m and an ultraviolet rich solar simulator were the main ingredients of the test system. Results to date indicate the system to be capable of providing reliable microbiological data.

Ross, D. S.↗
NASA NTRS1974

Self-sterilization of bodies during outer planet entry

A body encountering the atmosphere of an outer planet is subjected to heat loads which could result in high temperature conditions that render terrestrial organisms on or within the body nonviable. To determine whether an irregularly shaped entering body, consisting of several different materials, would be sterilized during inadvertent entry at high velocity, the thermal response of a typical outer planet spacecraft instrument was studied. The results indicate that the Teflon insulated cable and electronic circuit boards may not experience sterilizing temperatures during a Jupiter, Saturn, or Titan entry. Another conclusion of the study is that small plastic particles entering Saturn from outer space have wider survival corridors than do those at Jupiter.

Hoffman, A. R.↗
NASA NTRS1974

Consideration of probability of bacterial growth for Jovian planets and their satellites

Environmental parameters affecting growth of bacteria are compared with current atmospheric models for Jupiter and Saturn, and with the available physical data for their satellites. Different zones of relative probability of growth are identified for Jupiter and Saturn. Of the more than two dozen satellites, only the largest (Io, Europa, Ganymede, Callisto, and Titan) are found to be interesting biologically. Titan's atmosphere may produce a substantial greenhouse effect providing increased surface temperatures. Models predicting a dense atmosphere are compatible with microbial growth for a range of pressures at Titan's surface. For Titan's surface the probability of growth would be enhanced if: (1) the surface is entirely or partially liquid; (2) volcanism is present; or (3) access to internal heat sources is significant.

Taylor, D. M.↗
NASA NTRS1973

Terrestrial quarantine considerations for unmanned sample return missions

For the purpose of understanding some of the possible implications of a terrestrial quarantine constraint on a mission and for developing a basic approach which can be used to demonstrate compliance beyond that developed for Apollo, a terrestrial quarantine study was performed. It is shown that some of the basic tools developed and used by the planetary quarantine community have applicability to terrestrial quarantine analysis. By using these tools, it is concluded that: (1) the method of biasing the earth aiming point when returning from the planet is necessary but, by itself, may not satisfy terrestrial quarantine constraints; and (2) spacecraft and container design significantly influence contamination transfer.

Hoffman, A. R.↗
NASA NTRS1973

Survival of bacterial isolates exposed to simulated Jovian trapped radiation belt electrons and solar wind protons

With missions to Jupiter, the spacecraft will be exposed for extended durations to solar wind radiation and the Jovian trapped radiation belt. This study is designed to determine the effect of these radiation environments on spacecraft bacterial isolates. The information can be used in the probability of contamination analysis for these missions. A bacterial subpopulation from Mariner Mars 1971 spacecraft (nine spore-forming and three non-spore-forming isolates) plus two comparative organisms, Staphylococcus epidermidis ATCC 17917 and a strain of Bacillus subtilis var. niger, were exposed to 2, 12, and 25 MeV electrons at different doses with simultaneous exposure to a vacuum of 1.3 x 10(-4) N m-2 at 20 and -20 degrees C. The radioresistance of the subpopulation was dependent on the isolate, dose and energy of electrons. Temperature affected the radioresistance of only the spore-forming isolates. Survival data indicated that spores were reduced approximately 1 log/1500 J kg-1 (10 J kg-1=1 krad), while non-spore-forming isolates (micrococci) were reduced 1.5-2 logs/1500 J kg-1 with the exception of an apparent radioresistant isolate whose resistance approached that of the spores. The subpopulation was found to be less resistant to lower energy than to higher energy electrons. The bacterial isolates were exposed to 3 keV protons under the same conditions as the electrons with a total fluence of 1.5 x 10(13) p cm-2 and a dose rate of 8.6 x 10(9) p cm-2 s-1. The results showed that only 20% of S. epidermidis and 45% of B. subtilis populations survived exposure to the 3 keV protons, while the mean survival of the spacecraft subpopulation was 45% with a range from 31.8% (non-spore-former) to 64.8% (non-spore-former). No significant difference existed between spore-forming and non-spore-forming isolates.

Electrons↗
NASA NTRS1972

Survival of bacterial isolates exposed to simulated Jovian trapped radiation belt electrons and solar wind protons

With missions to Jupiter, the spacecraft will be exposed for extended duration to solar wind radiation and the Jovian trapped radiation belt. This study is designed to determine the effect of these radiation environments on spacecraft bacterial isolates. The information can be used in the probability of contamination analysis for these missions. A bacterial subpopulation from Mariner Mars 1971 spacecraft (nine sporeforming and three nonsporeforming isolates) plus two comparative organisms, Staphylococcus epidermidis ATCC 17917 and a strain of Bacillus subtilis var. niger, were exposed to 2-, 12-, and 25-MeV electrons at different doses with simultaneous exposure to a vacuum of 0.0013 N/sqm at 20 and -20 C. The radioresistance of the subpopulation was dependent on the isolate, dose, and energy of electrons. Temperature affected the radioresistance of only the sporeforming isolates. Survival data indicated that spores were reduced approximately 1 log/1500 J/kg, while nonsporeforming isolates (micrococci) were reduced 1.5 to 2 logs/1500 J/kg with the exception of an apparent radioresistant isolate whose resistance approached that of the spores. The subpopulation was found to be less resistant to lower energy than to higher energy electrons.

Taylor, D. M.↗
NASA NTRS1972

Effects of aeolian erosion on microbial release from solids.

This study was initiated to determine the percentage of spores that would be expected to be released from the interior of solid materials by aeolian erosion on a planetary surface. Methyl methacrylate and Eccobond disks were fabricated so that each disk contained approximately 40,000 Bacillus subtilis var. niger spores. The disks were placed in a specially designed sandblasting device and eroded. Exposure periods of 0.5, 2 and 24 hours were investigated using filtered air to accelerate the sand. A series of tests was also conducted for a 0.5 hour period using carbon dioxide. Examination of the erosion products showed that less than 1% of the spores originally contained in the solids was released by aeolian erosion.

Gustan, E. A.↗