Optogenetic Amplification Circuits for Light-Induced Metabolic Control
Not provided.
Biochemistry & Molecular Biology↗
Engineering topics
Toettcher, Jared E.
Publications and source records attributed to Toettcher, Jared E..
Dynamical Modeling of Optogenetic Circuits in Yeast for Metabolic Engineering Applications
Not provided.
Biochemistry & Molecular Biology↗
Design and Characterization of Rapid Optogenetic Circuits for Dynamic Control in Yeast Metabolic Engineering
Not provided.
Biochemistry & Molecular Biology↗
Optogenetic control of protein binding using light-switchable nanobodies
A growing number of optogenetic tools have been developed to reversibly control binding between two engineered protein domains. In contrast, relatively few tools confer lightswitchable binding to a generic target protein of interest. Such a capability would offer substantial advantages, enabling photoswitchable binding to endogenous target proteins in cells or light-based protein purification in vitro. Here, we report the development of optonanobodies (OptoNBs), a versatile class of chimeric photoswitchable proteins whose binding to proteins of interest can be enhanced or inhibited upon blue light illumination. We find that OptoNBs are suitable for a range of applications including reversibly binding to endogenous intracellular targets, modulating signaling pathway activity, and controlling binding to purified protein targets in vitro. This work represents a step towards programmable photoswitchable regulation of a wide variety of target proteins.
59 BASIC BIOLOGICAL SCIENCES↗