Engineering topics
Tran, Duc
Publications and source records attributed to Tran, Duc.
Single-cell quantification of ribosome occupancy in early mouse development
Translation regulation is critical for early mammalian embryonic development. However, previous studies had been restricted to bulk measurements, precluding precise determination of translation regulation including allele-specific analyses. Here, to address this challenge, we developed a novel microfluidic isotachophoresis (ITP) approach, named RIBOsome profiling via ITP (Ribo-ITP), and characterized translation in single oocytes and embryos during early mouse development. We identified differential translation efficiency as a key mechanism regulating genes involved in centrosome organization and N 6 -methyladenosine modification of RNAs. Our high-coverage measurements enabled, to our knowledge, the first analysis of allele-specific ribosome engagement in early development. These led to the discovery of stage-specific differential engagement of zygotic RNAs with ribosomes and reduced translation efficiency of transcripts exhibiting allele-biased expression. By integrating our measurements with proteomics data, we discovered that ribosome occupancy in germinal vesicle-stage oocytes is the predominant determinant of protein abundance in the zygote. The Ribo-ITP approach will enable numerous applications by providing high-coverage and high-resolution ribosome occupancy measurements from ultra-low input samples including single cells.
Mouse genomic associations with in vitro sensitivity to simulated space radiation
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Enhanced Electrolyte Transport and Kinetics Mitigate Graphite Exfoliation and Li Plating in Fast–Charging Li–Ion Batteries
Despite significant progress in energy retention, lithium-ion batteries (LIBs) face untenable reductions in cycle life under extreme fast-charging (XFC) conditions, which primarily originate from a variety of kinetic limitations between the graphite anode and the electrolyte. Through quantitative Li + loss accounting and comprehensive materials analyses, it is directly observed that the operation of LIB pouch cells at 4 C||C/3 (charging||discharging) results in Li plating, disadvantageous solid-electrolyte-interphase formation, and solvent co-intercalation leading to interstitial decomposition within graphite layers. It is found that these failure modes originate from the insufficient properties of conventional electrolytes, where employing a designed ester-based electrolyte improved the capacity retention of these cells from 55.9% to 88.2% after 500 cycles when operated at the aforementioned conditions. These metrics are the result of effective mitigation of the aforementioned failure modes due to superior Li + transport and desolvation characteristics demonstrated through both experimental and computational characterization. Finally, this work reveals the vital nature of electrolyte design to XFC performance.
Current Challenges in Efficient Lithium‐Ion Batteries’ Recycling: A Perspective
Abstract Li‐ion battery (LIB) recycling has become an urgent need with rapid prospering of the electric vehicle (EV) industry, which has caused a shortage of material resources and led to an increasing amount of retired batteries. However, the global LIB recycling effort is hampered by various factors such as insufficient logistics, regulation, and technology readiness. Here, the challenges associated with LIB recycling and their possible solutions are summarized. Different aspects such as recycling/upcycling techniques, worldwide government policies, and the economic and environmental impacts are discussed, along with some practical suggestions to overcome these challenges for a promising circular economy for LIB materials. Some potential strategies are proposed to convert such challenges into opportunities to maintain the global expansion of the EV and other LIB‐dependent industries.
Correction of sideslip-induced static pressure errors in flight-test measurements
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Sideslip-induced static pressure errors in flight-test measurements
During lateral flight-test maneuvers of a V/STOL research aircraft, large errors in static pressure were observed. An investigation of the data showed a strong correlation of the pressure record with variations in sideslip angle. The sensors for both measurements were located on a standard air-data nose boom. An algorithm based on potential flow over a cylinder that was developed to correct the pressure record for sideslip-induced errors is described. In order to properly apply the correction algorithm, it was necessary to estimate and correct the lag error in the pressure system. The method developed for estimating pressure lag is based on the coupling of sideslip activity into the static ports and can be used as a standard flight-test procedure. The estimation procedure is discussed and the corrected static-pressure record for a typical lateral maneuver is presented. It is shown that application of the correction algorithm effectively attenuates sideslip-induced errors.
Sideslip-induced static pressure errors in flight-test measurements
During lateral flight-test maneuvers of a V/STOL research aircraft, large errors in static pressure were observed. An investigation of the data showed a strong correlation of the pressure record with variations in sideslip angle. The sensors for both measurements were located on a standard air-data nose boom. This paper descries an algorithm based on potential flow over a cylinder that was developed to correct the pressure record for sideslip-induced errors. In order to properly apply the correction algorithm, it was necessary to estimate and correct the lag error in the pressure system. The method developed for estimating pressure lag is based on the coupling of sideslip activity into the static ports and can be used as a standard flight-test procedure. The paper discusses the estimation procedure and presents the corrected static-pressure record for a typical lateral maneuver. It is shown that application of the correction algorithm effectifvely attenuates sideslip-induced errors.